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4E2F

Crystal Structure of E. coli Aspartate Transcarbamoylase K164E/E239K Mutant in an intermediate state

Functional Information from GO Data
ChainGOidnamespacecontents
A0004070molecular_functionaspartate carbamoyltransferase activity
A0004088molecular_functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
A0005515molecular_functionprotein binding
A0005737cellular_componentcytoplasm
A0005829cellular_componentcytosol
A0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
A0006221biological_processpyrimidine nucleotide biosynthetic process
A0006520biological_processamino acid metabolic process
A0006541biological_processglutamine metabolic process
A0009347cellular_componentaspartate carbamoyltransferase complex
A0016597molecular_functionamino acid binding
A0016740molecular_functiontransferase activity
A0016743molecular_functioncarboxyl- or carbamoyltransferase activity
A0042802molecular_functionidentical protein binding
A0044205biological_process'de novo' UMP biosynthetic process
A0070207biological_processprotein homotrimerization
B0005515molecular_functionprotein binding
B0005737cellular_componentcytoplasm
B0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
B0006221biological_processpyrimidine nucleotide biosynthetic process
B0008270molecular_functionzinc ion binding
B0009347cellular_componentaspartate carbamoyltransferase complex
B0046872molecular_functionmetal ion binding
C0004070molecular_functionaspartate carbamoyltransferase activity
C0004088molecular_functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
C0005515molecular_functionprotein binding
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
C0006221biological_processpyrimidine nucleotide biosynthetic process
C0006520biological_processamino acid metabolic process
C0006541biological_processglutamine metabolic process
C0009347cellular_componentaspartate carbamoyltransferase complex
C0016597molecular_functionamino acid binding
C0016740molecular_functiontransferase activity
C0016743molecular_functioncarboxyl- or carbamoyltransferase activity
C0042802molecular_functionidentical protein binding
C0044205biological_process'de novo' UMP biosynthetic process
C0070207biological_processprotein homotrimerization
D0005515molecular_functionprotein binding
D0005737cellular_componentcytoplasm
D0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
D0006221biological_processpyrimidine nucleotide biosynthetic process
D0008270molecular_functionzinc ion binding
D0009347cellular_componentaspartate carbamoyltransferase complex
D0046872molecular_functionmetal ion binding
E0004070molecular_functionaspartate carbamoyltransferase activity
E0004088molecular_functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
E0005515molecular_functionprotein binding
E0005737cellular_componentcytoplasm
E0005829cellular_componentcytosol
E0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
E0006221biological_processpyrimidine nucleotide biosynthetic process
E0006520biological_processamino acid metabolic process
E0006541biological_processglutamine metabolic process
E0009347cellular_componentaspartate carbamoyltransferase complex
E0016597molecular_functionamino acid binding
E0016740molecular_functiontransferase activity
E0016743molecular_functioncarboxyl- or carbamoyltransferase activity
E0042802molecular_functionidentical protein binding
E0044205biological_process'de novo' UMP biosynthetic process
E0070207biological_processprotein homotrimerization
F0005515molecular_functionprotein binding
F0005737cellular_componentcytoplasm
F0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
F0006221biological_processpyrimidine nucleotide biosynthetic process
F0008270molecular_functionzinc ion binding
F0009347cellular_componentaspartate carbamoyltransferase complex
F0046872molecular_functionmetal ion binding
G0004070molecular_functionaspartate carbamoyltransferase activity
G0004088molecular_functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
G0005515molecular_functionprotein binding
G0005737cellular_componentcytoplasm
G0005829cellular_componentcytosol
G0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
G0006221biological_processpyrimidine nucleotide biosynthetic process
G0006520biological_processamino acid metabolic process
G0006541biological_processglutamine metabolic process
G0009347cellular_componentaspartate carbamoyltransferase complex
G0016597molecular_functionamino acid binding
G0016740molecular_functiontransferase activity
G0016743molecular_functioncarboxyl- or carbamoyltransferase activity
G0042802molecular_functionidentical protein binding
G0044205biological_process'de novo' UMP biosynthetic process
G0070207biological_processprotein homotrimerization
H0005515molecular_functionprotein binding
H0005737cellular_componentcytoplasm
H0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
H0006221biological_processpyrimidine nucleotide biosynthetic process
H0008270molecular_functionzinc ion binding
H0009347cellular_componentaspartate carbamoyltransferase complex
H0046872molecular_functionmetal ion binding
I0004070molecular_functionaspartate carbamoyltransferase activity
I0004088molecular_functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
I0005515molecular_functionprotein binding
I0005737cellular_componentcytoplasm
I0005829cellular_componentcytosol
I0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
I0006221biological_processpyrimidine nucleotide biosynthetic process
I0006520biological_processamino acid metabolic process
I0006541biological_processglutamine metabolic process
I0009347cellular_componentaspartate carbamoyltransferase complex
I0016597molecular_functionamino acid binding
I0016740molecular_functiontransferase activity
I0016743molecular_functioncarboxyl- or carbamoyltransferase activity
I0042802molecular_functionidentical protein binding
I0044205biological_process'de novo' UMP biosynthetic process
I0070207biological_processprotein homotrimerization
J0005515molecular_functionprotein binding
J0005737cellular_componentcytoplasm
J0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
J0006221biological_processpyrimidine nucleotide biosynthetic process
J0008270molecular_functionzinc ion binding
J0009347cellular_componentaspartate carbamoyltransferase complex
J0046872molecular_functionmetal ion binding
K0004070molecular_functionaspartate carbamoyltransferase activity
K0004088molecular_functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
K0005515molecular_functionprotein binding
K0005737cellular_componentcytoplasm
K0005829cellular_componentcytosol
K0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
K0006221biological_processpyrimidine nucleotide biosynthetic process
K0006520biological_processamino acid metabolic process
K0006541biological_processglutamine metabolic process
K0009347cellular_componentaspartate carbamoyltransferase complex
K0016597molecular_functionamino acid binding
K0016740molecular_functiontransferase activity
K0016743molecular_functioncarboxyl- or carbamoyltransferase activity
K0042802molecular_functionidentical protein binding
K0044205biological_process'de novo' UMP biosynthetic process
K0070207biological_processprotein homotrimerization
L0005515molecular_functionprotein binding
L0005737cellular_componentcytoplasm
L0006207biological_process'de novo' pyrimidine nucleobase biosynthetic process
L0006221biological_processpyrimidine nucleotide biosynthetic process
L0008270molecular_functionzinc ion binding
L0009347cellular_componentaspartate carbamoyltransferase complex
L0046872molecular_functionmetal ion binding
Functional Information from PDB Data
site_idAC1
Number of Residues4
DetailsBINDING SITE FOR RESIDUE ZN D 201
ChainResidue
DCYS109
DCYS114
DCYS138
DCYS141

