3V38

Carboxypeptidase T mutant L254N

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004181	molecular_function	metallocarboxypeptidase activity
A	0006508	biological_process	proteolysis
A	0008270	molecular_function	zinc ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 401

Chain	Residue
A	HIS69
A	GLU72
A	HIS204
A	HOH544

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 402

Chain	Residue
A	GLU277
A	GOL404
A	GOL408
A	HOH544
A	HIS69
A	ARG129
A	ASN146
A	ARG147
A	TYR255

---

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SO4 A 403

Chain	Residue
A	TYR212
A	ASP220
A	ASP220
A	PHE230
A	LYS234
A	LYS234
A	HOH560
A	HOH560
A	HOH648
A	HOH648

---

site_id	AC4
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GOL A 404

Chain	Residue
A	ASN146
A	LEU211
A	ALA251
A	TYR255
A	THR257
A	ASP260
A	THR275
A	GLU277
A	SO4402
A	HOH580
A	HOH616
A	HOH633

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 405

Chain	Residue
A	GLY193
A	GLN195
A	HIS269
A	HOH685
A	HOH756

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 406

Chain	Residue
A	ARG71
A	LYS152
A	GLU166
A	TYR216
A	GOL409
A	GOL425

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 407

Chain	Residue
A	GLY137
A	SER138
A	SER139
A	TYR140
A	ARG171
A	SER172
A	HOH615

---

site_id	AC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL A 408

Chain	Residue
A	ARG129
A	THR205
A	TYR206
A	TYR255
A	GLU277
A	PHE287
A	SO4402
A	HOH507
A	HOH796
A	HOH797

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 409

Chain	Residue
A	ARG71
A	GLU166
A	GOL406
A	HOH551
A	HOH604
A	HOH801

---

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 410

Chain	Residue
A	PHE174
A	ARG183
A	ASN187
A	TRP264
A	GLN268
A	HOH640
A	HOH787

---

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 411

Chain	Residue
A	PRO3
A	SER4
A	TYR5
A	HOH708

---

site_id	BC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 412

Chain	Residue
A	LYS48
A	GLY54
A	THR55
A	ASP56
A	ARG189
A	GOL413
A	HOH653

---

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 413

Chain	Residue
A	LYS31
A	PHE33
A	TYR106
A	GOL412
A	HOH554

---

site_id	BC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 414

Chain	Residue
A	ASN98
A	ASN102
A	ARG103

---

site_id	BC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 415

Chain	Residue
A	LYS32
A	TRP45
A	HOH502
A	VAL16
A	ASN20
A	THR21
A	ASN25

---

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 416

Chain	Residue
A	TYR39
A	GLU40
A	HOH625
A	HOH692
A	HOH715

---

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 417

Chain	Residue
A	ASN28
A	LYS31
A	LYS32
A	PHE33
A	HOH773

---

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 418

Chain	Residue
A	ASN11
A	ASN13
A	GLU14
A	GOL419
A	HOH569

---

site_id	CC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 419

Chain	Residue
A	GLU14
A	ASN17
A	GOL418

---

site_id	CC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL A 420

Chain	Residue
A	ARG95
A	GLU303

---

site_id	CC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 421

Chain	Residue
A	THR239
A	TYR307
A	GLU310
A	LYS311
A	VAL318
A	HOH704
A	HOH799

---

site_id	CC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 422

Chain	Residue
A	ASP93
A	ARG95
A	LYS302

---

site_id	CC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 423

Chain	Residue
A	ARG132
A	HOH528
A	HOH619
A	HOH783

---

site_id	CC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 424

Chain	Residue
A	TYR12
A	ARG42
A	GLU117
A	HOH663
A	HOH776

---

site_id	CC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 425

Chain	Residue
A	SER127
A	LYS152
A	TYR216
A	PRO222
A	SER223
A	ASP224
A	GOL406
A	HOH545
A	HOH768

---

site_id	CC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 426

Chain	Residue
A	SER223
A	HOH588
A	HOH687
A	HOH724
A	HOH759
A	HOH769
A	HOH790

---

site_id	CC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL A 427

Chain	Residue
A	GLN249
A	ASN254

---

site_id	DC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 428

Chain	Residue
A	TRP128
A	TYR140
A	VAL141
A	HOH541
A	HOH584

---

site_id	DC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 429

Chain	Residue
A	TYR81
A	GLY295
A	SER299
A	HOH791

---

Functional Information from PROSITE/UniProt

site_id	PS00132
Number of Residues	23
Details	CARBOXYPEPT_ZN_1 Zinc carboxypeptidases, zinc-binding region 1 signature. PeVlYtaLhHArEhLTVemalyT

Chain	Residue	Details
A	PRO60-THR82

---

site_id	PS00133
Number of Residues	11
Details	CARBOXYPEPT_ZN_2 Zinc carboxypeptidases, zinc-binding region 2 signature. HTYSELIlYPY

Chain	Residue	Details
A	HIS204-TYR214

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	304
Details	Domain: {"description":"Peptidase M14","evidences":[{"source":"PROSITE-ProRule","id":"PRU01379","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU01379","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1521526","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01379","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1521526","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---