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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

3WKT

Complex structure of an open form of NADPH-cytochrome P450 reductase and heme oxygenase-1

Functional Information from GO Data
ChainGOidnamespacecontents
A0003958molecular_functionNADPH-hemoprotein reductase activity
A0004128molecular_functioncytochrome-b5 reductase activity, acting on NAD(P)H
A0005654cellular_componentnucleoplasm
A0005737cellular_componentcytoplasm
A0005789cellular_componentendoplasmic reticulum membrane
A0005829cellular_componentcytosol
A0006809biological_processnitric oxide biosynthetic process
A0007584biological_processresponse to nutrient
A0008047molecular_functionenzyme activator activity
A0008941molecular_functionnitric oxide dioxygenase NAD(P)H activity
A0009055molecular_functionelectron transfer activity
A0009410biological_processresponse to xenobiotic stimulus
A0009437biological_processcarnitine metabolic process
A0009725biological_processresponse to hormone
A0010181molecular_functionFMN binding
A0016491molecular_functionoxidoreductase activity
A0016787molecular_functionhydrolase activity
A0018393biological_processinternal peptidyl-lysine acetylation
A0019395biological_processfatty acid oxidation
A0019899molecular_functionenzyme binding
A0022900biological_processelectron transport chain
A0032332biological_processpositive regulation of chondrocyte differentiation
A0043066biological_processnegative regulation of apoptotic process
A0043602biological_processnitrate catabolic process
A0045880biological_processpositive regulation of smoothened signaling pathway
A0046210biological_processnitric oxide catabolic process
A0047726molecular_functioniron-cytochrome-c reductase activity
A0050660molecular_functionflavin adenine dinucleotide binding
A0050661molecular_functionNADP binding
A0060192biological_processnegative regulation of lipase activity
A0061913biological_processpositive regulation of growth plate cartilage chondrocyte proliferation
A0070988biological_processdemethylation
A0071371biological_processcellular response to gonadotropin stimulus
A0071372biological_processcellular response to follicle-stimulating hormone stimulus
A0071375biological_processcellular response to peptide hormone stimulus
A0071548biological_processresponse to dexamethasone
A0090031biological_processpositive regulation of steroid hormone biosynthetic process
A0090346biological_processorganofluorine metabolic process
B0003958molecular_functionNADPH-hemoprotein reductase activity
B0004128molecular_functioncytochrome-b5 reductase activity, acting on NAD(P)H
B0005654cellular_componentnucleoplasm
B0005737cellular_componentcytoplasm
B0005789cellular_componentendoplasmic reticulum membrane
B0005829cellular_componentcytosol
B0006809biological_processnitric oxide biosynthetic process
B0007584biological_processresponse to nutrient
B0008047molecular_functionenzyme activator activity
B0008941molecular_functionnitric oxide dioxygenase NAD(P)H activity
B0009055molecular_functionelectron transfer activity
B0009410biological_processresponse to xenobiotic stimulus
B0009437biological_processcarnitine metabolic process
B0009725biological_processresponse to hormone
B0010181molecular_functionFMN binding
B0016491molecular_functionoxidoreductase activity
B0016787molecular_functionhydrolase activity
B0018393biological_processinternal peptidyl-lysine acetylation
B0019395biological_processfatty acid oxidation
B0019899molecular_functionenzyme binding
B0022900biological_processelectron transport chain
B0032332biological_processpositive regulation of chondrocyte differentiation
B0043066biological_processnegative regulation of apoptotic process
B0043602biological_processnitrate catabolic process
B0045880biological_processpositive regulation of smoothened signaling pathway
B0046210biological_processnitric oxide catabolic process
B0047726molecular_functioniron-cytochrome-c reductase activity
B0050660molecular_functionflavin adenine dinucleotide binding
B0050661molecular_functionNADP binding
B0060192biological_processnegative regulation of lipase activity
B0061913biological_processpositive regulation of growth plate cartilage chondrocyte proliferation
B0070988biological_processdemethylation
B0071371biological_processcellular response to gonadotropin stimulus
B0071372biological_processcellular response to follicle-stimulating hormone stimulus
B0071375biological_processcellular response to peptide hormone stimulus
B0071548biological_processresponse to dexamethasone
B0090031biological_processpositive regulation of steroid hormone biosynthetic process
B0090346biological_processorganofluorine metabolic process
C0001525biological_processangiogenesis
C0001666biological_processresponse to hypoxia
