Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4E2F

Crystal Structure of E. coli Aspartate Transcarbamoylase K164E/E239K Mutant in an intermediate state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004070	molecular_function	aspartate carbamoyltransferase activity
A	0004088	molecular_function	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
A	0006221	biological_process	pyrimidine nucleotide biosynthetic process
A	0006520	biological_process	amino acid metabolic process
A	0006541	biological_process	glutamine metabolic process
A	0009347	cellular_component	aspartate carbamoyltransferase complex
A	0016597	molecular_function	amino acid binding
A	0016740	molecular_function	transferase activity
A	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
A	0042802	molecular_function	identical protein binding
A	0044205	biological_process	'de novo' UMP biosynthetic process
A	0070207	biological_process	protein homotrimerization
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
B	0006221	biological_process	pyrimidine nucleotide biosynthetic process
B	0008270	molecular_function	zinc ion binding
B	0009347	cellular_component	aspartate carbamoyltransferase complex
B	0046872	molecular_function	metal ion binding
C	0004070	molecular_function	aspartate carbamoyltransferase activity
C	0004088	molecular_function	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
C	0006221	biological_process	pyrimidine nucleotide biosynthetic process
C	0006520	biological_process	amino acid metabolic process
C	0006541	biological_process	glutamine metabolic process
C	0009347	cellular_component	aspartate carbamoyltransferase complex
C	0016597	molecular_function	amino acid binding
C	0016740	molecular_function	transferase activity
C	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
C	0042802	molecular_function	identical protein binding
C	0044205	biological_process	'de novo' UMP biosynthetic process
C	0070207	biological_process	protein homotrimerization
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
D	0006221	biological_process	pyrimidine nucleotide biosynthetic process
D	0008270	molecular_function	zinc ion binding
D	0009347	cellular_component	aspartate carbamoyltransferase complex
D	0046872	molecular_function	metal ion binding
E	0004070	molecular_function	aspartate carbamoyltransferase activity
E	0004088	molecular_function	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
E	0006221	biological_process	pyrimidine nucleotide biosynthetic process
E	0006520	biological_process	amino acid metabolic process
E	0006541	biological_process	glutamine metabolic process
E	0009347	cellular_component	aspartate carbamoyltransferase complex
E	0016597	molecular_function	amino acid binding
E	0016740	molecular_function	transferase activity
E	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
E	0042802	molecular_function	identical protein binding
E	0044205	biological_process	'de novo' UMP biosynthetic process
E	0070207	biological_process	protein homotrimerization
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
F	0006221	biological_process	pyrimidine nucleotide biosynthetic process
F	0008270	molecular_function	zinc ion binding
F	0009347	cellular_component	aspartate carbamoyltransferase complex
F	0046872	molecular_function	metal ion binding
G	0004070	molecular_function	aspartate carbamoyltransferase activity
G	0004088	molecular_function	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
G	0006221	biological_process	pyrimidine nucleotide biosynthetic process
G	0006520	biological_process	amino acid metabolic process
G	0006541	biological_process	glutamine metabolic process
G	0009347	cellular_component	aspartate carbamoyltransferase complex
G	0016597	molecular_function	amino acid binding
G	0016740	molecular_function	transferase activity
G	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
G	0042802	molecular_function	identical protein binding
G	0044205	biological_process	'de novo' UMP biosynthetic process
G	0070207	biological_process	protein homotrimerization
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
H	0006221	biological_process	pyrimidine nucleotide biosynthetic process
H	0008270	molecular_function	zinc ion binding
H	0009347	cellular_component	aspartate carbamoyltransferase complex
H	0046872	molecular_function	metal ion binding
I	0004070	molecular_function	aspartate carbamoyltransferase activity
I	0004088	molecular_function	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
I	0006221	biological_process	pyrimidine nucleotide biosynthetic process
I	0006520	biological_process	amino acid metabolic process
I	0006541	biological_process	glutamine metabolic process
I	0009347	cellular_component	aspartate carbamoyltransferase complex
I	0016597	molecular_function	amino acid binding
I	0016740	molecular_function	transferase activity
I	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
I	0042802	molecular_function	identical protein binding
I	0044205	biological_process	'de novo' UMP biosynthetic process
I	0070207	biological_process	protein homotrimerization
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
J	0006221	biological_process	pyrimidine nucleotide biosynthetic process
J	0008270	molecular_function	zinc ion binding
J	0009347	cellular_component	aspartate carbamoyltransferase complex
J	0046872	molecular_function	metal ion binding
K	0004070	molecular_function	aspartate carbamoyltransferase activity
K	0004088	molecular_function	carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
K	0006221	biological_process	pyrimidine nucleotide biosynthetic process
K	0006520	biological_process	amino acid metabolic process
K	0006541	biological_process	glutamine metabolic process
K	0009347	cellular_component	aspartate carbamoyltransferase complex
K	0016597	molecular_function	amino acid binding
K	0016740	molecular_function	transferase activity
K	0016743	molecular_function	carboxyl- or carbamoyltransferase activity
K	0042802	molecular_function	identical protein binding
K	0044205	biological_process	'de novo' UMP biosynthetic process
K	0070207	biological_process	protein homotrimerization
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
L	0006221	biological_process	pyrimidine nucleotide biosynthetic process
L	0008270	molecular_function	zinc ion binding
L	0009347	cellular_component	aspartate carbamoyltransferase complex
L	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 201

