4CS0
Direct visualisation of strain-induced protein post-translational modification
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004068 | molecular_function | aspartate 1-decarboxylase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006523 | biological_process | alanine biosynthetic process |
| A | 0015940 | biological_process | pantothenate biosynthetic process |
| A | 0016540 | biological_process | protein autoprocessing |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016831 | molecular_function | carboxy-lyase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0015940 | biological_process | pantothenate biosynthetic process |
| B | 0016485 | biological_process | protein processing |
| B | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| B | 0031638 | biological_process | zymogen activation |
| B | 1905502 | molecular_function | acetyl-CoA binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE ACO B 1128 |
| Chain | Residue |
| A | ARG102 |
| B | ARG74 |
| B | ARG75 |
| B | GLY76 |
| B | VAL77 |
| B | GLY78 |
| B | GLN79 |
| B | VAL102 |
| B | GLU103 |
| B | VAL107 |
| B | MET108 |
| B | TRP23 |
| B | PHE111 |
| B | MG1129 |
| B | HOH2016 |
| B | HOH2021 |
| B | GLU25 |
| B | TYR26 |
| B | SER65 |
| B | LEU66 |
| B | ARG67 |
| B | VAL68 |
| B | ARG73 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MG B 1129 |
| Chain | Residue |
| B | THR72 |
| B | ARG73 |
| B | ARG74 |
| B | ARG75 |
| B | GLY76 |
| B | VAL77 |
| B | ACO1128 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SCN A 1127 |
| Chain | Residue |
| A | ALA25 |
| A | TRP47 |
| A | ARG54 |
| A | THR57 |
| A | ALA74 |
| A | ALA75 |
| A | ILE86 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Schiff-base intermediate with substrate; via pyruvic acid","evidences":[{"source":"PubMed","id":"9546220","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton donor"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Pyruvic acid (Ser)","evidences":[{"source":"PubMed","id":"9546220","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 126 |
| Details | Domain: {"description":"N-acetyltransferase","evidences":[{"source":"HAMAP-Rule","id":"MF_02018","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 15 |
| Details | Region: {"description":"Interaction with PanD","evidences":[{"source":"PubMed","id":"25910242","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 9 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_02018","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"25910242","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUN-2008","submissionDatabase":"PDB data bank","title":"Solution NMR structure of putative N-acetyl transferase YhhK from E. coli bound to coenzyme A.","authors":["Cort J.R.","Yee A.","Arrowsmith C.H.","Kennedy M.A."]}},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2014","submissionDatabase":"PDB data bank","title":"Direct visualisation of strain-induced protein post-translational modification.","authors":["Monteiro D.C.F.","Patel V.","Bartlett C.P.","Grant T.D.","Nozaki S.","Gowdy J.A.","Snell E.H.","Niki H.","Pearson A.R.","Webb M.E."]}},{"source":"PDB","id":"2K5T","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 409 |
| Chain | Residue | Details |
| A | LYS9 | electrostatic stabiliser |
| A | ALA25 | covalently attached, electrofuge, electrophile |
| A | TYR58 | activator, increase nucleophilicity, proton acceptor, proton donor |
