Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4A8B

Symmetrized cryo-EM reconstruction of E. coli DegQ 12-mer in complex with lysozymes

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004175	molecular_function	endopeptidase activity
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0006508	biological_process	proteolysis
A	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0042597	cellular_component	periplasmic space
A	0042802	molecular_function	identical protein binding
A	0051603	biological_process	proteolysis involved in protein catabolic process
B	0004175	molecular_function	endopeptidase activity
B	0004252	molecular_function	serine-type endopeptidase activity
B	0005515	molecular_function	protein binding
B	0006508	biological_process	proteolysis
B	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
B	0008233	molecular_function	peptidase activity
B	0008236	molecular_function	serine-type peptidase activity
B	0042597	cellular_component	periplasmic space
B	0042802	molecular_function	identical protein binding
B	0051603	biological_process	proteolysis involved in protein catabolic process
C	0004175	molecular_function	endopeptidase activity
C	0004252	molecular_function	serine-type endopeptidase activity
C	0005515	molecular_function	protein binding
C	0006508	biological_process	proteolysis
C	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
C	0008233	molecular_function	peptidase activity
C	0008236	molecular_function	serine-type peptidase activity
C	0042597	cellular_component	periplasmic space
C	0042802	molecular_function	identical protein binding
C	0051603	biological_process	proteolysis involved in protein catabolic process
D	0004175	molecular_function	endopeptidase activity
D	0004252	molecular_function	serine-type endopeptidase activity
D	0005515	molecular_function	protein binding
D	0006508	biological_process	proteolysis
D	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
D	0008233	molecular_function	peptidase activity
D	0008236	molecular_function	serine-type peptidase activity
D	0042597	cellular_component	periplasmic space
D	0042802	molecular_function	identical protein binding
D	0051603	biological_process	proteolysis involved in protein catabolic process
E	0004175	molecular_function	endopeptidase activity
E	0004252	molecular_function	serine-type endopeptidase activity
E	0005515	molecular_function	protein binding
E	0006508	biological_process	proteolysis
E	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
E	0008233	molecular_function	peptidase activity
E	0008236	molecular_function	serine-type peptidase activity
E	0042597	cellular_component	periplasmic space
E	0042802	molecular_function	identical protein binding
E	0051603	biological_process	proteolysis involved in protein catabolic process
F	0004175	molecular_function	endopeptidase activity
F	0004252	molecular_function	serine-type endopeptidase activity
F	0005515	molecular_function	protein binding
F	0006508	biological_process	proteolysis
F	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
F	0008233	molecular_function	peptidase activity
F	0008236	molecular_function	serine-type peptidase activity
F	0042597	cellular_component	periplasmic space
F	0042802	molecular_function	identical protein binding
F	0051603	biological_process	proteolysis involved in protein catabolic process
G	0004175	molecular_function	endopeptidase activity
G	0004252	molecular_function	serine-type endopeptidase activity
G	0005515	molecular_function	protein binding
G	0006508	biological_process	proteolysis
G	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
G	0008233	molecular_function	peptidase activity
G	0008236	molecular_function	serine-type peptidase activity
G	0042597	cellular_component	periplasmic space
G	0042802	molecular_function	identical protein binding
G	0051603	biological_process	proteolysis involved in protein catabolic process
H	0004175	molecular_function	endopeptidase activity
H	0004252	molecular_function	serine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0006508	biological_process	proteolysis
H	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
H	0008233	molecular_function	peptidase activity
H	0008236	molecular_function	serine-type peptidase activity
H	0042597	cellular_component	periplasmic space
H	0042802	molecular_function	identical protein binding
H	0051603	biological_process	proteolysis involved in protein catabolic process
I	0004175	molecular_function	endopeptidase activity
I	0004252	molecular_function	serine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0006508	biological_process	proteolysis
I	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
I	0008233	molecular_function	peptidase activity
I	0008236	molecular_function	serine-type peptidase activity
I	0042597	cellular_component	periplasmic space
I	0042802	molecular_function	identical protein binding
I	0051603	biological_process	proteolysis involved in protein catabolic process
J	0004175	molecular_function	endopeptidase activity
J	0004252	molecular_function	serine-type endopeptidase activity
J	0005515	molecular_function	protein binding
J	0006508	biological_process	proteolysis
J	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
J	0008233	molecular_function	peptidase activity
J	0008236	molecular_function	serine-type peptidase activity
J	0042597	cellular_component	periplasmic space
J	0042802	molecular_function	identical protein binding
J	0051603	biological_process	proteolysis involved in protein catabolic process
K	0004175	molecular_function	endopeptidase activity
K	0004252	molecular_function	serine-type endopeptidase activity
K	0005515	molecular_function	protein binding
K	0006508	biological_process	proteolysis
K	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
K	0008233	molecular_function	peptidase activity
K	0008236	molecular_function	serine-type peptidase activity
K	0042597	cellular_component	periplasmic space
K	0042802	molecular_function	identical protein binding
K	0051603	biological_process	proteolysis involved in protein catabolic process
