Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004517 | molecular_function | nitric-oxide synthase activity |
| A | 0006809 | biological_process | nitric oxide biosynthetic process |
| B | 0004517 | molecular_function | nitric-oxide synthase activity |
| B | 0006809 | biological_process | nitric oxide biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE HEM A 500 |
| Chain | Residue |
| A | TRP178 |
| A | TYR475 |
| A | H4B600 |
| A | Q16800 |
| A | HOH2370 |
| A | HOH2372 |
| A | HOH2373 |
| A | CYS184 |
| A | SER226 |
| A | PHE353 |
| A | SER354 |
| A | TRP356 |
| A | MET358 |
| A | GLU361 |
| A | PHE473 |
| site_id | AC2 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE H4B A 600 |
| Chain | Residue |
| A | SER102 |
| A | ARG365 |
| A | ALA446 |
| A | TRP447 |
| A | HEM500 |
| A | HOH2049 |
| A | HOH2282 |
| A | HOH2349 |
| A | HOH2372 |
| A | HOH2374 |
| B | TRP445 |
| B | PHE460 |
| B | HIS461 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE Q16 A 800 |
| Chain | Residue |
| A | PHE105 |
| A | VAL336 |
| A | PHE353 |
| A | TRP356 |
| A | GLU361 |
| A | HEM500 |
| A | HOH2283 |
| A | HOH2374 |
| B | TRP74 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT A 861 |
| Chain | Residue |
| A | ARG250 |
| A | ASN366 |
| A | ARG372 |
| A | HOH2204 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE BTB A 870 |
| Chain | Residue |
| A | CYS382 |
| A | ASP384 |
| A | GD1490 |
| A | HOH2298 |
| A | HOH2299 |
| A | HOH2300 |
| A | HOH2375 |
| A | HOH2376 |
| A | HOH2377 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE BTB A 871 |
| Chain | Residue |
| A | GLU377 |
| A | HOH2295 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL A 880 |
| Chain | Residue |
| A | GLU167 |
| A | HOH2105 |
| A | HOH2378 |
| A | HOH2379 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 900 |
| Chain | Residue |
| A | CYS94 |
| A | CYS99 |
| B | CYS94 |
| B | CYS99 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT A 860 |
| Chain | Residue |
| A | TRP356 |
| A | VAL418 |
| A | SER426 |
| A | HOH2133 |
| A | HOH2327 |
| site_id | BC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GD A 1490 |
| Chain | Residue |
| A | BTB870 |
| A | HOH2299 |
| A | HOH2300 |
| A | HOH2375 |
| A | HOH2376 |
| A | HOH2377 |
| A | HOH2381 |
| site_id | BC2 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE HEM B 500 |
| Chain | Residue |
| B | TRP178 |
| B | CYS184 |
| B | SER226 |
| B | PHE353 |
| B | SER354 |
| B | TRP356 |
| B | GLU361 |
| B | TRP447 |
| B | PHE473 |
| B | TYR475 |
| B | H4B600 |
| B | Q16800 |
| B | HOH2285 |
| B | HOH2357 |
| B | HOH2358 |
| B | HOH2363 |
| B | HOH2364 |
| site_id | BC3 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE H4B B 600 |
| Chain | Residue |
| B | HOH2346 |
| B | HOH2363 |
| B | HOH2365 |
| A | TRP445 |
| A | PHE460 |
| A | HIS461 |
| A | HOH2358 |
| B | SER102 |
| B | ARG365 |
| B | ALA446 |
| B | TRP447 |
| B | HEM500 |
| B | HOH2042 |
| site_id | BC4 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE Q16 B 800 |
| Chain | Residue |
| B | PHE105 |
| B | VAL336 |
| B | PHE353 |
| B | TRP356 |
| B | GLU361 |
| B | HEM500 |
| B | HOH2202 |
| B | HOH2285 |
| B | HOH2358 |
| B | HOH2365 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT B 860 |
| Chain | Residue |
| B | GLY186 |
| B | TRP356 |
| B | SER426 |
| B | HOH2131 |
| B | HOH2328 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT B 861 |
| Chain | Residue |
| B | ARG250 |
| B | ASN366 |
| B | ARG372 |
| B | HOH2207 |
| B | HOH2367 |
| site_id | BC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE BTB B 870 |
| Chain | Residue |
| B | THR319 |
| B | GLU321 |
| B | GD1490 |
| B | HOH2269 |
| B | HOH2270 |
| B | HOH2368 |
| B | HOH2369 |
| B | HOH2370 |
| B | HOH2371 |
| site_id | BC8 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE BTB B 871 |
| site_id | BC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GD B 1490 |
| Chain | Residue |
| B | THR319 |
| B | GLU321 |
| B | BTB870 |
| B | HOH2269 |
| B | HOH2270 |
Functional Information from PROSITE/UniProt
| site_id | PS60001 |
| Number of Residues | 8 |
| Details | NOS Nitric oxide synthase (NOS) signature. RCVGRIqW |
| Chain | Residue | Details |
| A | ARG183-TRP190 | |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10074942, ECO:0000269|PubMed:12437348, ECO:0000269|PubMed:18849972, ECO:0000269|PubMed:25286850, ECO:0007744|PDB:1M9J, ECO:0007744|PDB:1M9K, ECO:0007744|PDB:1M9M, ECO:0007744|PDB:1M9Q, ECO:0007744|PDB:1M9R, ECO:0007744|PDB:3EAH, ECO:0007744|PDB:3NOS, ECO:0007744|PDB:4D1O, ECO:0007744|PDB:4D1P |
| Chain | Residue | Details |
| A | CYS94 | |
| A | CYS99 | |
| B | CYS94 | |
| B | CYS99 | |
| Chain | Residue | Details |
| A | SER102 | |
| A | TYR475 | |
| B | SER102 | |
| B | GLN247 | |
| B | TRP356 | |
| B | TYR357 | |
| B | GLU361 | |
| B | ASN366 | |
| B | ALA446 | |
| B | TRP447 | |
| B | PHE460 | |
| A | GLN247 | |
| B | TYR475 | |
| A | TRP356 | |
| A | TYR357 | |
| A | GLU361 | |
| A | ASN366 | |
| A | ALA446 | |
| A | TRP447 | |
| A | PHE460 | |
| Chain | Residue | Details |
| A | CYS184 | |
| B | CYS184 | |
| Chain | Residue | Details |
| A | ARG365 | |
| B | ARG365 | |
| Chain | Residue | Details |
| A | SER114 | |
| B | SER114 | |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 935 |
| Chain | Residue | Details |
| A | CYS184 | metal ligand |
| A | ARG187 | steric role |
| A | TRP356 | electrostatic stabiliser |
| A | GLU361 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 935 |
| Chain | Residue | Details |
| B | CYS184 | metal ligand |
| B | ARG187 | steric role |
| B | TRP356 | electrostatic stabiliser |
| B | GLU361 | electrostatic stabiliser |
