3UXJ

Crystal Structure of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with NADP and PreQ0

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006400	biological_process	tRNA modification
A	0008616	biological_process	tRNA queuosine(34) biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0033739	molecular_function	preQ1 synthase activity
A	0046857	molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006400	biological_process	tRNA modification
B	0008616	biological_process	tRNA queuosine(34) biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0033739	molecular_function	preQ1 synthase activity
B	0046857	molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006400	biological_process	tRNA modification
C	0008616	biological_process	tRNA queuosine(34) biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0033739	molecular_function	preQ1 synthase activity
C	0046857	molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006400	biological_process	tRNA modification
D	0008616	biological_process	tRNA queuosine(34) biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0033739	molecular_function	preQ1 synthase activity
D	0046857	molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PRF A 1194

Chain	Residue
A	LEU92
A	ILE267
A	NAP291
A	ILE93
A	GLU94
A	SER95
A	CYS194
A	ASP201
A	PHE232
A	HIS233
A	GLU234

---

site_id	AC2
Number of Residues	16
Details	BINDING SITE FOR RESIDUE NAP A 291

Chain	Residue
A	SER95
A	LYS96
A	LYS99
A	THR197
A	GLN199
A	LEU262
A	GLY263
A	HOH813
A	HOH996
A	PRF1194
B	LYS96
B	LYS99
B	LEU262
B	GLY263
B	HOH321
B	HOH1109

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 292

Chain	Residue
A	ALA208
A	TYR209
A	HIS210
A	SER252
A	HOH486
A	HOH1070
C	ALA111

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 293

Chain	Residue
A	CYS154
A	GLN158
A	ARG216
A	HOH1083

---

site_id	AC5
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PRF B 1194

Chain	Residue
B	TRP62
B	LEU92
B	ILE93
B	GLU94
B	SER95
B	CYS194
B	ASP201
B	PHE232
B	HIS233
B	GLU234
B	ILE267

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO B 291

Chain	Residue
B	ILE162
B	ALA163
B	ARG221
B	HOH317
B	HOH542

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO B 292

Chain	Residue
A	ASN140
B	THR149
B	HIS186
B	HIS188
B	HOH469
B	HOH991

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO B 293

Chain	Residue
B	GLN151
B	GLU206
B	HIS280
C	GLU145
C	PRO146

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO B 294

Chain	Residue
A	ASN164
B	GLY178
B	GLU179
B	LYS213
B	HOH746

---

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO B 295

Chain	Residue
B	TYR142
B	PRO146
B	GLN282
B	ARG283
B	HOH352
B	HOH388

---

site_id	BC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE PRF C 1194

Chain	Residue
C	TRP62
C	LEU92
C	ILE93
C	GLU94
C	SER95
C	CYS194
C	ILE196
C	ASP201
C	PHE232
C	HIS233
C	GLU234
C	ILE267

---

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO C 292

Chain	Residue
C	ILE162
C	ALA163
C	ARG221
C	HOH300
C	HOH637
C	HOH1059

---

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO C 293

Chain	Residue
B	GLU145
B	PRO146
C	GLN151
C	GLU206
C	HIS280

---

site_id	BC5
Number of Residues	15
Details	BINDING SITE FOR RESIDUE NAP D 291

Chain	Residue
C	LYS96
C	LYS99
C	LEU262
C	GLY263
C	HOH330
C	HOH1112
D	SER95
D	LYS96
D	LYS99
D	THR197
D	GLN199
D	LEU262
D	GLY263
D	HOH1021
D	PRF1194

---

site_id	BC6
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PRF D 1194

Chain	Residue
D	LEU92
D	ILE93
D	GLU94
D	SER95
D	CYS194
D	ASP201
D	PHE232
D	HIS233
D	GLU234
D	ILE267
D	NAP291

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Thioimide intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_00817","evidenceCode":"ECO:0000255"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JAN-2013","submissionDatabase":"PDB data bank","title":"Crystal structures of 7-cyano-7-deazaguanine reductase, QueF, from Vibrio cholerae.","authoringGroup":["Center for structural genomics of infectious diseases (CSGID)"]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Active site: {"description":"Proton donor","evidences":[{"source":"HAMAP-Rule","id":"MF_00817","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Binding site: {}

Chain

Residue

Details

---