C0002246biological_processwound healing involved in inflammatory response
C0004392molecular_functionheme oxygenase (decyclizing) activity
C0004630molecular_functionphospholipase D activity
C0005198molecular_functionstructural molecule activity
C0005515molecular_functionprotein binding
C0005634cellular_componentnucleus
C0005783cellular_componentendoplasmic reticulum
C0005789cellular_componentendoplasmic reticulum membrane
C0005829cellular_componentcytosol
C0005886cellular_componentplasma membrane
C0005901cellular_componentcaveola
C0006357biological_processregulation of transcription by RNA polymerase II
C0006644biological_processphospholipid metabolic process
C0006788biological_processheme oxidation
C0006879biological_processintracellular iron ion homeostasis
C0006915biological_processapoptotic process
C0006979biological_processresponse to oxidative stress
C0007264biological_processsmall GTPase-mediated signal transduction
C0008217biological_processregulation of blood pressure
C0008285biological_processnegative regulation of cell population proliferation
C0008630biological_processintrinsic apoptotic signaling pathway in response to DNA damage
C0009410biological_processresponse to xenobiotic stimulus
C0010656biological_processnegative regulation of muscle cell apoptotic process
C0016239biological_processpositive regulation of macroautophagy
C0016242biological_processnegative regulation of macroautophagy
C0016491molecular_functionoxidoreductase activity
C0019899molecular_functionenzyme binding
C0020037molecular_functionheme binding
C0031670biological_processcellular response to nutrient
C0032764biological_processnegative regulation of mast cell cytokine production
C0034101biological_processerythrocyte homeostasis
C0034605biological_processcellular response to heat
C0035094biological_processresponse to nicotine
C0035556biological_processintracellular signal transduction
C0042167biological_processheme catabolic process
C0042168biological_processheme metabolic process
C0042542biological_processresponse to hydrogen peroxide
C0042802molecular_functionidentical protein binding
C0042803molecular_functionprotein homodimerization activity
C0043065biological_processpositive regulation of apoptotic process
C0043305biological_processnegative regulation of mast cell degranulation
C0043524biological_processnegative regulation of neuron apoptotic process
C0043627biological_processresponse to estrogen
C0043922biological_processhost-mediated suppression of viral transcription
C0045766biological_processpositive regulation of angiogenesis
C0046872molecular_functionmetal ion binding
C0048471cellular_componentperinuclear region of cytoplasm
C0048661biological_processpositive regulation of smooth muscle cell proliferation
C0048662biological_processnegative regulation of smooth muscle cell proliferation
C0052869molecular_functionarachidonate omega-hydroxylase activity
C0060586biological_processmulticellular organismal-level iron ion homeostasis
C0071243biological_processcellular response to arsenic-containing substance
C0071276biological_processcellular response to cadmium ion
C0072719biological_processcellular response to cisplatin
C0090050biological_processpositive regulation of cell migration involved in sprouting angiogenesis
C0097421biological_processliver regeneration
C0110076biological_processnegative regulation of ferroptosis
C1900016biological_processnegative regulation of cytokine production involved in inflammatory response
C1902042biological_processnegative regulation of extrinsic apoptotic signaling pathway via death domain receptors
C1903589biological_processpositive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
C1903901biological_processnegative regulation of viral life cycle
C1904036biological_processnegative regulation of epithelial cell apoptotic process
C1904550biological_processresponse to arachidonate
C1904681biological_processresponse to 3-methylcholanthrene
C1904706biological_processnegative regulation of vascular associated smooth muscle cell proliferation
D0001525biological_processangiogenesis
D0001666biological_processresponse to hypoxia
D0002246biological_processwound healing involved in inflammatory response
D0004392molecular_functionheme oxygenase (decyclizing) activity
D0004630molecular_functionphospholipase D activity
D0005198molecular_functionstructural molecule activity
D0005515molecular_functionprotein binding
D0005634cellular_componentnucleus
D0005783cellular_componentendoplasmic reticulum
D0005789cellular_componentendoplasmic reticulum membrane
D0005829cellular_componentcytosol
D0005886cellular_componentplasma membrane
D0005901cellular_componentcaveola
D0006357biological_processregulation of transcription by RNA polymerase II
