4CI0
Electron cryo-microscopy of F420-reducing NiFe hydrogenase Frh
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| A | 0016151 | molecular_function | nickel cation binding |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050454 | molecular_function | coenzyme F420 hydrogenase activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 0051536 | molecular_function | iron-sulfur cluster binding |
| B | 0016151 | molecular_function | nickel cation binding |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0050454 | molecular_function | coenzyme F420 hydrogenase activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0051536 | molecular_function | iron-sulfur cluster binding |
| B | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0016151 | molecular_function | nickel cation binding |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0050454 | molecular_function | coenzyme F420 hydrogenase activity |
| C | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| C | 0051536 | molecular_function | iron-sulfur cluster binding |
| C | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| C | 0052592 | molecular_function | oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE FE A 1387 |
| Chain | Residue |
| A | CYS66 |
| A | PRO350 |
| A | CYS383 |
| A | NI1388 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NI A 1388 |
| Chain | Residue |
| A | CYS63 |
| A | CYS66 |
| A | CYS380 |
| A | CYS383 |
| A | FE1387 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE FE2 A 1389 |
| Chain | Residue |
| A | GLU44 |
| A | ALA347 |
| A | HIS386 |
| site_id | AC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SF4 B 1274 |
| Chain | Residue |
| B | GLY56 |
| B | CYS57 |
| B | THR58 |
| B | GLY59 |
| B | ASP60 |
| B | GLY130 |
| B | SER131 |
| B | CYS132 |
| B | CYS171 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SF4 B 1275 |
| Chain | Residue |
| B | ALA229 |
| B | CYS230 |
| B | THR232 |
| B | LEU235 |
| B | CYS249 |
| B | ILE250 |
| B | CYS252 |
| B | GLY253 |
| B | CYS255 |
| site_id | AC6 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE SF4 B 1276 |
| Chain | Residue |
| B | CYS220 |
| B | ILE221 |
| B | GLY222 |
| B | CYS223 |
| B | GLY224 |
| B | THR225 |
| B | CYS226 |
| B | MET237 |
| B | PRO242 |
| B | CYS259 |
| B | PRO260 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ZN B 1277 |
| Chain | Residue |
| B | CYS206 |
| B | CYS208 |
| site_id | AC8 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE SF4 C 1282 |
| Chain | Residue |
| B | ILE221 |
| C | ILE102 |
| C | PRO103 |
| C | CYS104 |
| C | CYS134 |
| C | MET135 |
| C | GLU136 |
| C | ASN137 |
| C | CYS192 |
| C | CYS195 |
| C | LYS261 |
| site_id | AC9 |
| Number of Residues | 29 |
| Details | BINDING SITE FOR RESIDUE FAD C 1283 |
| Chain | Residue |
| C | ALA23 |
| C | GLN24 |
| C | ASP25 |
| C | GLY26 |
| C | GLY27 |
| C | ILE28 |
| C | VAL29 |
| C | THR30 |
| C | VAL47 |
| C | ALA48 |
| C | ALA72 |
| C | GLY73 |
| C | THR74 |
| C | LYS75 |
| C | TYR76 |
| C | THR77 |
| C | ASN81 |
| C | ILE102 |
| C | GLN105 |
| C | ILE132 |
| C | TYR133 |
| C | CYS134 |
| C | MET135 |
| C | ASN137 |
| C | TYR198 |
| C | THR207 |
| C | GLY208 |
| C | SER209 |
| C | VAL210 |
Functional Information from PROSITE/UniProt
| site_id | PS00198 |
| Number of Residues | 12 |
| Details | 4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CiKCGiCYvQCP |
| Chain | Residue | Details |
| B | CYS249-PRO260 |
| site_id | PS00507 |
| Number of Residues | 26 |
| Details | NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEkmvtgkapetapvmvQRiCGVC |
| Chain | Residue | Details |
| A | ARG41-CYS66 |
| site_id | PS00508 |
| Number of Residues | 10 |
| Details | NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. YDPCLSCat.H |
| Chain | Residue | Details |
| A | TYR377-HIS386 |
