4CS0
Direct visualisation of strain-induced protein post-translational modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-12-03 |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | I 4 |
| Unit cell lengths | 86.270, 86.270, 80.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.690 - 2.100 |
| R-factor | 0.17523 |
| Rwork | 0.172 |
| R-free | 0.23688 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4azd |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.094 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.700 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.180 | 0.620 |
| Number of reflections | 17250 | |
| <I/σ(I)> | 5.7 | 1.8 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 20% (W/V) POLYETHYLENE GLYCOL (PEG) 3350, 0.1 M BIS-TRIS PROPANE PH 7.4, 0.2 M POTASSIUM THIOCYANATE |
