3WIG

Human MEK1 kinase in complex with CH5126766 and MgAMP-PNP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE ANP A 401

Chain	Residue
A	LEU75
A	LYS193
A	SER195
A	ASN196
A	LEU198
A	ASP209
A	MG402
A	ALA77
A	GLY78
A	ALA96
A	LYS98
A	GLU145
A	MET147
A	SER151
A	GLN154

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 402

Chain	Residue
A	ASN196
A	ASP209
A	ANP401

---

site_id	AC3
Number of Residues	17
Details	BINDING SITE FOR RESIDUE CHU A 403

Chain	Residue
A	LEU119
A	VAL128
A	GLY129
A	ILE142
A	MET144
A	HIS189
A	ARG190
A	ASP191
A	ASP209
A	PHE210
A	GLY211
A	VAL212
A	SER213
A	LEU216
A	ILE217
A	MET220
A	ASN222

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PG4 A 404

Chain	Residue
A	GLY211
A	VAL212
A	GLY214
A	GLY214
A	GLN215

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A 405

Chain	Residue
A	ARG182
A	SER242
A	VAL243

---

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A 406

Chain	Residue
A	THR227

---

site_id	AC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A 407

Chain	Residue
A	PHE371

---

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL A 408

Chain	Residue
A	SER331
A	LEU332

---

site_id	AC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A 409

Chain	Residue
A	HIS240

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGAGNGGVVFkVshkpsglv..........MARK

Chain	Residue	Details
A	LEU75-LYS98

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKpsNILV

Chain	Residue	Details
A	ILE187-VAL199

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15543157","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17880056","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18951019","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19019675","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19706763","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21310613","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine; by BRAF and RAF1","evidences":[{"source":"PubMed","id":"10409742","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20956560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29433126","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8131746","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine; by BRAF and RAF1","evidences":[{"source":"PubMed","id":"20956560","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29433126","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8131746","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---