3RYO

Crystal Structure of Enhanced Intracellular Survival (Eis) Protein from Mycobacterium tuberculosis with Acetyl CoA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0005829	cellular_component	cytosol
A	0008080	molecular_function	N-acetyltransferase activity
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0030649	biological_process	aminoglycoside antibiotic catabolic process
A	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
A	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
A	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
A	0042802	molecular_function	identical protein binding
A	0043655	cellular_component	host extracellular space
A	0044161	cellular_component	host cell cytoplasmic vesicle
A	0046677	biological_process	response to antibiotic
A	0051701	biological_process	biological process involved in interaction with host
A	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
A	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
A	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
A	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
A	0097691	cellular_component	bacterial extracellular vesicle
B	0005576	cellular_component	extracellular region
B	0005829	cellular_component	cytosol
B	0008080	molecular_function	N-acetyltransferase activity
B	0016746	molecular_function	acyltransferase activity
B	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
B	0030649	biological_process	aminoglycoside antibiotic catabolic process
B	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
B	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
B	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
B	0042802	molecular_function	identical protein binding
B	0043655	cellular_component	host extracellular space
B	0044161	cellular_component	host cell cytoplasmic vesicle
B	0046677	biological_process	response to antibiotic
B	0051701	biological_process	biological process involved in interaction with host
B	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
B	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
B	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
B	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
B	0097691	cellular_component	bacterial extracellular vesicle
C	0005576	cellular_component	extracellular region
C	0005829	cellular_component	cytosol
C	0008080	molecular_function	N-acetyltransferase activity
C	0016746	molecular_function	acyltransferase activity
C	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
C	0030649	biological_process	aminoglycoside antibiotic catabolic process
C	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
C	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
C	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
C	0042802	molecular_function	identical protein binding
C	0043655	cellular_component	host extracellular space
C	0044161	cellular_component	host cell cytoplasmic vesicle
C	0046677	biological_process	response to antibiotic
C	0051701	biological_process	biological process involved in interaction with host
C	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
C	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
C	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
C	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
C	0097691	cellular_component	bacterial extracellular vesicle
D	0005576	cellular_component	extracellular region
D	0005829	cellular_component	cytosol
D	0008080	molecular_function	N-acetyltransferase activity
D	0016746	molecular_function	acyltransferase activity
D	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
D	0030649	biological_process	aminoglycoside antibiotic catabolic process
D	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
D	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
D	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
D	0042802	molecular_function	identical protein binding
D	0043655	cellular_component	host extracellular space
D	0044161	cellular_component	host cell cytoplasmic vesicle
D	0046677	biological_process	response to antibiotic
D	0051701	biological_process	biological process involved in interaction with host
D	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
D	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
D	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
D	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
D	0097691	cellular_component	bacterial extracellular vesicle
E	0005576	cellular_component	extracellular region
E	0005829	cellular_component	cytosol
E	0008080	molecular_function	N-acetyltransferase activity
E	0016746	molecular_function	acyltransferase activity
E	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
E	0030649	biological_process	aminoglycoside antibiotic catabolic process
E	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
E	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
E	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
E	0042802	molecular_function	identical protein binding
E	0043655	cellular_component	host extracellular space
E	0044161	cellular_component	host cell cytoplasmic vesicle
E	0046677	biological_process	response to antibiotic
E	0051701	biological_process	biological process involved in interaction with host
E	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
E	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
E	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
E	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
E	0097691	cellular_component	bacterial extracellular vesicle
F	0005576	cellular_component	extracellular region
F	0005829	cellular_component	cytosol
F	0008080	molecular_function	N-acetyltransferase activity
F	0016746	molecular_function	acyltransferase activity
F	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
F	0030649	biological_process	aminoglycoside antibiotic catabolic process
F	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
F	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
F	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
F	0042802	molecular_function	identical protein binding
F	0043655	cellular_component	host extracellular space
F	0044161	cellular_component	host cell cytoplasmic vesicle
F	0046677	biological_process	response to antibiotic
