Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4B1Z

Structure of the Phactr1 RPEL domain bound to G-actin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0001725	cellular_component	stress fiber
A	0003785	molecular_function	actin monomer binding
A	0005509	molecular_function	calcium ion binding
A	0005515	molecular_function	protein binding
A	0005523	molecular_function	tropomyosin binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005865	cellular_component	striated muscle thin filament
A	0005884	cellular_component	actin filament
A	0010628	biological_process	positive regulation of gene expression
A	0016787	molecular_function	hydrolase activity
A	0019904	molecular_function	protein domain specific binding
A	0030027	cellular_component	lamellipodium
A	0030041	biological_process	actin filament polymerization
A	0030175	cellular_component	filopodium
A	0030240	biological_process	skeletal muscle thin filament assembly
A	0031013	molecular_function	troponin I binding
A	0031432	molecular_function	titin binding
A	0031941	cellular_component	filamentous actin
A	0032036	molecular_function	myosin heavy chain binding
A	0032432	cellular_component	actin filament bundle
A	0042802	molecular_function	identical protein binding
A	0044297	cellular_component	cell body
A	0048306	molecular_function	calcium-dependent protein binding
A	0048741	biological_process	skeletal muscle fiber development
A	0051017	biological_process	actin filament bundle assembly
A	0090131	biological_process	mesenchyme migration
A	0098723	cellular_component	skeletal muscle myofibril
A	0140660	molecular_function	cytoskeletal motor activator activity
B	0000287	molecular_function	magnesium ion binding
B	0001725	cellular_component	stress fiber
B	0003785	molecular_function	actin monomer binding
B	0005509	molecular_function	calcium ion binding
B	0005515	molecular_function	protein binding
B	0005523	molecular_function	tropomyosin binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005865	cellular_component	striated muscle thin filament
B	0005884	cellular_component	actin filament
B	0010628	biological_process	positive regulation of gene expression
B	0016787	molecular_function	hydrolase activity
B	0019904	molecular_function	protein domain specific binding
B	0030027	cellular_component	lamellipodium
B	0030041	biological_process	actin filament polymerization
B	0030175	cellular_component	filopodium
B	0030240	biological_process	skeletal muscle thin filament assembly
B	0031013	molecular_function	troponin I binding
B	0031432	molecular_function	titin binding
B	0031941	cellular_component	filamentous actin
B	0032036	molecular_function	myosin heavy chain binding
B	0032432	cellular_component	actin filament bundle
B	0042802	molecular_function	identical protein binding
B	0044297	cellular_component	cell body
B	0048306	molecular_function	calcium-dependent protein binding
B	0048741	biological_process	skeletal muscle fiber development
B	0051017	biological_process	actin filament bundle assembly
B	0090131	biological_process	mesenchyme migration
B	0098723	cellular_component	skeletal muscle myofibril
B	0140660	molecular_function	cytoskeletal motor activator activity
C	0000287	molecular_function	magnesium ion binding
C	0001725	cellular_component	stress fiber
C	0003785	molecular_function	actin monomer binding
C	0005509	molecular_function	calcium ion binding
C	0005515	molecular_function	protein binding
C	0005523	molecular_function	tropomyosin binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005865	cellular_component	striated muscle thin filament
C	0005884	cellular_component	actin filament
C	0010628	biological_process	positive regulation of gene expression
C	0016787	molecular_function	hydrolase activity
C	0019904	molecular_function	protein domain specific binding
C	0030027	cellular_component	lamellipodium
C	0030041	biological_process	actin filament polymerization
C	0030175	cellular_component	filopodium
C	0030240	biological_process	skeletal muscle thin filament assembly
C	0031013	molecular_function	troponin I binding
C	0031432	molecular_function	titin binding
C	0031941	cellular_component	filamentous actin
C	0032036	molecular_function	myosin heavy chain binding
C	0032432	cellular_component	actin filament bundle
C	0042802	molecular_function	identical protein binding
C	0044297	cellular_component	cell body
C	0048306	molecular_function	calcium-dependent protein binding
C	0048741	biological_process	skeletal muscle fiber development
C	0051017	biological_process	actin filament bundle assembly
C	0090131	biological_process	mesenchyme migration
C	0098723	cellular_component	skeletal muscle myofibril
