4A2U
CRP(CAP) from Myco. Tuberculosis, with cAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.328, 67.801, 104.877 |
| Unit cell angles | 84.41, 83.46, 80.14 |
Refinement procedure
| Resolution | 16.000 - 2.630 |
| R-factor | 0.18853 |
| Rwork | 0.183 |
| R-free | 0.28767 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.663 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.750 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.080 | 0.550 |
| Number of reflections | 50716 | |
| <I/σ(I)> | 11.2 | 1.7 |
| Completeness [%] | 93.4 | 64.2 |
| Redundancy | 4.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 8 MG PER ML PROTEIN, 25 MM CAMP, 40 MM NA CL, 10% PEG 8K, 75 MM CA ACETATE, 50 MM NA MES., pH 5.8 |
