4BEB

MUTANT (K220E) OF THE HSDR SUBUNIT OF THE ECOR124I RESTRICTION ENZYME IN COMPLEX WITH ATP

Replaces:  2Y3T

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0004519	molecular_function	endonuclease activity
A	0005524	molecular_function	ATP binding
A	0006304	biological_process	DNA modification
A	0009035	molecular_function	type I site-specific deoxyribonuclease activity
A	0009307	biological_process	DNA restriction-modification system
B	0003677	molecular_function	DNA binding
B	0004519	molecular_function	endonuclease activity
B	0005524	molecular_function	ATP binding
B	0006304	biological_process	DNA modification
B	0009035	molecular_function	type I site-specific deoxyribonuclease activity
B	0009307	biological_process	DNA restriction-modification system
C	0003677	molecular_function	DNA binding
C	0004519	molecular_function	endonuclease activity
C	0005524	molecular_function	ATP binding
C	0006304	biological_process	DNA modification
C	0009035	molecular_function	type I site-specific deoxyribonuclease activity
C	0009307	biological_process	DNA restriction-modification system
D	0003677	molecular_function	DNA binding
D	0004519	molecular_function	endonuclease activity
D	0005524	molecular_function	ATP binding
D	0006304	biological_process	DNA modification
D	0009035	molecular_function	type I site-specific deoxyribonuclease activity
D	0009307	biological_process	DNA restriction-modification system

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 1885

Chain	Residue
A	THR314
A	ASP408
A	ATP1886

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site_id	AC2
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ATP A 1886

Chain	Residue
A	THR309
A	GLY310
A	SER311
A	GLY312
A	LYS313
A	THR314
A	LEU315
A	THR661
A	GLY662
A	ASP664
A	ARG688
A	ARG691
A	MG1885
A	MET227
A	LEU270
A	VAL271
A	MET272
A	ARG273
A	GLN276

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG B 1885

Chain	Residue
B	THR314
B	ASP408
B	ATP1886

---

site_id	AC4
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP B 1886

Chain	Residue
B	LEU270
B	VAL271
B	MET272
B	ARG273
B	GLN276
B	THR309
B	GLY310
B	SER311
B	GLY312
B	LYS313
B	THR314
B	LEU315
B	THR661
B	GLY662
B	ASP664
B	ARG688
B	ARG691
B	MG1885

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG C 1885

Chain	Residue
C	THR314
C	ASP408
C	ATP1886

---

site_id	AC6
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ATP C 1886

Chain	Residue
C	MET227
C	LEU270
C	VAL271
C	MET272
C	ARG273
C	GLN276
C	THR309
C	GLY310
C	SER311
C	GLY312
C	LYS313
C	THR314
C	LEU315
C	THR661
C	GLY662
C	ASP664
C	ARG688
C	ARG691
C	MG1885

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG D 1885

Chain	Residue
D	THR314
D	ASP408
D	GLU409
D	ATP1886

---

site_id	AC8
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP D 1886

Chain	Residue
D	MET227
D	LEU270
D	VAL271
D	MET272
D	ARG273
D	GLN276
D	THR309
D	GLY310
D	SER311
D	GLY312
D	LYS313
D	THR314
D	THR661
D	GLY662
D	ASP664
D	ARG688
D	ARG691
D	MG1885

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00541

Chain	Residue	Details
A	HIS307
B	HIS307
C	HIS307
D	HIS307

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