C	0140660	molecular_function	cytoskeletal motor activator activity
D	0000287	molecular_function	magnesium ion binding
D	0001725	cellular_component	stress fiber
D	0003785	molecular_function	actin monomer binding
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005523	molecular_function	tropomyosin binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005865	cellular_component	striated muscle thin filament
D	0005884	cellular_component	actin filament
D	0010628	biological_process	positive regulation of gene expression
D	0016787	molecular_function	hydrolase activity
D	0019904	molecular_function	protein domain specific binding
D	0030027	cellular_component	lamellipodium
D	0030041	biological_process	actin filament polymerization
D	0030175	cellular_component	filopodium
D	0030240	biological_process	skeletal muscle thin filament assembly
D	0031013	molecular_function	troponin I binding
D	0031432	molecular_function	titin binding
D	0031941	cellular_component	filamentous actin
D	0032036	molecular_function	myosin heavy chain binding
D	0032432	cellular_component	actin filament bundle
D	0042802	molecular_function	identical protein binding
D	0044297	cellular_component	cell body
D	0048306	molecular_function	calcium-dependent protein binding
D	0048741	biological_process	skeletal muscle fiber development
D	0051017	biological_process	actin filament bundle assembly
D	0090131	biological_process	mesenchyme migration
D	0098723	cellular_component	skeletal muscle myofibril
D	0140660	molecular_function	cytoskeletal motor activator activity
E	0000287	molecular_function	magnesium ion binding
E	0001725	cellular_component	stress fiber
E	0003785	molecular_function	actin monomer binding
E	0005509	molecular_function	calcium ion binding
E	0005515	molecular_function	protein binding
E	0005523	molecular_function	tropomyosin binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005865	cellular_component	striated muscle thin filament
E	0005884	cellular_component	actin filament
E	0010628	biological_process	positive regulation of gene expression
E	0016787	molecular_function	hydrolase activity
E	0019904	molecular_function	protein domain specific binding
E	0030027	cellular_component	lamellipodium
E	0030041	biological_process	actin filament polymerization
E	0030175	cellular_component	filopodium
E	0030240	biological_process	skeletal muscle thin filament assembly
E	0031013	molecular_function	troponin I binding
E	0031432	molecular_function	titin binding
E	0031941	cellular_component	filamentous actin
E	0032036	molecular_function	myosin heavy chain binding
E	0032432	cellular_component	actin filament bundle
E	0042802	molecular_function	identical protein binding
E	0044297	cellular_component	cell body
E	0048306	molecular_function	calcium-dependent protein binding
E	0048741	biological_process	skeletal muscle fiber development
E	0051017	biological_process	actin filament bundle assembly
E	0090131	biological_process	mesenchyme migration
E	0098723	cellular_component	skeletal muscle myofibril
E	0140660	molecular_function	cytoskeletal motor activator activity
F	0000287	molecular_function	magnesium ion binding
F	0001725	cellular_component	stress fiber
F	0003785	molecular_function	actin monomer binding
F	0005509	molecular_function	calcium ion binding
F	0005515	molecular_function	protein binding
F	0005523	molecular_function	tropomyosin binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005865	cellular_component	striated muscle thin filament
F	0005884	cellular_component	actin filament
F	0010628	biological_process	positive regulation of gene expression
F	0016787	molecular_function	hydrolase activity
F	0019904	molecular_function	protein domain specific binding
F	0030027	cellular_component	lamellipodium
F	0030041	biological_process	actin filament polymerization
F	0030175	cellular_component	filopodium
F	0030240	biological_process	skeletal muscle thin filament assembly
F	0031013	molecular_function	troponin I binding
F	0031432	molecular_function	titin binding
F	0031941	cellular_component	filamentous actin
F	0032036	molecular_function	myosin heavy chain binding
F	0032432	cellular_component	actin filament bundle
F	0042802	molecular_function	identical protein binding
F	0044297	cellular_component	cell body
F	0048306	molecular_function	calcium-dependent protein binding
F	0048741	biological_process	skeletal muscle fiber development
F	0051017	biological_process	actin filament bundle assembly
F	0090131	biological_process	mesenchyme migration
F	0098723	cellular_component	skeletal muscle myofibril
F	0140660	molecular_function	cytoskeletal motor activator activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP A 1376

