4CFH

Structure of an active form of mammalian AMPK

Replaces:  2Y94

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation
E	0000166	molecular_function	nucleotide binding
E	0004679	molecular_function	AMP-activated protein kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006110	biological_process	regulation of glycolytic process
E	0006629	biological_process	lipid metabolic process
E	0006631	biological_process	fatty acid metabolic process
E	0006633	biological_process	fatty acid biosynthetic process
E	0016208	molecular_function	AMP binding
E	0019887	molecular_function	protein kinase regulator activity
E	0019901	molecular_function	protein kinase binding
E	0031588	cellular_component	nucleotide-activated protein kinase complex
E	0031669	biological_process	cellular response to nutrient levels
E	0032991	cellular_component	protein-containing complex
E	0042149	biological_process	cellular response to glucose starvation
E	0043531	molecular_function	ADP binding
E	0044877	molecular_function	protein-containing complex binding
E	0045722	biological_process	positive regulation of gluconeogenesis
E	0050790	biological_process	regulation of catalytic activity
E	0051170	biological_process	import into nucleus

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE AMP E 1325

Chain	Residue
E	ARG69
E	VAL296
E	HIS297
E	ARG298
E	LYS169
E	ILE239
E	SER241
E	PHE243
E	ASP244
E	ARG268
E	VAL275
E	LEU276

---

site_id	AC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE AMP E 1326

Chain	Residue
E	HIS150
E	THR199
E	ILE203
E	ALA204
E	VAL224
E	SER225
E	ALA226
E	HIS297
E	ILE311
E	SER313
E	SER315
E	ASP316

---

site_id	AC3
Number of Residues	16
Details	BINDING SITE FOR RESIDUE STU A 1550

Chain	Residue
A	LEU22
A	GLY23
A	VAL24
A	GLY25
A	ALA43
A	LYS45
A	MET93
A	GLU94
A	TYR95
A	VAL96
A	GLY99
A	GLU100
A	GLU143
A	ASN144
A	LEU146
A	ASP157

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGVGTFGKVKvGkheltghk..........VAVK

Chain	Residue	Details
A	LEU22-LYS45

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VvHrDLKpeNVLL

Chain	Residue	Details
A	VAL135-LEU147

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	9
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"Q13131","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine; by LKB1 and CaMKK2","evidences":[{"source":"PubMed","id":"14511394","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14614828","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16054095","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16054096","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8910387","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"12764152","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q13131","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by ULK1","evidences":[{"source":"PubMed","id":"21460634","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine; by ULK1","evidences":[{"source":"PubMed","id":"21460634","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by ULK1","evidences":[{"source":"PubMed","id":"21460634","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	60
Details	Domain: {"description":"CBS 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00703","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	62
Details	Domain: {"description":"CBS 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00703","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	62
Details	Domain: {"description":"CBS 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU00703","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	21
Details	Motif: {"description":"AMPK pseudosubstrate"}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17851531","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2V92","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17851531","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21399626","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2V8Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2Y8L","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---