4D79

Crystal structure of E. coli tRNA N6-threonylcarbamoyladenosine dehydratase, TcdA, in complex with ATP at 1.768 Angstroem resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
A	0016020	cellular_component	membrane
A	0016874	molecular_function	ligase activity
A	0030955	molecular_function	potassium ion binding
A	0031402	molecular_function	sodium ion binding
A	0042803	molecular_function	protein homodimerization activity
A	0061503	molecular_function	tRNA threonylcarbamoyladenosine dehydratase
A	0061504	biological_process	cyclic threonylcarbamoyladenosine biosynthetic process
B	0000166	molecular_function	nucleotide binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
B	0016020	cellular_component	membrane
B	0016874	molecular_function	ligase activity
B	0030955	molecular_function	potassium ion binding
B	0031402	molecular_function	sodium ion binding
B	0042803	molecular_function	protein homodimerization activity
B	0061503	molecular_function	tRNA threonylcarbamoyladenosine dehydratase
B	0061504	biological_process	cyclic threonylcarbamoyladenosine biosynthetic process
C	0000166	molecular_function	nucleotide binding
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
C	0016020	cellular_component	membrane
C	0016874	molecular_function	ligase activity
C	0030955	molecular_function	potassium ion binding
C	0031402	molecular_function	sodium ion binding
C	0042803	molecular_function	protein homodimerization activity
C	0061503	molecular_function	tRNA threonylcarbamoyladenosine dehydratase
C	0061504	biological_process	cyclic threonylcarbamoyladenosine biosynthetic process
D	0000166	molecular_function	nucleotide binding
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
D	0016020	cellular_component	membrane
D	0016874	molecular_function	ligase activity
D	0030955	molecular_function	potassium ion binding
D	0031402	molecular_function	sodium ion binding
D	0042803	molecular_function	protein homodimerization activity
D	0061503	molecular_function	tRNA threonylcarbamoyladenosine dehydratase
D	0061504	biological_process	cyclic threonylcarbamoyladenosine biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K A 301

Chain	Residue
A	GLN158
A	GLN174
A	SER206
A	GLU208

---

site_id	AC2
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ATP A 401

Chain	Residue
A	ASP61
A	ASP63
A	ASN69
A	ARG72
A	GLN73
A	LYS85
A	PHE108
A	VAL109
A	ASP130
A	SER131
A	PRO134
A	HOH2029
A	HOH2044
A	HOH2049
A	VAL36
A	GLY37
A	GLY39
A	GLY40
A	ILE60

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 501

Chain	Residue
A	HIS255
A	LYS258
A	HOH2099
A	HOH2123
B	ASP160
B	THR162

---

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 502

Chain	Residue
A	CYS142
A	ILE147
A	PRO148
A	LEU149
A	GLY200
A	ASP202
A	HOH2081
A	HOH2085

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE K B 301

Chain	Residue
B	GLN158
B	GLN174
B	SER206
B	GLU208
B	HOH2088

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA B 302

Chain	Residue
A	THR162
A	HOH2094
A	HOH2095
A	HOH2098
B	THR162
B	HOH2092

---

site_id	AC7
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP B 401

Chain	Residue
B	VAL36
B	GLY37
B	GLY39
B	GLY40
B	ILE60
B	ASP61
B	ASP63
B	ARG72
B	GLN73
B	LYS85
B	PHE108
B	VAL109
B	ALA128
B	ILE129
B	ASP130
B	PRO134
B	HOH2050
B	HOH2140

---

site_id	AC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL B 501

Chain	Residue
B	CYS142
B	ILE147
B	PRO148
B	LEU149
B	GLY200
B	VAL201
B	ASP202
B	GOL502
B	HOH2083

---

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL B 502

Chain	Residue
B	TYR124
B	LYS259
B	LYS263
B	GOL501

---

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE K C 301

Chain	Residue
C	GLN158
C	GLN174
C	SER206
C	GLU208

---

site_id	BC2
Number of Residues	21
Details	BINDING SITE FOR RESIDUE ATP C 401

Chain	Residue
C	PRO134
C	HOH2039
C	HOH2046
C	HOH2048
C	HOH2053
C	VAL36
C	GLY37
C	GLY39
C	GLY40
C	ILE60
C	ASP61
C	ASP63
C	ASN69
C	ARG72
C	GLN73
C	LYS85
C	PHE108
C	VAL109
C	ILE129
C	ASP130
C	SER131

---

site_id	BC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL C 501

Chain	Residue
C	CYS142
C	ILE147
C	PRO148
C	LEU149
C	GLY200
C	VAL201
C	ASP202
C	HOH2098

---

site_id	BC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL C 502

Chain	Residue
C	VAL166
C	HIS255
C	LYS258
C	HOH2114
C	HOH2144
D	ASP160
D	PRO161

---

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE K D 301

Chain	Residue
D	GLN158
D	GLN174
D	SER206
D	GLU208
D	LEU210

---

site_id	BC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE NA D 302

Chain	Residue
C	THR162
C	HOH2109
C	HOH2110
C	HOH2111
C	HOH2112
D	THR162
D	HOH2075

---

site_id	BC7
Number of Residues	21
Details	BINDING SITE FOR RESIDUE ATP D 401

Chain	Residue
B	LYS196
B	LYS198
B	HOH2110
D	VAL36
D	GLY37
D	GLY39
D	GLY40
D	ILE60
D	ASP61
D	ASP63
D	ASN69
D	ARG72
D	GLN73
D	LYS85
D	PHE108
D	VAL109
D	ILE129
D	ASP130
D	PRO134
D	HOH2037
D	HOH2040

---

site_id	BC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL D 501

Chain	Residue
D	CYS142
D	ILE147
D	PRO148
D	LEU149
D	GLY200
D	VAL201
D	ASP202
D	HOH2068

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	80
Details	Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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