Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4A2U

CRP(CAP) from Myco. Tuberculosis, with cAMP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003677	molecular_function	DNA binding
A	0003700	molecular_function	DNA-binding transcription factor activity
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006355	biological_process	regulation of DNA-templated transcription
A	0009274	cellular_component	peptidoglycan-based cell wall
A	0030552	molecular_function	cAMP binding
A	0045892	biological_process	negative regulation of DNA-templated transcription
A	0045893	biological_process	positive regulation of DNA-templated transcription
B	0000166	molecular_function	nucleotide binding
B	0003677	molecular_function	DNA binding
B	0003700	molecular_function	DNA-binding transcription factor activity
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006355	biological_process	regulation of DNA-templated transcription
B	0009274	cellular_component	peptidoglycan-based cell wall
B	0030552	molecular_function	cAMP binding
B	0045892	biological_process	negative regulation of DNA-templated transcription
B	0045893	biological_process	positive regulation of DNA-templated transcription
C	0000166	molecular_function	nucleotide binding
C	0003677	molecular_function	DNA binding
C	0003700	molecular_function	DNA-binding transcription factor activity
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006355	biological_process	regulation of DNA-templated transcription
C	0009274	cellular_component	peptidoglycan-based cell wall
C	0030552	molecular_function	cAMP binding
C	0045892	biological_process	negative regulation of DNA-templated transcription
C	0045893	biological_process	positive regulation of DNA-templated transcription
D	0000166	molecular_function	nucleotide binding
D	0003677	molecular_function	DNA binding
D	0003700	molecular_function	DNA-binding transcription factor activity
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006355	biological_process	regulation of DNA-templated transcription
D	0009274	cellular_component	peptidoglycan-based cell wall
D	0030552	molecular_function	cAMP binding
D	0045892	biological_process	negative regulation of DNA-templated transcription
D	0045893	biological_process	positive regulation of DNA-templated transcription
E	0000166	molecular_function	nucleotide binding
E	0003677	molecular_function	DNA binding
E	0003700	molecular_function	DNA-binding transcription factor activity
E	0005515	molecular_function	protein binding
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006355	biological_process	regulation of DNA-templated transcription
E	0009274	cellular_component	peptidoglycan-based cell wall
E	0030552	molecular_function	cAMP binding
E	0045892	biological_process	negative regulation of DNA-templated transcription
E	0045893	biological_process	positive regulation of DNA-templated transcription
F	0000166	molecular_function	nucleotide binding
F	0003677	molecular_function	DNA binding
F	0003700	molecular_function	DNA-binding transcription factor activity
F	0005515	molecular_function	protein binding
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006355	biological_process	regulation of DNA-templated transcription
F	0009274	cellular_component	peptidoglycan-based cell wall
F	0030552	molecular_function	cAMP binding
F	0045892	biological_process	negative regulation of DNA-templated transcription
F	0045893	biological_process	positive regulation of DNA-templated transcription
G	0000166	molecular_function	nucleotide binding
G	0003677	molecular_function	DNA binding
G	0003700	molecular_function	DNA-binding transcription factor activity
G	0005515	molecular_function	protein binding
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0006355	biological_process	regulation of DNA-templated transcription
G	0009274	cellular_component	peptidoglycan-based cell wall
G	0030552	molecular_function	cAMP binding
G	0045892	biological_process	negative regulation of DNA-templated transcription
G	0045893	biological_process	positive regulation of DNA-templated transcription
H	0000166	molecular_function	nucleotide binding
H	0003677	molecular_function	DNA binding
H	0003700	molecular_function	DNA-binding transcription factor activity
H	0005515	molecular_function	protein binding
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0006355	biological_process	regulation of DNA-templated transcription
H	0009274	cellular_component	peptidoglycan-based cell wall
H	0030552	molecular_function	cAMP binding
H	0045892	biological_process	negative regulation of DNA-templated transcription
H	0045893	biological_process	positive regulation of DNA-templated transcription

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE CMP A 280

Chain	Residue
A	PHE38
A	ARG89
A	THR90
A	SER91
A	ARG130
A	THR134
B	LEU131
B	ASN135
A	ILE57
A	LEU69
A	MET72
A	PHE78
A	GLY79
A	GLU80
A	LEU81
A	SER82

site_id	AC2
Number of Residues	15
Details	BINDING SITE FOR RESIDUE CMP B 280

Chain	Residue
A	LEU131
A	ASN135
B	PHE38
B	ILE57
B	LEU69
B	PHE78
B	GLY79
B	GLU80
B	LEU81
B	SER82
B	ARG89
B	THR90
B	SER91
B	ARG130
B	THR134

site_id	AC3
Number of Residues	15
Details	BINDING SITE FOR RESIDUE CMP C 280

Chain	Residue
C	PHE38
C	ILE57
C	THR70
C	PHE78
C	GLY79
C	GLU80
C	LEU81
C	SER82
C	ARG89
C	THR90
C	SER91
C	ARG130
C	THR134
D	LEU131
D	ASN135

site_id	AC4
Number of Residues	14
Details	BINDING SITE FOR RESIDUE CMP D 280

Chain	Residue
C	LEU131
C	ASN135
D	PHE38
D	ILE57
D	PHE78
D	GLY79
D	GLU80
D	LEU81
D	SER82
D	ARG89
D	THR90
D	SER91
D	ARG130
D	THR134

site_id	AC5
Number of Residues	13
Details	BINDING SITE FOR RESIDUE CMP E 280

Chain	Residue
E	PHE38
E	ILE57
E	LEU69
E	PHE78
E	GLY79
E	GLU80
E	LEU81
E	SER82
E	ARG89
E	THR90
E	SER91
E	THR134
F	ASN135

site_id	AC6
Number of Residues	16
Details	BINDING SITE FOR RESIDUE CMP F 280

Chain	Residue
E	LEU131
E	ASN135
F	PHE38
F	ILE57
F	LEU69
F	THR70
F	MET72
F	PHE78
F	GLY79
F	GLU80
F	LEU81
F	SER82
F	ARG89
F	THR90
F	SER91
F	THR134

site_id	AC7
Number of Residues	14
Details	BINDING SITE FOR RESIDUE CMP G 280

Chain	Residue
G	SER91
G	ARG130
G	THR134
H	ASN135
G	PHE38
G	ILE57
G	LEU69
G	PHE78
G	GLY79
G	GLU80
G	LEU81
G	SER82
G	ARG89
G	THR90

site_id	AC8
Number of Residues	15
Details	BINDING SITE FOR RESIDUE CMP H 280

Chain	Residue
G	LEU131
G	ASN135
H	PHE38
H	ILE57
H	THR70
H	PHE78
H	GLY79
H	GLU80
H	LEU81
H	SER82
H	ARG89
H	THR90
H	SER91
H	ARG130
H	THR134

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	584
Details	Domain: {"description":"HTH crp-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00387","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	152
Details	DNA binding: {"description":"H-T-H motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU00387","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	136
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P0ACJ8","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	40
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAY-2010","submissionDatabase":"PDB data bank","title":"Crystal structure of cAMP receptor protein from Mycobacterium tuberculosis in complex with DNA and cAMP.","authors":["Akhter Y.","Pogenberg V.","Hasnain S.E.","Wilmanns M."]}}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)