3V38
Carboxypeptidase T mutant L254N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 0.8 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 157.829, 157.829, 103.625 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.500 |
R-factor | 0.151 |
Rwork | 0.150 |
R-free | 0.16700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.133 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.500 |
Number of reflections | 119766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER DIFFUSION | 273 | COUNTER DIFFUSION, temperature 273K |