4BI5

CRYSTAL STRUCTURE OF A DOUBLE MUTANT (C202A AND C222D) OF TRIOSEPHOSPHATE ISOMERASE FROM GIARDIA LAMBLIA.

Replaces:  2YC8

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004807	molecular_function	triose-phosphate isomerase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006094	biological_process	gluconeogenesis
A	0006096	biological_process	glycolytic process
A	0008929	molecular_function	methylglyoxal synthase activity
A	0016829	molecular_function	lyase activity
A	0016853	molecular_function	isomerase activity
A	0019563	biological_process	glycerol catabolic process
A	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
B	0004807	molecular_function	triose-phosphate isomerase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006094	biological_process	gluconeogenesis
B	0006096	biological_process	glycolytic process
B	0008929	molecular_function	methylglyoxal synthase activity
B	0016829	molecular_function	lyase activity
B	0016853	molecular_function	isomerase activity
B	0019563	biological_process	glycerol catabolic process
B	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
C	0004807	molecular_function	triose-phosphate isomerase activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006094	biological_process	gluconeogenesis
C	0006096	biological_process	glycolytic process
C	0008929	molecular_function	methylglyoxal synthase activity
C	0016829	molecular_function	lyase activity
C	0016853	molecular_function	isomerase activity
C	0019563	biological_process	glycerol catabolic process
C	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
D	0004807	molecular_function	triose-phosphate isomerase activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006094	biological_process	gluconeogenesis
D	0006096	biological_process	glycolytic process
D	0008929	molecular_function	methylglyoxal synthase activity
D	0016829	molecular_function	lyase activity
D	0016853	molecular_function	isomerase activity
D	0019563	biological_process	glycerol catabolic process
D	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
E	0004807	molecular_function	triose-phosphate isomerase activity
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006094	biological_process	gluconeogenesis
E	0006096	biological_process	glycolytic process
E	0008929	molecular_function	methylglyoxal synthase activity
E	0016829	molecular_function	lyase activity
E	0016853	molecular_function	isomerase activity
E	0019563	biological_process	glycerol catabolic process
E	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
F	0004807	molecular_function	triose-phosphate isomerase activity
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006094	biological_process	gluconeogenesis
F	0006096	biological_process	glycolytic process
F	0008929	molecular_function	methylglyoxal synthase activity
F	0016829	molecular_function	lyase activity
F	0016853	molecular_function	isomerase activity
F	0019563	biological_process	glycerol catabolic process
F	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
G	0004807	molecular_function	triose-phosphate isomerase activity
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006094	biological_process	gluconeogenesis
G	0006096	biological_process	glycolytic process
G	0008929	molecular_function	methylglyoxal synthase activity
G	0016829	molecular_function	lyase activity
G	0016853	molecular_function	isomerase activity
G	0019563	biological_process	glycerol catabolic process
G	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
H	0004807	molecular_function	triose-phosphate isomerase activity
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006094	biological_process	gluconeogenesis
H	0006096	biological_process	glycolytic process
H	0008929	molecular_function	methylglyoxal synthase activity
H	0016829	molecular_function	lyase activity
H	0016853	molecular_function	isomerase activity
H	0019563	biological_process	glycerol catabolic process
H	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
I	0004807	molecular_function	triose-phosphate isomerase activity
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006094	biological_process	gluconeogenesis
I	0006096	biological_process	glycolytic process
I	0008929	molecular_function	methylglyoxal synthase activity
I	0016829	molecular_function	lyase activity
I	0016853	molecular_function	isomerase activity
I	0019563	biological_process	glycerol catabolic process
I	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
J	0004807	molecular_function	triose-phosphate isomerase activity
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006094	biological_process	gluconeogenesis
J	0006096	biological_process	glycolytic process
J	0008929	molecular_function	methylglyoxal synthase activity
J	0016829	molecular_function	lyase activity
J	0016853	molecular_function	isomerase activity
J	0019563	biological_process	glycerol catabolic process
J	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
K	0004807	molecular_function	triose-phosphate isomerase activity
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006094	biological_process	gluconeogenesis
K	0006096	biological_process	glycolytic process
K	0008929	molecular_function	methylglyoxal synthase activity
K	0016829	molecular_function	lyase activity
K	0016853	molecular_function	isomerase activity
K	0019563	biological_process	glycerol catabolic process
K	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
L	0004807	molecular_function	triose-phosphate isomerase activity
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006094	biological_process	gluconeogenesis
L	0006096	biological_process	glycolytic process
L	0008929	molecular_function	methylglyoxal synthase activity
L	0016829	molecular_function	lyase activity
L	0016853	molecular_function	isomerase activity
L	0019563	biological_process	glycerol catabolic process
L	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
M	0004807	molecular_function	triose-phosphate isomerase activity
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0006094	biological_process	gluconeogenesis
M	0006096	biological_process	glycolytic process
M	0008929	molecular_function	methylglyoxal synthase activity
M	0016829	molecular_function	lyase activity
M	0016853	molecular_function	isomerase activity
M	0019563	biological_process	glycerol catabolic process
M	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
N	0004807	molecular_function	triose-phosphate isomerase activity
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0006094	biological_process	gluconeogenesis
N	0006096	biological_process	glycolytic process
N	0008929	molecular_function	methylglyoxal synthase activity
N	0016829	molecular_function	lyase activity
N	0016853	molecular_function	isomerase activity
N	0019563	biological_process	glycerol catabolic process
N	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
O	0004807	molecular_function	triose-phosphate isomerase activity
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0006094	biological_process	gluconeogenesis
O	0006096	biological_process	glycolytic process
O	0008929	molecular_function	methylglyoxal synthase activity
O	0016829	molecular_function	lyase activity
O	0016853	molecular_function	isomerase activity
O	0019563	biological_process	glycerol catabolic process
O	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
P	0004807	molecular_function	triose-phosphate isomerase activity
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0006094	biological_process	gluconeogenesis
P	0006096	biological_process	glycolytic process
P	0008929	molecular_function	methylglyoxal synthase activity
P	0016829	molecular_function	lyase activity
P	0016853	molecular_function	isomerase activity
P	0019563	biological_process	glycerol catabolic process
P	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
Q	0004807	molecular_function	triose-phosphate isomerase activity
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0006094	biological_process	gluconeogenesis
Q	0006096	biological_process	glycolytic process
Q	0008929	molecular_function	methylglyoxal synthase activity
Q	0016829	molecular_function	lyase activity
Q	0016853	molecular_function	isomerase activity
Q	0019563	biological_process	glycerol catabolic process
Q	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
R	0004807	molecular_function	triose-phosphate isomerase activity
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006094	biological_process	gluconeogenesis
R	0006096	biological_process	glycolytic process
R	0008929	molecular_function	methylglyoxal synthase activity
R	0016829	molecular_function	lyase activity
R	0016853	molecular_function	isomerase activity
R	0019563	biological_process	glycerol catabolic process
R	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
S	0004807	molecular_function	triose-phosphate isomerase activity
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0006094	biological_process	gluconeogenesis
S	0006096	biological_process	glycolytic process
S	0008929	molecular_function	methylglyoxal synthase activity
S	0016829	molecular_function	lyase activity
S	0016853	molecular_function	isomerase activity
S	0019563	biological_process	glycerol catabolic process
S	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
T	0004807	molecular_function	triose-phosphate isomerase activity
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0006094	biological_process	gluconeogenesis
T	0006096	biological_process	glycolytic process
T	0008929	molecular_function	methylglyoxal synthase activity
T	0016829	molecular_function	lyase activity
T	0016853	molecular_function	isomerase activity
T	0019563	biological_process	glycerol catabolic process
T	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process

