4BFA
Crystal structure of E. coli dihydrouridine synthase C (DusC)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000049 | molecular_function | tRNA binding |
| A | 0002943 | biological_process | tRNA dihydrouridine synthesis |
| A | 0003723 | molecular_function | RNA binding |
| A | 0008033 | biological_process | tRNA processing |
| A | 0010181 | molecular_function | FMN binding |
| A | 0010467 | biological_process | gene expression |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0017150 | molecular_function | tRNA dihydrouridine synthase activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 0071461 | biological_process | cellular response to redox state |
| A | 0102262 | molecular_function | tRNA-dihydrouridine16 synthase activity |
| B | 0000049 | molecular_function | tRNA binding |
| B | 0002943 | biological_process | tRNA dihydrouridine synthesis |
| B | 0003723 | molecular_function | RNA binding |
| B | 0008033 | biological_process | tRNA processing |
| B | 0010181 | molecular_function | FMN binding |
| B | 0010467 | biological_process | gene expression |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0017150 | molecular_function | tRNA dihydrouridine synthase activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0071461 | biological_process | cellular response to redox state |
| B | 0102262 | molecular_function | tRNA-dihydrouridine16 synthase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE FMN A 1316 |
| Chain | Residue |
| A | ALA6 |
| A | HIS168 |
| A | TYR176 |
| A | ASN200 |
| A | GLY201 |
| A | GLU202 |
| A | GLY224 |
| A | ARG225 |
| A | HOH2132 |
| A | HOH2215 |
| A | HOH2219 |
| A | PRO7 |
| B | ARG177 |
| A | MET8 |
| A | GLU9 |
| A | PHE33 |
| A | GLN68 |
| A | ASN95 |
| A | CYS98 |
| A | LYS139 |
| site_id | AC2 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE FMN B 1316 |
| Chain | Residue |
| A | ARG177 |
| B | ALA6 |
| B | PRO7 |
| B | MET8 |
| B | GLU9 |
| B | PHE33 |
| B | GLN68 |
| B | ASN95 |
| B | LYS139 |
| B | HIS168 |
| B | TYR176 |
| B | ASN200 |
| B | GLY201 |
| B | GLU202 |
| B | GLY224 |
| B | ARG225 |
| B | HOH2132 |
| B | HOH2196 |
| B | HOH2199 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO A 1317 |
| Chain | Residue |
| A | TRP183 |
| A | GLN211 |
| A | HOH2196 |
| A | HOH2216 |
| A | HOH2303 |
| B | ARG281 |
| B | THR288 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE EDO A 1318 |
| Chain | Residue |
| A | ARG225 |
| A | ASN229 |
| A | TYR279 |
| A | LYS282 |
| A | HOH2236 |
| A | HOH2285 |
| B | GLU179 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO B 1317 |
| Chain | Residue |
| A | ALA132 |
| B | ARG2 |
| B | ASP27 |
| B | TRP90 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO B 1318 |
| Chain | Residue |
| B | ASP155 |
| B | GLN158 |
| B | GLN159 |
| B | HOH2306 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE NO3 A 1319 |
| Chain | Residue |
| A | ASP182 |
| A | TRP183 |
| A | GLN184 |
| A | HOH2303 |
| A | HOH2304 |
| B | ARG281 |
| B | PHE291 |
| B | GLN292 |
| B | ARG295 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE NO3 B 1319 |
| Chain | Residue |
| A | ARG281 |
| A | GLN292 |
| A | ARG295 |
| A | HOH2289 |
| B | ILE181 |
| B | ASP182 |
| B | TRP183 |
| B | GLN184 |
Functional Information from PROSITE/UniProt
| site_id | PS01136 |
| Number of Residues | 19 |
| Details | UPF0034 Uncharacterized protein family UPF0034 signature. VDLNcGCPskt.Vngsgg.GA |
| Chain | Residue | Details |
| A | VAL92-ALA110 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"UniProtKB","id":"Q5SMC7","evidenceCode":"ECO:0000250"},{"source":"PubMed","id":"25902496","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 14 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"23908023","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25902496","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3W9Z","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4BF9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4BFA","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | Site: {"description":"Interacts with tRNA; defines subfamily-specific binding signature","evidences":[{"source":"PubMed","id":"25902496","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | Site: {"description":"Interacts with tRNA","evidences":[{"source":"PubMed","id":"25902496","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
