4B1Z

Structure of the Phactr1 RPEL domain bound to G-actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND
Synchrotron site	Diamond
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	127.060, 142.890, 184.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.618 - 3.300
R-factor	0.2145
Rwork	0.213
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.904
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.480
High resolution limit [Å]	3.300	3.300
Rmerge	0.140	0.590
Number of reflections	51065
<I/σ(I)>	4.6	4.6
Completeness [%]	99.6	100
Redundancy	5.6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1