4CI0
Electron cryo-microscopy of F420-reducing NiFe hydrogenase Frh
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.2 |
| Detector | FEI FALCON II (4k x 4k) |
| Number of Images | 235 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.36 |
| Software | Coot,RELION |
| Symmetry | T |
