3TNP

Structure and Allostery of the PKA RIIb Tetrameric Holoenzyme

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000166	molecular_function	nucleotide binding
B	0001932	biological_process	regulation of protein phosphorylation
B	0004862	molecular_function	cAMP-dependent protein kinase inhibitor activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005813	cellular_component	centrosome
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005952	cellular_component	cAMP-dependent protein kinase complex
B	0006631	biological_process	fatty acid metabolic process
B	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
B	0007612	biological_process	learning
B	0008603	molecular_function	cAMP-dependent protein kinase regulator activity
B	0019901	molecular_function	protein kinase binding
B	0019904	molecular_function	protein domain specific binding
B	0030425	cellular_component	dendrite
B	0030552	molecular_function	cAMP binding
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0034236	molecular_function	protein kinase A catalytic subunit binding
B	0043025	cellular_component	neuronal cell body
B	0043197	cellular_component	dendritic spine
B	0043198	cellular_component	dendritic shaft
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050804	biological_process	modulation of chemical synaptic transmission
B	0097332	biological_process	response to antipsychotic drug
B	0097546	cellular_component	ciliary base
B	0098794	cellular_component	postsynapse
B	0098978	cellular_component	glutamatergic synapse
B	0141162	biological_process	negative regulation of cAMP/PKA signal transduction
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0001669	cellular_component	acrosomal vesicle
C	0001707	biological_process	mesoderm formation
C	0001843	biological_process	neural tube closure
C	0004672	molecular_function	protein kinase activity
C	0004674	molecular_function	protein serine/threonine kinase activity
C	0004691	molecular_function	cAMP-dependent protein kinase activity
C	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005811	cellular_component	lipid droplet
C	0005813	cellular_component	centrosome
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005930	cellular_component	axoneme
C	0005952	cellular_component	cAMP-dependent protein kinase complex
C	0006338	biological_process	chromatin remodeling
C	0006397	biological_process	mRNA processing
C	0006468	biological_process	protein phosphorylation
C	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
C	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
C	0008284	biological_process	positive regulation of cell population proliferation
C	0010898	biological_process	positive regulation of triglyceride catabolic process
C	0016020	cellular_component	membrane
C	0016301	molecular_function	kinase activity
C	0016607	cellular_component	nuclear speck
C	0016740	molecular_function	transferase activity
C	0019901	molecular_function	protein kinase binding
C	0019904	molecular_function	protein domain specific binding
C	0030007	biological_process	intracellular potassium ion homeostasis
C	0030145	molecular_function	manganese ion binding
C	0031267	molecular_function	small GTPase binding
C	0031410	cellular_component	cytoplasmic vesicle
C	0031514	cellular_component	motile cilium
C	0031588	cellular_component	nucleotide-activated protein kinase complex
C	0031594	cellular_component	neuromuscular junction
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0031669	biological_process	cellular response to nutrient levels
C	0032991	cellular_component	protein-containing complex
C	0034237	molecular_function	protein kinase A regulatory subunit binding
C	0034605	biological_process	cellular response to heat
C	0036126	cellular_component	sperm flagellum
C	0038110	biological_process	interleukin-2-mediated signaling pathway
C	0038202	biological_process	TORC1 signaling
C	0043457	biological_process	regulation of cellular respiration
C	0044853	cellular_component	plasma membrane raft
C	0044877	molecular_function	protein-containing complex binding
C	0045542	biological_process	positive regulation of cholesterol biosynthetic process
C	0045667	biological_process	regulation of osteoblast differentiation
C	0045722	biological_process	positive regulation of gluconeogenesis
C	0045820	biological_process	negative regulation of glycolytic process
C	0045879	biological_process	negative regulation of smoothened signaling pathway
C	0046827	biological_process	positive regulation of protein export from nucleus
C	0048240	biological_process	sperm capacitation
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0048792	biological_process	spontaneous exocytosis of neurotransmitter
C	0050766	biological_process	positive regulation of phagocytosis
C	0050804	biological_process	modulation of chemical synaptic transmission
C	0051447	biological_process	negative regulation of meiotic cell cycle
C	0051726	biological_process	regulation of cell cycle
C	0051966	biological_process	regulation of synaptic transmission, glutamatergic
C	0061136	biological_process	regulation of proteasomal protein catabolic process
C	0070417	biological_process	cellular response to cold
C	0070613	biological_process	regulation of protein processing
C	0071333	biological_process	cellular response to glucose stimulus
C	0071374	biological_process	cellular response to parathyroid hormone stimulus
C	0071377	biological_process	cellular response to glucagon stimulus
C	0097225	cellular_component	sperm midpiece
C	0097546	cellular_component	ciliary base
C	0098793	cellular_component	presynapse
C	0098794	cellular_component	postsynapse
C	0098978	cellular_component	glutamatergic synapse
C	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
C	0106310	molecular_function	protein serine kinase activity
C	0140198	molecular_function	histone H1-4S35 kinase activity
C	0141156	biological_process	cAMP/PKA signal transduction
C	1904262	biological_process	negative regulation of TORC1 signaling
C	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
C	1990044	biological_process	protein localization to lipid droplet
C	2000810	biological_process	regulation of bicellular tight junction assembly
E	0000166	molecular_function	nucleotide binding
E	0001932	biological_process	regulation of protein phosphorylation
E	0004862	molecular_function	cAMP-dependent protein kinase inhibitor activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005813	cellular_component	centrosome
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005952	cellular_component	cAMP-dependent protein kinase complex
E	0006631	biological_process	fatty acid metabolic process
E	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
E	0007612	biological_process	learning
E	0008603	molecular_function	cAMP-dependent protein kinase regulator activity
E	0019901	molecular_function	protein kinase binding
E	0019904	molecular_function	protein domain specific binding
E	0030425	cellular_component	dendrite