Chain	Residue
A	GLY13
A	LYS213
A	GLU214
A	GLY301
A	GLY302
A	THR303
A	MET305
A	TYR306
A	LYS336
A	MG1377
A	SER14
A	GLY15
A	LEU16
A	LYS18
A	GLY156
A	ASP157
A	GLY158
A	VAL159

site_id	AC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG A 1377

Chain	Residue
A	ATP1376

site_id	AC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP B 1376

Chain	Residue
B	GLY13
B	SER14
B	GLY15
B	LEU16
B	LYS18
B	GLY156
B	ASP157
B	GLY158
B	VAL159
B	LYS213
B	GLU214
B	GLY301
B	GLY302
B	THR303
B	MET305
B	TYR306
B	LYS336
B	MG1377

site_id	AC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG B 1377

Chain	Residue
B	ATP1376

site_id	AC5
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP C 1376

Chain	Residue
C	GLY13
C	SER14
C	GLY15
C	LEU16
C	LYS18
C	GLY156
C	ASP157
C	GLY158
C	VAL159
C	LYS213
C	GLU214
C	GLY301
C	GLY302
C	THR303
C	MET305
C	TYR306
C	LYS336
C	MG1377

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG C 1377

Chain	Residue
C	ATP1376

site_id	AC7
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ATP D 1376

Chain	Residue
D	GLY13
D	SER14
D	GLY15
D	LEU16
D	LYS18
D	GLY156
D	ASP157
D	GLY158
D	VAL159
D	LYS213
D	GLU214
D	GLY301
D	GLY302
D	THR303
D	MET305
D	TYR306
D	MG1377

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG D 1377

Chain	Residue
D	GLN137
D	ATP1376

site_id	AC9
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ATP E 1376

Chain	Residue
E	GLY13
E	SER14
E	GLY15
E	LEU16
E	LYS18
E	GLY156
E	ASP157
E	GLY158
E	VAL159
E	LYS213
E	GLU214
E	GLY302
E	THR303
E	MET305
E	TYR306
E	LYS336
E	MG1377

site_id	BC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG E 1377

Chain	Residue
E	ASP154
E	ATP1376

site_id	BC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP F 1376

Chain	Residue
F	LYS18
F	GLY156
F	ASP157
F	GLY158
F	VAL159
F	ARG210
F	LYS213
F	GLU214
F	GLY301
F	GLY302
F	MET305
F	TYR306
F	LYS336
F	MG1377
F	GLY13
F	SER14
F	GLY15
F	LEU16

site_id	BC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG F 1377

Chain	Residue
F	ASP154
F	ATP1376

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL F 1378

Chain	Residue
F	TYR198
F	SER199
F	PHE200
F	GLU205
F	LEU242
M	GLN457

site_id	BC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 1378

Chain	Residue
A	TYR198
A	SER199
A	GLU205
A	LEU242
A	GLN246
M	SER505

site_id	BC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL C 1378

Chain	Residue
C	TYR198
C	SER199
C	PHE200
C	GLU205
C	LEU242
C	GLN246
C	GOL1379

site_id	BC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL B 1378

Chain	Residue
B	TYR198
B	SER199
B	PHE200
B	GLU205
B	LEU242
B	GLN246

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 1379

Chain	Residue
A	ALA310
A	ASP311
A	GLN314
A	ILE329

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL E 1378

Chain	Residue
E	TYR198
E	SER199
E	GLU205

site_id	CC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL N 1529

Chain	Residue
N	GLU527

site_id	CC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL C 1379

Chain	Residue
C	SER199
C	THR202
C	GLU205
C	GOL1378
N	THR463
N	SER467

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
A	TYR53-GLY63

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
A	TRP356-GLU364

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
A	LEU104-ARG116

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q9C0D0

Chain	Residue	Details
M	SER467
M	GLU436
N	SER467
N	GLU436
E	CYS0
F	CYS0

site_id	SWS_FT_FI2
Number of Residues	6
Details	MOD_RES: N-acetylaspartate; in Actin, alpha skeletal muscle => ECO:0000269\|PubMed:1150665

Chain	Residue	Details
A	ASP1
B	ASP1
C	ASP1
D	ASP1
E	ASP1
F	ASP1

site_id	SWS_FT_FI3
Number of Residues	12
Details	MOD_RES: Methionine (R)-sulfoxide => ECO:0000250\|UniProtKB:P68134

Chain	Residue	Details
A	MET44
E	MET47
F	MET44
F	MET47
A	MET47
B	MET44
B	MET47
C	MET44
C	MET47
D	MET44
D	MET47
E	MET44

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: N6-malonyllysine => ECO:0000250

Chain	Residue	Details
A	LYS61
B	LYS61
C	LYS61
D	LYS61
E	LYS61
F	LYS61

site_id	SWS_FT_FI5
Number of Residues	6
Details	MOD_RES: Tele-methylhistidine => ECO:0000269\|PubMed:1150665, ECO:0000269\|PubMed:16905096, ECO:0000269\|PubMed:213279, ECO:0000269\|PubMed:2395459, ECO:0000269\|PubMed:499690, ECO:0007744\|PDB:1ATN, ECO:0007744\|PDB:1NWK

Chain	Residue	Details
A	HIS73
B	HIS73
C	HIS73
D	HIS73
E	HIS73
F	HIS73

site_id	SWS_FT_FI6
Number of Residues	6
Details	MOD_RES: N6-methyllysine => ECO:0000250\|UniProtKB:P68133

Chain	Residue	Details
A	LYS84
B	LYS84
C	LYS84
D	LYS84
E	LYS84
F	LYS84

site_id	SWS_FT_FI7
Number of Residues	6
Details	MOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305\|PubMed:16905096

Chain	Residue	Details
A	ARG177
B	ARG177
C	ARG177
D	ARG177
E	ARG177
F	ARG177

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)