3V38

Carboxypeptidase T mutant L254N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	0.8
Spacegroup name	P 63 2 2
Unit cell lengths	157.829, 157.829, 103.625
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.500
R-factor	0.151
Rwork	0.150
R-free	0.16700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.133
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.500
Number of reflections	119766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER DIFFUSION		273	COUNTER DIFFUSION, temperature 273K