4A8B
Symmetrized cryo-EM reconstruction of E. coli DegQ 12-mer in complex with lysozymes
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 100 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 13 |
| Software | Flex-EM,MODELLER,UCSF Chimera,IMAGIC,SPIDER |
| Symmetry | D2 |
