3SEA
Structure of Rheb-Y35A mutant in GDP- and GMPPNP-bound forms
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000139 | cellular_component | Golgi membrane |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005681 | cellular_component | spliceosomal complex |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005764 | cellular_component | lysosome |
| A | 0005765 | cellular_component | lysosomal membrane |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005789 | cellular_component | endoplasmic reticulum membrane |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0007165 | biological_process | signal transduction |
| A | 0007264 | biological_process | small GTPase-mediated signal transduction |
| A | 0012505 | cellular_component | endomembrane system |
| A | 0014069 | cellular_component | postsynaptic density |
| A | 0016020 | cellular_component | membrane |
| A | 0016241 | biological_process | regulation of macroautophagy |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019003 | molecular_function | GDP binding |
| A | 0019901 | molecular_function | protein kinase binding |
| A | 0030295 | molecular_function | protein kinase activator activity |
| A | 0031669 | biological_process | cellular response to nutrient levels |
| A | 0032006 | biological_process | regulation of TOR signaling |
| A | 0032008 | biological_process | positive regulation of TOR signaling |
| A | 0043539 | molecular_function | protein serine/threonine kinase activator activity |
| A | 0045202 | cellular_component | synapse |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048709 | biological_process | oligodendrocyte differentiation |
| A | 0048714 | biological_process | positive regulation of oligodendrocyte differentiation |
| A | 0051726 | biological_process | regulation of cell cycle |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0120163 | biological_process | negative regulation of cold-induced thermogenesis |
| A | 1904263 | biological_process | positive regulation of TORC1 signaling |
| A | 2000074 | biological_process | regulation of type B pancreatic cell development |
| B | 0000139 | cellular_component | Golgi membrane |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003924 | molecular_function | GTPase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005525 | molecular_function | GTP binding |
| B | 0005681 | cellular_component | spliceosomal complex |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005764 | cellular_component | lysosome |
| B | 0005765 | cellular_component | lysosomal membrane |
| B | 0005783 | cellular_component | endoplasmic reticulum |
| B | 0005789 | cellular_component | endoplasmic reticulum membrane |
| B | 0005794 | cellular_component | Golgi apparatus |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0007165 | biological_process | signal transduction |
| B | 0007264 | biological_process | small GTPase-mediated signal transduction |
| B | 0012505 | cellular_component | endomembrane system |
| B | 0014069 | cellular_component | postsynaptic density |
| B | 0016020 | cellular_component | membrane |
| B | 0016241 | biological_process | regulation of macroautophagy |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0019003 | molecular_function | GDP binding |
| B | 0019901 | molecular_function | protein kinase binding |
| B | 0030295 | molecular_function | protein kinase activator activity |
| B | 0031669 | biological_process | cellular response to nutrient levels |
| B | 0032006 | biological_process | regulation of TOR signaling |
| B | 0032008 | biological_process | positive regulation of TOR signaling |
| B | 0043539 | molecular_function | protein serine/threonine kinase activator activity |
| B | 0045202 | cellular_component | synapse |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0048709 | biological_process | oligodendrocyte differentiation |