D0006644biological_processphospholipid metabolic process
D0006788biological_processheme oxidation
D0006879biological_processintracellular iron ion homeostasis
D0006915biological_processapoptotic process
D0006979biological_processresponse to oxidative stress
D0007264biological_processsmall GTPase-mediated signal transduction
D0008217biological_processregulation of blood pressure
D0008285biological_processnegative regulation of cell population proliferation
D0008630biological_processintrinsic apoptotic signaling pathway in response to DNA damage
D0009410biological_processresponse to xenobiotic stimulus
D0010656biological_processnegative regulation of muscle cell apoptotic process
D0016239biological_processpositive regulation of macroautophagy
D0016242biological_processnegative regulation of macroautophagy
D0016491molecular_functionoxidoreductase activity
D0019899molecular_functionenzyme binding
D0020037molecular_functionheme binding
D0031670biological_processcellular response to nutrient
D0032764biological_processnegative regulation of mast cell cytokine production
D0034101biological_processerythrocyte homeostasis
D0034605biological_processcellular response to heat
D0035094biological_processresponse to nicotine
D0035556biological_processintracellular signal transduction
D0042167biological_processheme catabolic process
D0042168biological_processheme metabolic process
D0042542biological_processresponse to hydrogen peroxide
D0042802molecular_functionidentical protein binding
D0042803molecular_functionprotein homodimerization activity
D0043065biological_processpositive regulation of apoptotic process
D0043305biological_processnegative regulation of mast cell degranulation
D0043524biological_processnegative regulation of neuron apoptotic process
D0043627biological_processresponse to estrogen
D0043922biological_processhost-mediated suppression of viral transcription
D0045766biological_processpositive regulation of angiogenesis
D0046872molecular_functionmetal ion binding
D0048471cellular_componentperinuclear region of cytoplasm
D0048661biological_processpositive regulation of smooth muscle cell proliferation
D0048662biological_processnegative regulation of smooth muscle cell proliferation
D0052869molecular_functionarachidonate omega-hydroxylase activity
D0060586biological_processmulticellular organismal-level iron ion homeostasis
D0071243biological_processcellular response to arsenic-containing substance
D0071276biological_processcellular response to cadmium ion
D0072719biological_processcellular response to cisplatin
D0090050biological_processpositive regulation of cell migration involved in sprouting angiogenesis
D0097421biological_processliver regeneration
D0110076biological_processnegative regulation of ferroptosis
D1900016biological_processnegative regulation of cytokine production involved in inflammatory response
D1902042biological_processnegative regulation of extrinsic apoptotic signaling pathway via death domain receptors
D1903589biological_processpositive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
D1903901biological_processnegative regulation of viral life cycle
D1904036biological_processnegative regulation of epithelial cell apoptotic process
D1904550biological_processresponse to arachidonate
D1904681biological_processresponse to 3-methylcholanthrene
D1904706biological_processnegative regulation of vascular associated smooth muscle cell proliferation
Functional Information from PDB Data
site_idAC1
Number of Residues16
DetailsBINDING SITE FOR RESIDUE FAD A 701
ChainResidue
AHIS315
AVAL472
ATYR474
AGLY484
AVAL485
AALA486
ATHR487
ATRP673
AARG420
AARG450
ATYR451
ATYR452
ASER453
ACYS468
AALA469
AVAL470
site_idAC2
Number of Residues19
DetailsBINDING SITE FOR RESIDUE FMN A 702
ChainResidue
ASER86
AGLN87
ATHR88
AGLY89
ATHR90
AALA91
AALA138
ATHR139
ATYR140
AGLY141
ALEU173
AGLY174
AASN175
ATYR178
AHIS180
APHE181
AASN182
AASP208
ALEU212
site_idAC3
Number of Residues13
DetailsBINDING SITE FOR RESIDUE NAP A 703
ChainResidue
AARG294
APRO529
AGLY530
ATHR531
ACYS562
AARG563
ASER592
AARG593
ALYS598
ATYR600
AGLN602
AASN631
AMET632
site_idAC4
Number of Residues18
DetailsBINDING SITE FOR RESIDUE FAD B 701
ChainResidue
BHIS315
BARG420
BARG450
BTYR451
BTYR452
BSER453
BCYS468
BALA469
BVAL470
BVAL472
BTYR474
BLYS477
BGLY484
BVAL485
BALA486
BTHR487
BALA534
BTRP673
site_idAC5
Number of Residues21
DetailsBINDING SITE FOR RESIDUE FMN B 702
ChainResidue
BSER86
BGLN87
BTHR88
BGLY89
BTHR90
BALA91
BALA138
BTHR139
BTYR140
BGLY141
BGLY143
BLEU173
BGLY174
BASN175
BTYR178
BHIS180
BPHE181
BASN182
BASP208
BLEU212
DVAL146
site_idAC6
Number of Residues11
DetailsBINDING SITE FOR RESIDUE NAP B 703
ChainResidue
BARG294
BVAL470
BTHR531
BCYS562
BSER592
BARG593
BLYS598
BTYR600
BVAL601
BGLN602
BMET632
