4BFA
Crystal structure of E. coli dihydrouridine synthase C (DusC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.540, 99.953, 119.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.130 - 1.650 |
| R-factor | 0.13655 |
| Rwork | 0.134 |
| R-free | 0.17673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bf9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.278 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0024) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.100 | 0.710 |
| Number of reflections | 99677 | |
| <I/σ(I)> | 11 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | MOLECULAR DIMENSION MORPHEUS SCREEN CONDITION C10, pH 8.5 |
