3V1S

Scaffold tailoring by a newly detected Pictet-Spenglerase ac-tivity of strictosidine synthase (STR1): from the common tryp-toline skeleton to the rare piperazino-indole framework

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005773	cellular_component	vacuole
A	0009820	biological_process	alkaloid metabolic process
A	0009821	biological_process	alkaloid biosynthetic process
A	0012505	cellular_component	endomembrane system
A	0016787	molecular_function	hydrolase activity
A	0016844	molecular_function	strictosidine synthase activity
A	0035834	biological_process	indole alkaloid metabolic process
B	0005773	cellular_component	vacuole
B	0009820	biological_process	alkaloid metabolic process
B	0009821	biological_process	alkaloid biosynthetic process
B	0012505	cellular_component	endomembrane system
B	0016787	molecular_function	hydrolase activity
B	0016844	molecular_function	strictosidine synthase activity
B	0035834	biological_process	indole alkaloid metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE 0LH A 401

Chain	Residue
A	TYR151
A	ILE179
A	VAL208
A	PHE226
A	GLU309
A	HOH520

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE 0LH B 401

Chain	Residue
B	GLU309
B	HOH562
B	TYR151
B	VAL167
B	VAL208

---

Functional Information from PROSITE/UniProt

site_id	PS00284
Number of Residues	11
Details	SERPIN Serpins signature. VSADSSFVLvA

Chain	Residue	Details
A	VAL214-ALA224

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PubMed","id":"16531499","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"18081287","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16531499","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2FPB","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16531499","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2FPC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00498","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
A	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---

site_id	MCSA2
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
B	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---