L	0004175	molecular_function	endopeptidase activity
L	0004252	molecular_function	serine-type endopeptidase activity
L	0005515	molecular_function	protein binding
L	0006508	biological_process	proteolysis
L	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
L	0008233	molecular_function	peptidase activity
L	0008236	molecular_function	serine-type peptidase activity
L	0042597	cellular_component	periplasmic space
L	0042802	molecular_function	identical protein binding
L	0051603	biological_process	proteolysis involved in protein catabolic process
M	0003796	molecular_function	lysozyme activity
M	0005515	molecular_function	protein binding
M	0005576	cellular_component	extracellular region
M	0005615	cellular_component	extracellular space
M	0005737	cellular_component	cytoplasm
M	0005783	cellular_component	endoplasmic reticulum
M	0016231	molecular_function	beta-N-acetylglucosaminidase activity
M	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
M	0016998	biological_process	cell wall macromolecule catabolic process
M	0031640	biological_process	killing of cells of another organism
M	0042742	biological_process	defense response to bacterium
M	0042802	molecular_function	identical protein binding
M	0050829	biological_process	defense response to Gram-negative bacterium
M	0050830	biological_process	defense response to Gram-positive bacterium
M	0051672	biological_process	obsolete catabolism by organism of cell wall peptidoglycan in other organism
N	0003796	molecular_function	lysozyme activity
N	0005515	molecular_function	protein binding
N	0005576	cellular_component	extracellular region
N	0005615	cellular_component	extracellular space
N	0005737	cellular_component	cytoplasm
N	0005783	cellular_component	endoplasmic reticulum
N	0016231	molecular_function	beta-N-acetylglucosaminidase activity
N	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
N	0016998	biological_process	cell wall macromolecule catabolic process
N	0031640	biological_process	killing of cells of another organism
N	0042742	biological_process	defense response to bacterium
N	0042802	molecular_function	identical protein binding
N	0050829	biological_process	defense response to Gram-negative bacterium
N	0050830	biological_process	defense response to Gram-positive bacterium
N	0051672	biological_process	obsolete catabolism by organism of cell wall peptidoglycan in other organism
O	0003796	molecular_function	lysozyme activity
O	0005515	molecular_function	protein binding
O	0005576	cellular_component	extracellular region
O	0005615	cellular_component	extracellular space
O	0005737	cellular_component	cytoplasm
O	0005783	cellular_component	endoplasmic reticulum
O	0016231	molecular_function	beta-N-acetylglucosaminidase activity
O	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
O	0016998	biological_process	cell wall macromolecule catabolic process
O	0031640	biological_process	killing of cells of another organism
O	0042742	biological_process	defense response to bacterium
O	0042802	molecular_function	identical protein binding
O	0050829	biological_process	defense response to Gram-negative bacterium
O	0050830	biological_process	defense response to Gram-positive bacterium
O	0051672	biological_process	obsolete catabolism by organism of cell wall peptidoglycan in other organism
P	0003796	molecular_function	lysozyme activity
P	0005515	molecular_function	protein binding
P	0005576	cellular_component	extracellular region
P	0005615	cellular_component	extracellular space
P	0005737	cellular_component	cytoplasm
P	0005783	cellular_component	endoplasmic reticulum
P	0016231	molecular_function	beta-N-acetylglucosaminidase activity
P	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
P	0016998	biological_process	cell wall macromolecule catabolic process
P	0031640	biological_process	killing of cells of another organism
P	0042742	biological_process	defense response to bacterium
P	0042802	molecular_function	identical protein binding
P	0050829	biological_process	defense response to Gram-negative bacterium
P	0050830	biological_process	defense response to Gram-positive bacterium
P	0051672	biological_process	obsolete catabolism by organism of cell wall peptidoglycan in other organism
Q	0003796	molecular_function	lysozyme activity
Q	0005515	molecular_function	protein binding
Q	0005576	cellular_component	extracellular region
Q	0005615	cellular_component	extracellular space
Q	0005737	cellular_component	cytoplasm
Q	0005783	cellular_component	endoplasmic reticulum
Q	0016231	molecular_function	beta-N-acetylglucosaminidase activity
Q	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
Q	0016998	biological_process	cell wall macromolecule catabolic process
Q	0031640	biological_process	killing of cells of another organism
Q	0042742	biological_process	defense response to bacterium
Q	0042802	molecular_function	identical protein binding
Q	0050829	biological_process	defense response to Gram-negative bacterium
Q	0050830	biological_process	defense response to Gram-positive bacterium
Q	0051672	biological_process	obsolete catabolism by organism of cell wall peptidoglycan in other organism
R	0003796	molecular_function	lysozyme activity
R	0005515	molecular_function	protein binding
R	0005576	cellular_component	extracellular region
R	0005615	cellular_component	extracellular space
R	0005737	cellular_component	cytoplasm
R	0005783	cellular_component	endoplasmic reticulum
R	0016231	molecular_function	beta-N-acetylglucosaminidase activity
R	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
R	0016998	biological_process	cell wall macromolecule catabolic process
R	0031640	biological_process	killing of cells of another organism
R	0042742	biological_process	defense response to bacterium
R	0042802	molecular_function	identical protein binding
R	0050829	biological_process	defense response to Gram-negative bacterium
R	0050830	biological_process	defense response to Gram-positive bacterium
R	0051672	biological_process	obsolete catabolism by organism of cell wall peptidoglycan in other organism

