3W0L

The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-22
Detector	OXFORD ONYX CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	109.318, 130.030, 175.117
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.478 - 2.920
R-factor	0.2438
Rwork	0.242
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v4t 1nri
RMSD bond length	0.003
RMSD bond angle	0.531
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	54157
Completeness [%]	99.4	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	290	0.005M MgSO4, 0.05M MES, 5% PEG4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K