E	0030552	molecular_function	cAMP binding
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0034236	molecular_function	protein kinase A catalytic subunit binding
E	0043025	cellular_component	neuronal cell body
E	0043197	cellular_component	dendritic spine
E	0043198	cellular_component	dendritic shaft
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0050804	biological_process	modulation of chemical synaptic transmission
E	0097332	biological_process	response to antipsychotic drug
E	0097546	cellular_component	ciliary base
E	0098794	cellular_component	postsynapse
E	0098978	cellular_component	glutamatergic synapse
E	0141162	biological_process	negative regulation of cAMP/PKA signal transduction
F	0000166	molecular_function	nucleotide binding
F	0000287	molecular_function	magnesium ion binding
F	0001669	cellular_component	acrosomal vesicle
F	0001707	biological_process	mesoderm formation
F	0001843	biological_process	neural tube closure
F	0004672	molecular_function	protein kinase activity
F	0004674	molecular_function	protein serine/threonine kinase activity
F	0004691	molecular_function	cAMP-dependent protein kinase activity
F	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005811	cellular_component	lipid droplet
F	0005813	cellular_component	centrosome
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0005930	cellular_component	axoneme
F	0005952	cellular_component	cAMP-dependent protein kinase complex
F	0006338	biological_process	chromatin remodeling
F	0006397	biological_process	mRNA processing
F	0006468	biological_process	protein phosphorylation
F	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
F	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
F	0008284	biological_process	positive regulation of cell population proliferation
F	0010898	biological_process	positive regulation of triglyceride catabolic process
F	0016020	cellular_component	membrane
F	0016301	molecular_function	kinase activity
F	0016607	cellular_component	nuclear speck
F	0016740	molecular_function	transferase activity
F	0019901	molecular_function	protein kinase binding
F	0019904	molecular_function	protein domain specific binding
F	0030007	biological_process	intracellular potassium ion homeostasis
F	0030145	molecular_function	manganese ion binding
F	0031267	molecular_function	small GTPase binding
F	0031410	cellular_component	cytoplasmic vesicle
F	0031514	cellular_component	motile cilium
F	0031588	cellular_component	nucleotide-activated protein kinase complex
F	0031594	cellular_component	neuromuscular junction
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0031669	biological_process	cellular response to nutrient levels
F	0032991	cellular_component	protein-containing complex
F	0034237	molecular_function	protein kinase A regulatory subunit binding
F	0034605	biological_process	cellular response to heat
F	0036126	cellular_component	sperm flagellum
F	0038110	biological_process	interleukin-2-mediated signaling pathway
F	0038202	biological_process	TORC1 signaling
F	0043457	biological_process	regulation of cellular respiration
F	0044853	cellular_component	plasma membrane raft
F	0044877	molecular_function	protein-containing complex binding
F	0045542	biological_process	positive regulation of cholesterol biosynthetic process
F	0045667	biological_process	regulation of osteoblast differentiation
F	0045722	biological_process	positive regulation of gluconeogenesis
F	0045820	biological_process	negative regulation of glycolytic process
F	0045879	biological_process	negative regulation of smoothened signaling pathway
F	0046827	biological_process	positive regulation of protein export from nucleus
F	0048240	biological_process	sperm capacitation
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0048792	biological_process	spontaneous exocytosis of neurotransmitter
F	0050766	biological_process	positive regulation of phagocytosis
F	0050804	biological_process	modulation of chemical synaptic transmission
F	0051447	biological_process	negative regulation of meiotic cell cycle
F	0051726	biological_process	regulation of cell cycle
F	0051966	biological_process	regulation of synaptic transmission, glutamatergic
F	0061136	biological_process	regulation of proteasomal protein catabolic process
F	0070417	biological_process	cellular response to cold
F	0070613	biological_process	regulation of protein processing
F	0071333	biological_process	cellular response to glucose stimulus
F	0071374	biological_process	cellular response to parathyroid hormone stimulus
F	0071377	biological_process	cellular response to glucagon stimulus
F	0097225	cellular_component	sperm midpiece
F	0097546	cellular_component	ciliary base
F	0098793	cellular_component	presynapse
F	0098794	cellular_component	postsynapse
F	0098978	cellular_component	glutamatergic synapse
F	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
F	0106310	molecular_function	protein serine kinase activity
F	0140198	molecular_function	histone H1-4S35 kinase activity
F	0141156	biological_process	cAMP/PKA signal transduction
F	1904262	biological_process	negative regulation of TORC1 signaling
F	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
F	1990044	biological_process	protein localization to lipid droplet
F	2000810	biological_process	regulation of bicellular tight junction assembly

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGTGSFGRVMlVkhkesgnh..........YAMK

Chain	Residue	Details
C	LEU49-LYS72

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. LiYrDLKpeNLLI

Chain	Residue	Details
C	LEU162-ILE174

---

site_id	PS00888
Number of Residues	17
Details	CNMP_BINDING_1 Cyclic nucleotide-binding domain signature 1. VIdQGDdGDnFYVIdrG

Chain	Residue	Details
B	VAL179-GLY195
B	ILE301-GLY317

---

site_id	PS00889
Number of Residues	18
Details	CNMP_BINDING_2 Cyclic nucleotide-binding domain signature 2. FGElALvtnkp......RAAsAhA

Chain	Residue	Details
B	PHE348-ALA365

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton acceptor"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	278
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P17612","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"22323819","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8395513","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine; by PDPK1","evidences":[{"source":"PubMed","id":"22323819","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8395513","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9707564","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"21866565","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"8395513","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"16452087","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"17242355","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19131326","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21183079","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---