3W0L
The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-22 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 109.318, 130.030, 175.117 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.478 - 2.920 |
R-factor | 0.2438 |
Rwork | 0.242 |
R-free | 0.27720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v4t 1nri |
RMSD bond length | 0.003 |
RMSD bond angle | 0.531 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 54157 | |
Completeness [%] | 99.4 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 0.005M MgSO4, 0.05M MES, 5% PEG4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |