4BI5

CRYSTAL STRUCTURE OF A DOUBLE MUTANT (C202A AND C222D) OF TRIOSEPHOSPHATE ISOMERASE FROM GIARDIA LAMBLIA.

Replaces:  2YC8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	90
Detector technology	CCD
Collection date	2009-11-05
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	105.219, 131.566, 132.549
Unit cell angles	115.73, 89.81, 90.24

Refinement procedure

Resolution	78.870 - 2.700
R-factor	0.24098
Rwork	0.239
R-free	0.27232
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dp3
RMSD bond length	0.008
RMSD bond angle	0.947
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER (FOR MR)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.010	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.070	0.270
Number of reflections	147201
<I/σ(I)>	5.7	1.4
Completeness [%]	82.0	82
Redundancy	2.85	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		PROTEIN WAS CRYSTALLIZED FROM 0.05 M CESIUM CHLORIDE, 0.1 M MES MONOHYDRATE PH 6.5, 30% V/V JEFFAMINE M-600