3SLS

Crystal Structure of human MEK-1 kinase in complex with UCB1353770 and AMPPNP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation
B	0004672	molecular_function	protein kinase activity
B	0005524	molecular_function	ATP binding
B	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	28
Details	BINDING SITE FOR RESIDUE ANP A 400

Chain	Residue
A	HOH6
A	GLU144
A	MET146
A	SER150
A	GLN153
A	ASP190
A	LYS192
A	SER194
A	ASN195
A	LEU197
A	ASP208
A	ALA76
A	HOH358
A	HOH384
A	HOH386
A	MG401
A	HOH431
A	HOH432
A	HOH482
A	HOH495
A	77D500
A	GLY77
A	ASN78
A	GLY80
A	VAL82
A	ALA95
A	LYS97
A	MET143

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 401

Chain	Residue
A	LYS97
A	ASN195
A	ASP208
A	ANP400
A	HOH432

---

site_id	AC3
Number of Residues	13
Details	BINDING SITE FOR RESIDUE 77D A 500

Chain	Residue
A	HOH6
A	LYS97
A	ILE99
A	LEU115
A	VAL127
A	ILE141
A	MET143
A	CYS207
A	ASP208
A	PHE209
A	VAL211
A	SER212
A	ANP400

---

site_id	AC4
Number of Residues	29
Details	BINDING SITE FOR RESIDUE ANP B 400

Chain	Residue
B	HOH18
B	GLY75
B	ALA76
B	GLY77
B	ASN78
B	GLY80
B	VAL82
B	ALA95
B	LYS97
B	MET143
B	GLU144
B	MET146
B	SER150
B	GLN153
B	ASP190
B	LYS192
B	SER194
B	ASN195
B	LEU197
B	ASP208
B	HOH357
B	HOH382
B	MG401
B	HOH404
B	HOH456
B	HOH466
B	HOH493
B	HOH494
B	77D500

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 401

Chain	Residue
B	ASN195
B	ASP208
B	ANP400
B	HOH466

---

site_id	AC6
Number of Residues	17
Details	BINDING SITE FOR RESIDUE 77D B 500

Chain	Residue
B	ASN78
B	LYS97
B	ILE99
B	LEU115
B	VAL127
B	ILE141
B	MET143
B	CYS207
B	ASP208
B	PHE209
B	GLY210
B	VAL211
B	SER212
B	ANP400
B	HOH433
B	HOH450
B	HOH456

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGAGNGGVVFkVshkpsglv..........MARK

Chain	Residue	Details
A	LEU74-LYS97

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKpsNILV

Chain	Residue	Details
A	ILE186-VAL198

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15543157","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17880056","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18951019","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19019675","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19706763","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21310613","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19161339","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3EQF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---