Functional Information from PROSITE/UniProt

site_id	PS00171
Number of Residues	11
Details	TIM_1 Triosephosphate isomerase active site. AYEPVWSIGTG

Chain	Residue	Details
A	ALA168-GLY178

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	ACT_SITE: Electrophile => ECO:0000255|PROSITE-ProRule:PRU10127

Chain	Residue	Details
A	HIS96
J	HIS96
K	HIS96
L	HIS96
M	HIS96
N	HIS96
O	HIS96
P	HIS96
Q	HIS96
R	HIS96
S	HIS96
B	HIS96
T	HIS96
C	HIS96
D	HIS96
E	HIS96
F	HIS96
G	HIS96
H	HIS96
I	HIS96

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU10127

Chain	Residue	Details
A	GLU170
J	GLU170
K	GLU170
L	GLU170
M	GLU170
N	GLU170
O	GLU170
P	GLU170
Q	GLU170
R	GLU170
S	GLU170
B	GLU170
T	GLU170
C	GLU170
D	GLU170
E	GLU170
F	GLU170
G	GLU170
H	GLU170
I	GLU170

---

site_id	SWS_FT_FI3
Number of Residues	40
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10127

Chain	Residue	Details
A	ASN11
E	LYS13
F	ASN11
F	LYS13
G	ASN11
G	LYS13
H	ASN11
H	LYS13
I	ASN11
I	LYS13
J	ASN11
A	LYS13
J	LYS13
K	ASN11
K	LYS13
L	ASN11
L	LYS13
M	ASN11
M	LYS13
N	ASN11
N	LYS13
O	ASN11
B	ASN11
O	LYS13
P	ASN11
P	LYS13
Q	ASN11
Q	LYS13
R	ASN11
R	LYS13
S	ASN11
S	LYS13
T	ASN11
B	LYS13
T	LYS13
C	ASN11
C	LYS13
D	ASN11
D	LYS13
E	ASN11

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