site_idAC7
Number of Residues10
DetailsBINDING SITE FOR RESIDUE HEM C 300
ChainResidue
CGLN38
CTYR134
CTHR135
CARG136
CLEU138
CGLY139
CSER142
CLYS179
CARG183
CHIS25
site_idAC8
Number of Residues15
DetailsBINDING SITE FOR RESIDUE HEM D 300
ChainResidue
DLYS18
DHIS25
DGLU29
DMET34
DGLN38
DTYR134
DTHR135
DARG136
DGLY139
DSER142
DLYS179
DARG183
DPHE207
DASN210
DPHE214
Functional Information from PROSITE/UniProt
site_idPS00593
Number of Residues11
DetailsHEME_OXYGENASE Heme oxygenase signature. LVAHAYTRYLG
ChainResidueDetails
CLEU129-GLY139
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues144
DetailsDomain: {"description":"Flavodoxin-like","evidences":[{"source":"HAMAP-Rule","id":"MF_03212","evidenceCode":"ECO:0000255"}]}
ChainResidueDetails
site_idSWS_FT_FI2
Number of Residues62
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03212","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11371558","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19171935","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21345800","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9237990","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"OCT-2013","submissionDatabase":"PDB data bank","title":"Structural basis for the electron transfer from an open form of NADPH-cytochrome P450 oxidoreductase to heme oxygenase.","authors":["Sugishima M.","Sato H.","Higashimoto Y.","Harada J.","Wada K.","Fukuyama K.","Noguchi M."]}}]}
ChainResidueDetails
site_idSWS_FT_FI3
Number of Residues2
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03212","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11371558","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21345800","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9237990","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI4
Number of Residues4
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03212","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11371558","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19171935","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21345800","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9237990","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI5
Number of Residues2
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03212","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11371558","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19171935","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21345800","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"OCT-2013","submissionDatabase":"PDB data bank","title":"Structural basis for the electron transfer from an open form of NADPH-cytochrome P450 oxidoreductase to heme oxygenase.","authors":["Sugishima M.","Sato H.","Higashimoto Y.","Harada J.","Wada K.","Fukuyama K.","Noguchi M."]}}]}
ChainResidueDetails
site_idSWS_FT_FI6
Number of Residues4
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03212","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11371558","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19171935","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9237990","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"OCT-2013","submissionDatabase":"PDB data bank","title":"Structural basis for the electron transfer from an open form of NADPH-cytochrome P450 oxidoreductase to heme oxygenase.","authors":["Sugishima M.","Sato H.","Higashimoto Y.","Harada J.","Wada K.","Fukuyama K.","Noguchi M."]}}]}
ChainResidueDetails
site_idSWS_FT_FI7
Number of Residues6
DetailsBinding site: {"evidences":[{"source":"UniProtKB","id":"P09601","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI8
Number of Residues2
DetailsBinding site: {"description":"axial binding residue","evidences":[{"source":"UniProtKB","id":"P09601","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI9
Number of Residues2
DetailsSite: {"description":"Important for catalytic activity","evidences":[{"source":"UniProtKB","id":"P09601","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
Catalytic Information from CSA
site_idMCSA1
Number of Residues4
DetailsM-CSA 117
ChainResidueDetails
ASER453hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, radical stabiliser
ACYS626electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay, steric role, van der waals interaction
AASP671hydrogen bond acceptor, modifies pKa
ATRP673steric role
site_idMCSA2
Number of Residues4
DetailsM-CSA 117
ChainResidueDetails
BSER453hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, radical stabiliser
BCYS626electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay, steric role, van der waals interaction
BASP671hydrogen bond acceptor, modifies pKa
BTRP673steric role

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