| B | 0048714 | biological_process | positive regulation of oligodendrocyte differentiation |
| B | 0051726 | biological_process | regulation of cell cycle |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0120163 | biological_process | negative regulation of cold-induced thermogenesis |
| B | 1904263 | biological_process | positive regulation of TORC1 signaling |
| B | 2000074 | biological_process | regulation of type B pancreatic cell development |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 202 |
| Chain | Residue |
| A | SER20 |
| A | HOH170 |
| A | HOH172 |
| A | HOH176 |
| A | HOH197 |
| A | GDP201 |
| site_id | AC2 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE GDP A 201 |
| Chain | Residue |
| A | LYS19 |
| A | SER20 |
| A | SER21 |
| A | PHE31 |
| A | VAL32 |
| A | ASP33 |
| A | ASN119 |
| A | LYS120 |
| A | ASP122 |
| A | LEU123 |
| A | SER149 |
| A | ALA150 |
| A | HOH172 |
| A | HOH189 |
| A | HOH197 |
| A | MG202 |
| A | HOH235 |
| A | HOH269 |
| A | HOH273 |
| A | HOH290 |
| A | SER16 |
| A | VAL17 |
| A | GLY18 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 178 |
| Chain | Residue |
| B | SER20 |
| B | THR38 |
| B | HOH174 |
| B | GNP179 |
| B | HOH262 |
| site_id | AC4 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE GNP B 179 |
| Chain | Residue |
| B | ARG15 |
| B | SER16 |
| B | VAL17 |
| B | GLY18 |
| B | LYS19 |
| B | SER20 |
| B | SER21 |
| B | PHE31 |
| B | VAL32 |
| B | ASP33 |
| B | ALA35 |
| B | PRO37 |
| B | THR38 |
| B | GLY63 |
| B | ASN119 |
| B | LYS120 |
| B | ASP122 |
| B | LEU123 |
| B | SER149 |
| B | ALA150 |
| B | HOH174 |
| B | MG178 |
| B | HOH215 |
| B | HOH262 |
| B | HOH289 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT B 1 |
| Chain | Residue |
| B | ALA35 |
| B | ASP36 |
| B | THR38 |
| B | ASN41 |
| B | HOH267 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:29236692, ECO:0007744|PDB:1XTS, ECO:0007744|PDB:6BCU |
| Chain | Residue | Details |
| A | SER16 | |
| B | ASN119 | |
| B | ASP122 | |
| B | ALA150 | |
| A | GLY18 | |
| A | ALA35 | |
| A | ASN119 | |
| A | ASP122 | |
| A | ALA150 | |
| B | SER16 | |
| B | GLY18 | |
| B | ALA35 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:22002721, ECO:0000269|PubMed:22819219, ECO:0000269|PubMed:32470140, ECO:0007744|PDB:1XTQ, ECO:0007744|PDB:3SEA, ECO:0007744|PDB:3T5G, ECO:0007744|PDB:7BTA, ECO:0007744|PDB:7BTC |
| Chain | Residue | Details |
| A | VAL17 | |
| B | VAL17 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:22002721, ECO:0000269|PubMed:29236692, ECO:0007744|PDB:1XTS, ECO:0007744|PDB:6BCU |
| Chain | Residue | Details |
| A | LYS19 | |
| B | LYS19 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:29236692, ECO:0000269|PubMed:29416044, ECO:0007744|PDB:5YXH, ECO:0007744|PDB:6BCU, ECO:0007744|PDB:6BSX, ECO:0007744|PDB:6BT0 |
| Chain | Residue | Details |
| A | SER20 | |
| B | SER20 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0007744|PDB:1XTS |
| Chain | Residue | Details |
| A | SER21 | |
| A | VAL32 | |
| B | SER21 | |
| B | VAL32 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:22002721, ECO:0000269|PubMed:22819219, ECO:0000269|PubMed:29416044, ECO:0000269|PubMed:32470140, ECO:0007744|PDB:3SEA, ECO:0007744|PDB:6BT0, ECO:0007744|PDB:7BTA, ECO:0007744|PDB:7BTC |
| Chain | Residue | Details |
| A | ASP33 | |
| B | ASP33 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:29236692, ECO:0007744|PDB:6BCU |
| Chain | Residue | Details |
| A | THR38 | |
| B | THR38 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | SITE: Important for autoinhibition of GTPase activity => ECO:0000269|PubMed:22819219 |
| Chain | Residue | Details |
| A | ALA35 | |
| B | ALA35 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by MAPKAPK5 => ECO:0000250|UniProtKB:Q921J2 |
| Chain | Residue | Details |
| A | SER130 | |
| B | SER130 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:30514904 |
| Chain | Residue | Details |
| A | LYS8 | |
| B | LYS8 |