Chain	Residue
D	CYS109
D	CYS114
D	CYS138
D	CYS141

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 201

Chain	Residue
B	CYS109
B	CYS114
B	CYS138
B	CYS141

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN J 201

Chain	Residue
J	CYS114
J	CYS138
J	CYS141
J	CYS109

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN L 201

Chain	Residue
L	CYS109
L	CYS114
L	CYS138
L	CYS141

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN H 201

Chain	Residue
H	CYS109
H	CYS114
H	CYS138
H	CYS141

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN F 201

Chain	Residue
F	CYS109
F	CYS114
F	CYS138
F	CYS141

Functional Information from PROSITE/UniProt

site_id	PS00097
Number of Residues	8
Details	CARBAMOYLTRANSFERASE Aspartate and ornithine carbamoyltransferases signature. FfEaSTRT

Chain	Residue	Details
I	PHE48-THR55

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	BINDING:

Chain	Residue	Details
D	CYS109
J	CYS114
J	CYS138
J	CYS141
L	CYS109
L	CYS114
L	CYS138
L	CYS141
H	CYS109
H	CYS114
H	CYS138
D	CYS114
H	CYS141
F	CYS109
F	CYS114
F	CYS138
F	CYS141
C	HIS134
C	GLN137
C	LEU267
C	PRO268
A	ARG54
D	CYS138
A	THR55
A	ARG105
A	HIS134
A	GLN137
A	LEU267
A	PRO268
E	ARG54
E	THR55
E	ARG105
E	HIS134
D	CYS141
E	GLN137
E	LEU267
E	PRO268
B	CYS109
B	CYS114
B	CYS138
B	CYS141
J	CYS109

site_id	SWS_FT_FI2
Number of Residues	18
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00001, ECO:0000305\|PubMed:3380787

Chain	Residue	Details
I	LYS84
C	LYS84
C	ARG167
C	ARG229
A	LYS84
A	ARG167
A	ARG229
E	LYS84
E	ARG167
E	ARG229
I	ARG167
I	ARG229
K	LYS84
K	ARG167
K	ARG229
G	LYS84
G	ARG167
G	ARG229

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 405

Chain	Residue	Details
I	ARG54	electrostatic stabiliser
I	THR55	electrostatic stabiliser, increase electrophilicity
I	LYS84	proton shuttle (general acid/base)
I	ARG105	electrostatic stabiliser, increase electrophilicity
I	HIS134	electrostatic stabiliser, increase electrophilicity

site_id	MCSA2
Number of Residues	5
Details	M-CSA 405

Chain	Residue	Details
K	ARG54	electrostatic stabiliser
K	THR55	electrostatic stabiliser, increase electrophilicity
K	LYS84	proton shuttle (general acid/base)
K	ARG105	electrostatic stabiliser, increase electrophilicity
K	HIS134	electrostatic stabiliser, increase electrophilicity

site_id	MCSA3
Number of Residues	5
Details	M-CSA 405

Chain	Residue	Details
G	ARG54	electrostatic stabiliser
G	THR55	electrostatic stabiliser, increase electrophilicity
G	LYS84	proton shuttle (general acid/base)
G	ARG105	electrostatic stabiliser, increase electrophilicity
G	HIS134	electrostatic stabiliser, increase electrophilicity

site_id	MCSA4
Number of Residues	5
Details	M-CSA 405

Chain	Residue	Details
C	ARG54	electrostatic stabiliser
C	THR55	electrostatic stabiliser, increase electrophilicity
C	LYS84	proton shuttle (general acid/base)
C	ARG105	electrostatic stabiliser, increase electrophilicity
C	HIS134	electrostatic stabiliser, increase electrophilicity

site_id	MCSA5
Number of Residues	5
Details	M-CSA 405

Chain	Residue	Details
A	ARG54	electrostatic stabiliser
A	THR55	electrostatic stabiliser, increase electrophilicity
A	LYS84	proton shuttle (general acid/base)
A	ARG105	electrostatic stabiliser, increase electrophilicity
A	HIS134	electrostatic stabiliser, increase electrophilicity

site_id	MCSA6
Number of Residues	5
Details	M-CSA 405

Chain	Residue	Details
E	ARG54	electrostatic stabiliser
E	THR55	electrostatic stabiliser, increase electrophilicity
E	LYS84	proton shuttle (general acid/base)
E	ARG105	electrostatic stabiliser, increase electrophilicity
E	HIS134	electrostatic stabiliser, increase electrophilicity

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)