site_idAC2
Number of Residues4
DetailsBINDING SITE FOR RESIDUE ZN B 201
ChainResidue
BCYS109
BCYS114
BCYS138
BCYS141

site_idAC3
Number of Residues4
DetailsBINDING SITE FOR RESIDUE ZN J 201
ChainResidue
JCYS114
JCYS138
JCYS141
JCYS109

site_idAC4
Number of Residues4
DetailsBINDING SITE FOR RESIDUE ZN L 201
ChainResidue
LCYS109
LCYS114
LCYS138
LCYS141

site_idAC5
Number of Residues4
DetailsBINDING SITE FOR RESIDUE ZN H 201
ChainResidue
HCYS109
HCYS114
HCYS138
HCYS141

site_idAC6
Number of Residues4
DetailsBINDING SITE FOR RESIDUE ZN F 201
ChainResidue
FCYS109
FCYS114
FCYS138
FCYS141

Functional Information from PROSITE/UniProt
site_idPS00097
Number of Residues8
DetailsCARBAMOYLTRANSFERASE Aspartate and ornithine carbamoyltransferases signature. FfEaSTRT
ChainResidueDetails
IPHE48-THR55

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues24
DetailsBINDING:
ChainResidueDetails
DCYS109
JCYS114
JCYS138
JCYS141
LCYS109
LCYS114
LCYS138
LCYS141
HCYS109
HCYS114
HCYS138
DCYS114
HCYS141
FCYS109
FCYS114
FCYS138
FCYS141
CHIS134
CGLN137
CLEU267
CPRO268
AARG54
DCYS138
ATHR55
AARG105
AHIS134
AGLN137
ALEU267
APRO268
EARG54
ETHR55
EARG105
EHIS134
DCYS141
EGLN137
ELEU267
EPRO268
BCYS109
BCYS114
BCYS138
BCYS141
JCYS109

site_idSWS_FT_FI2
Number of Residues18
DetailsBINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00001, ECO:0000305|PubMed:3380787
ChainResidueDetails
ILYS84
CLYS84
CARG167
CARG229
ALYS84
AARG167
AARG229
ELYS84
EARG167
EARG229
IARG167
IARG229
KLYS84
KARG167
KARG229
GLYS84
GARG167
GARG229

Catalytic Information from CSA
site_idMCSA1
Number of Residues5
DetailsM-CSA 405
ChainResidueDetails
IARG54electrostatic stabiliser
ITHR55electrostatic stabiliser, increase electrophilicity
ILYS84proton shuttle (general acid/base)
IARG105electrostatic stabiliser, increase electrophilicity
IHIS134electrostatic stabiliser, increase electrophilicity

site_idMCSA2
Number of Residues5
DetailsM-CSA 405
ChainResidueDetails
KARG54electrostatic stabiliser
KTHR55electrostatic stabiliser, increase electrophilicity
KLYS84proton shuttle (general acid/base)
KARG105electrostatic stabiliser, increase electrophilicity
KHIS134electrostatic stabiliser, increase electrophilicity

site_idMCSA3
Number of Residues5
DetailsM-CSA 405
ChainResidueDetails
GARG54electrostatic stabiliser
GTHR55electrostatic stabiliser, increase electrophilicity
GLYS84proton shuttle (general acid/base)
GARG105electrostatic stabiliser, increase electrophilicity
GHIS134electrostatic stabiliser, increase electrophilicity

site_idMCSA4
Number of Residues5
DetailsM-CSA 405
ChainResidueDetails
CARG54electrostatic stabiliser
CTHR55electrostatic stabiliser, increase electrophilicity
CLYS84proton shuttle (general acid/base)
CARG105electrostatic stabiliser, increase electrophilicity
CHIS134electrostatic stabiliser, increase electrophilicity

site_idMCSA5
Number of Residues5
DetailsM-CSA 405
ChainResidueDetails
AARG54electrostatic stabiliser
ATHR55electrostatic stabiliser, increase electrophilicity
ALYS84proton shuttle (general acid/base)
AARG105electrostatic stabiliser, increase electrophilicity
AHIS134electrostatic stabiliser, increase electrophilicity

site_idMCSA6
Number of Residues5
DetailsM-CSA 405
ChainResidueDetails
EARG54electrostatic stabiliser
ETHR55electrostatic stabiliser, increase electrophilicity
ELYS84proton shuttle (general acid/base)
EARG105electrostatic stabiliser, increase electrophilicity
EHIS134electrostatic stabiliser, increase electrophilicity

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PDB entries from 2024-07-17

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