Functional Information from PROSITE/UniProt

site_id	PS00128
Number of Residues	19
Details	GLYCOSYL_HYDROL_F22_1 Glycosyl hydrolases family 22 (GH22) domain signature. CnipCsaLlssDItasvnC

Chain	Residue	Details
M	CYS76-CYS94

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE:

Chain	Residue	Details
M	GLU35
Q	ASP52
R	GLU35
R	ASP52
E	HIS82
E	ASP112
E	ALA187
F	HIS82
F	ASP112
F	ALA187
G	HIS82
M	ASP52
G	ASP112
G	ALA187
H	HIS82
H	ASP112
H	ALA187
I	HIS82
I	ASP112
I	ALA187
J	HIS82
J	ASP112
N	GLU35
J	ALA187
K	HIS82
K	ASP112
K	ALA187
L	HIS82
L	ASP112
L	ALA187
N	ASP52
O	GLU35
O	ASP52
P	GLU35
P	ASP52
Q	GLU35

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING:

Chain	Residue	Details
M	ASP101
D	GLU32
D	HIS82
D	ASP112
E	GLU32
E	HIS82
E	ASP112
F	GLU32
F	HIS82
F	ASP112
G	GLU32
N	ASP101
G	HIS82
G	ASP112
H	GLU32
H	HIS82
H	ASP112
I	GLU32
I	HIS82
I	ASP112
J	GLU32
J	HIS82
O	ASP101
J	ASP112
K	GLU32
K	HIS82
K	ASP112
L	GLU32
L	HIS82
L	ASP112
P	ASP101
Q	ASP101
R	ASP101
C	GLU32
C	HIS82
C	ASP112

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:21685389

Chain	Residue	Details
A	GLY185
J	GLY185
K	GLY185
L	GLY185
B	GLY185
C	GLY185
D	GLY185
E	GLY185
F	GLY185
G	GLY185
H	GLY185
I	GLY185

site_id	SWS_FT_FI4
Number of Residues	24
Details	BINDING:

Chain	Residue	Details
A	THR203
E	LEU242
F	THR203
F	LEU242
G	THR203
G	LEU242
H	THR203
H	LEU242
I	THR203
I	LEU242
J	THR203
A	LEU242
J	LEU242
K	THR203
K	LEU242
L	THR203
L	LEU242
B	THR203
B	LEU242
C	THR203
C	LEU242
D	THR203
D	LEU242
E	THR203

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 203

Chain	Residue	Details
M	GLU35	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
M	ASN46
M	ASP48
M	SER50
M	ASP52	covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, polar/non-polar interaction
M	ASN59

site_id	MCSA2
Number of Residues	6
Details	M-CSA 203

Chain	Residue	Details
N	GLU35	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
N	ASN46
N	ASP48
N	SER50
N	ASP52	covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, polar/non-polar interaction
N	ASN59

site_id	MCSA3
Number of Residues	6
Details	M-CSA 203

Chain	Residue	Details
O	GLU35	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
O	ASN46
O	ASP48
O	SER50
O	ASP52	covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, polar/non-polar interaction
O	ASN59

site_id	MCSA4
Number of Residues	6
Details	M-CSA 203

Chain	Residue	Details
P	GLU35	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
P	ASN46
P	ASP48
P	SER50
P	ASP52	covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, polar/non-polar interaction
P	ASN59

site_id	MCSA5
Number of Residues	6
Details	M-CSA 203

Chain	Residue	Details
Q	GLU35	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
Q	ASN46
Q	ASP48
Q	SER50
Q	ASP52	covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, polar/non-polar interaction
Q	ASN59

site_id	MCSA6
Number of Residues	6
Details	M-CSA 203

Chain	Residue	Details
R	GLU35	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
R	ASN46
R	ASP48
R	SER50
R	ASP52	covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, polar/non-polar interaction
R	ASN59

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)