Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3W0L

The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001678	biological_process	intracellular glucose homeostasis
A	0003824	molecular_function	catalytic activity
A	0004340	molecular_function	glucokinase activity
A	0004396	molecular_function	hexokinase activity
A	0005524	molecular_function	ATP binding
A	0005536	molecular_function	D-glucose binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0005975	biological_process	carbohydrate metabolic process
A	0006006	biological_process	glucose metabolic process
A	0006007	biological_process	glucose catabolic process
A	0006096	biological_process	glycolytic process
A	0006163	biological_process	purine nucleotide metabolic process
A	0008865	molecular_function	fructokinase activity
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
A	0019158	molecular_function	mannokinase activity
A	0019318	biological_process	hexose metabolic process
A	0032024	biological_process	positive regulation of insulin secretion
A	0042593	biological_process	glucose homeostasis
A	0045721	biological_process	negative regulation of gluconeogenesis
A	0045725	biological_process	positive regulation of glycogen biosynthetic process
A	0046496	biological_process	nicotinamide nucleotide metabolic process
A	0046835	biological_process	carbohydrate phosphorylation
A	0050796	biological_process	regulation of insulin secretion
A	0051156	biological_process	glucose 6-phosphate metabolic process
B	0001678	biological_process	intracellular glucose homeostasis
B	0004857	molecular_function	enzyme inhibitor activity
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0009750	biological_process	response to fructose
B	0019210	molecular_function	kinase inhibitor activity
B	0019899	molecular_function	enzyme binding
B	0030246	molecular_function	carbohydrate binding
B	0033132	biological_process	negative regulation of glucokinase activity
B	0034504	biological_process	protein localization to nucleus
B	0042593	biological_process	glucose homeostasis
B	0070095	molecular_function	fructose-6-phosphate binding
B	0097367	molecular_function	carbohydrate derivative binding
B	0141089	molecular_function	glucose sensor activity
B	1901135	biological_process	carbohydrate derivative metabolic process
C	0000166	molecular_function	nucleotide binding
C	0001678	biological_process	intracellular glucose homeostasis
C	0003824	molecular_function	catalytic activity
C	0004340	molecular_function	glucokinase activity
C	0004396	molecular_function	hexokinase activity
C	0005524	molecular_function	ATP binding
C	0005536	molecular_function	D-glucose binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0005975	biological_process	carbohydrate metabolic process
C	0006006	biological_process	glucose metabolic process
C	0006007	biological_process	glucose catabolic process
C	0006096	biological_process	glycolytic process
C	0006163	biological_process	purine nucleotide metabolic process
C	0008865	molecular_function	fructokinase activity
C	0016301	molecular_function	kinase activity
C	0016740	molecular_function	transferase activity
C	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
C	0019158	molecular_function	mannokinase activity
C	0019318	biological_process	hexose metabolic process
C	0032024	biological_process	positive regulation of insulin secretion
C	0042593	biological_process	glucose homeostasis
C	0045721	biological_process	negative regulation of gluconeogenesis
C	0045725	biological_process	positive regulation of glycogen biosynthetic process
C	0046496	biological_process	nicotinamide nucleotide metabolic process
C	0046835	biological_process	carbohydrate phosphorylation
C	0050796	biological_process	regulation of insulin secretion
C	0051156	biological_process	glucose 6-phosphate metabolic process
D	0001678	biological_process	intracellular glucose homeostasis
D	0004857	molecular_function	enzyme inhibitor activity
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0009750	biological_process	response to fructose
D	0019210	molecular_function	kinase inhibitor activity
D	0019899	molecular_function	enzyme binding
D	0030246	molecular_function	carbohydrate binding
D	0033132	biological_process	negative regulation of glucokinase activity
D	0034504	biological_process	protein localization to nucleus
D	0042593	biological_process	glucose homeostasis
D	0070095	molecular_function	fructose-6-phosphate binding
D	0097367	molecular_function	carbohydrate derivative binding
D	0141089	molecular_function	glucose sensor activity
D	1901135	biological_process	carbohydrate derivative metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 501

Chain	Residue
A	GLY220
A	THR221
A	GLY403
A	SER404
A	ARG440

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NA A 502

Chain	Residue
A	MET231
A	VAL234
A	VAL237
A	GLY239

site_id	AC3
Number of Residues	13
Details	BINDING SITE FOR RESIDUE F6R B 701

Chain	Residue
B	CYS107
B	GLY108
B	THR109
B	SER110
B	GLU153
B	SER179
B	CYS180
B	SER183
B	SER258
B	ARG259
B	GLU347
B	LYS513
B	HOH811

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 B 702

Chain	Residue
B	LEU80
B	LYS81
B	GLY84
B	VAL286
B	THR287
B	GLU288
B	LEU291

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 703

Chain	Residue
B	ARG89
B	VAL93
B	LEU202

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 501

Chain	Residue
C	GLY220
C	THR221
C	SER404
C	ARG440

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA C 502

Chain	Residue
C	MET231
C	VAL234
C	VAL237
C	GLY239
C	HOH604

site_id	AC8
Number of Residues	12
Details	BINDING SITE FOR RESIDUE F6R D 701

Chain	Residue
D	CYS107
D	GLY108
D	THR109
D	GLU153
D	SER179
D	CYS180
D	GLY181
D	SER183
D	SER258
D	ARG259
D	GLU347
D	LYS513

Functional Information from PROSITE/UniProt

site_id	PS00378
Number of Residues	26
Details	HEXOKINASE_1 Hexokinase domain signature. LGFTFSFPvrhedIDkgiLlnWTKgF

Chain	Residue	Details
A	LEU139-PHE164

site_id	PS01272
Number of Residues	18
Details	GCKR Glucokinase regulatory protein family signature. GPEgVSGSSRMKGGSatK

Chain	Residue	Details
B	GLY250-LYS267

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI2
Number of Residues	358
Details	Domain: {"description":"SIS 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00797","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Region: {"description":"Essential for interaction with GCK","evidences":[{"source":"PubMed","id":"23733961","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23733961","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3W0L","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q14397","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)