3W5O

Crystal Structure of Human DNA ligase IV

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003909	molecular_function	DNA ligase activity
A	0003910	molecular_function	DNA ligase (ATP) activity
A	0005524	molecular_function	ATP binding
A	0006281	biological_process	DNA repair
A	0006310	biological_process	DNA recombination
A	0071897	biological_process	DNA biosynthetic process
B	0003677	molecular_function	DNA binding
B	0003909	molecular_function	DNA ligase activity
B	0003910	molecular_function	DNA ligase (ATP) activity
B	0005524	molecular_function	ATP binding
B	0006281	biological_process	DNA repair
B	0006310	biological_process	DNA recombination
B	0071897	biological_process	DNA biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE ATP A 701

Chain	Residue
A	GLU271
A	MET430
A	LYS432
A	TRP447
A	LYS449
A	LYS451
A	HOH817
A	THR272
A	LYS273
A	LEU274
A	ASP275
A	ARG278
A	GLU331
A	PHE367
A	GLU427

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 702

Chain	Residue
A	ILE415
A	ASN419
A	ARG577

---

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 703

Chain	Residue
A	PRO528
A	HIS530

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 704

Chain	Residue
A	GLY276
A	ARG278
A	ARG293

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 705

Chain	Residue
A	SER292
A	ASN294
A	TYR296
A	TYR298

---

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 706

Chain	Residue
A	TYR298
A	GLN301

---

site_id	AC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 707

Chain	Residue
A	ARG32

---

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 708

Chain	Residue
A	THR381
A	ARG383

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 709

Chain	Residue
A	THR341
A	GLY474
A	SER475
A	MET479
A	MET480
A	CYS510

---

site_id	BC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 710

Chain	Residue
A	LYS544

---

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 711

Chain	Residue
A	ARG32
A	GLY197
A	VAL198
A	SER199
A	THR202

---

site_id	BC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 712

Chain	Residue
A	TRP590
A	HIS591

---

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 713

Chain	Residue
A	PHE529
A	HIS530
A	ARG531
A	LYS532

---

site_id	BC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 714

Chain	Residue
A	LYS162
A	LYS164
A	ASP165

---

site_id	BC6
Number of Residues	16
Details	BINDING SITE FOR RESIDUE ATP B 701

Chain	Residue
B	GLU271
B	THR272
B	LYS273
B	LEU274
B	ASP275
B	ARG278
B	GLU331
B	LYS345
B	PHE367
B	GLU427
B	MET430
B	LYS432
B	TRP447
B	LYS449
B	LYS451
B	HOH810

---

site_id	BC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 702

Chain	Residue
B	PRO528
B	HIS530

---

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 703

Chain	Residue
B	SER292
B	ARG293
B	ASN294
B	TYR298

---

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 704

Chain	Residue
B	ASN297
B	TYR298
B	ASP300
B	GLN301

---

site_id	CC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 705

Chain	Residue
B	ARG163

---

site_id	CC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 706

Chain	Residue
B	ASP165

---

site_id	CC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 707

Chain	Residue
B	GLN200

---

site_id	CC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 708

Chain	Residue
B	HIS530
B	ARG531
B	LYS532

---

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 709

Chain	Residue
B	LYS162
B	LYS164
B	ASP165

---

site_id	CC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 710

Chain	Residue
B	GLY276
B	GLU277
B	ARG278

---

site_id	CC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 711

Chain	Residue
B	ARG226
B	GLN227

---

site_id	CC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 712

Chain	Residue
B	SER536

---

Functional Information from PROSITE/UniProt

site_id	PS00333
Number of Residues	25
Details	DNA_LIGASE_A2 ATP-dependent DNA ligase signature 2. EGIMVKqplsi...YKpdk...RgegwlKIK

Chain	Residue	Details
A	GLU427-LYS451

---

site_id	PS00697
Number of Residues	9
Details	DNA_LIGASE_A1 ATP-dependent DNA ligase AMP-binding site. ETKLDGERM

Chain	Residue	Details
A	GLU271-MET279

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"N6-AMP-lysine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU10135","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23523427","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29980672","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3W1B","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P18858","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23523427","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3W1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3W5O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23523427","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3W5O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---