F	0051701	biological_process	biological process involved in interaction with host
F	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
F	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
F	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
F	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
F	0097691	cellular_component	bacterial extracellular vesicle
G	0005576	cellular_component	extracellular region
G	0005829	cellular_component	cytosol
G	0008080	molecular_function	N-acetyltransferase activity
G	0016746	molecular_function	acyltransferase activity
G	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
G	0030649	biological_process	aminoglycoside antibiotic catabolic process
G	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
G	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
G	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
G	0042802	molecular_function	identical protein binding
G	0043655	cellular_component	host extracellular space
G	0044161	cellular_component	host cell cytoplasmic vesicle
G	0046677	biological_process	response to antibiotic
G	0051701	biological_process	biological process involved in interaction with host
G	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
G	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
G	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
G	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
G	0097691	cellular_component	bacterial extracellular vesicle
H	0005576	cellular_component	extracellular region
H	0005829	cellular_component	cytosol
H	0008080	molecular_function	N-acetyltransferase activity
H	0016746	molecular_function	acyltransferase activity
H	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
H	0030649	biological_process	aminoglycoside antibiotic catabolic process
H	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
H	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
H	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
H	0042802	molecular_function	identical protein binding
H	0043655	cellular_component	host extracellular space
H	0044161	cellular_component	host cell cytoplasmic vesicle
H	0046677	biological_process	response to antibiotic
H	0051701	biological_process	biological process involved in interaction with host
H	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
H	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
H	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
H	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
H	0097691	cellular_component	bacterial extracellular vesicle
I	0005576	cellular_component	extracellular region
I	0005829	cellular_component	cytosol
I	0008080	molecular_function	N-acetyltransferase activity
I	0016746	molecular_function	acyltransferase activity
I	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
I	0030649	biological_process	aminoglycoside antibiotic catabolic process
I	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
I	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
I	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
I	0042802	molecular_function	identical protein binding
I	0043655	cellular_component	host extracellular space
I	0044161	cellular_component	host cell cytoplasmic vesicle
I	0046677	biological_process	response to antibiotic
I	0051701	biological_process	biological process involved in interaction with host
I	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
I	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
I	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
I	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
I	0097691	cellular_component	bacterial extracellular vesicle
J	0005576	cellular_component	extracellular region
J	0005829	cellular_component	cytosol
J	0008080	molecular_function	N-acetyltransferase activity
J	0016746	molecular_function	acyltransferase activity
J	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
J	0030649	biological_process	aminoglycoside antibiotic catabolic process
J	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
J	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
J	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
J	0042802	molecular_function	identical protein binding
J	0043655	cellular_component	host extracellular space
J	0044161	cellular_component	host cell cytoplasmic vesicle
J	0046677	biological_process	response to antibiotic
J	0051701	biological_process	biological process involved in interaction with host
J	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
J	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
J	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
J	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
J	0097691	cellular_component	bacterial extracellular vesicle
K	0005576	cellular_component	extracellular region
K	0005829	cellular_component	cytosol
K	0008080	molecular_function	N-acetyltransferase activity
K	0016746	molecular_function	acyltransferase activity
K	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
K	0030649	biological_process	aminoglycoside antibiotic catabolic process
K	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
K	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
K	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
K	0042802	molecular_function	identical protein binding
K	0043655	cellular_component	host extracellular space
K	0044161	cellular_component	host cell cytoplasmic vesicle
K	0046677	biological_process	response to antibiotic
K	0051701	biological_process	biological process involved in interaction with host
K	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
K	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
K	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
K	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
K	0097691	cellular_component	bacterial extracellular vesicle
L	0005576	cellular_component	extracellular region
L	0005829	cellular_component	cytosol
L	0008080	molecular_function	N-acetyltransferase activity
L	0016746	molecular_function	acyltransferase activity
L	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
L	0030649	biological_process	aminoglycoside antibiotic catabolic process
L	0033661	biological_process	effector-mediated defense to host-produced reactive oxygen species
L	0034054	biological_process	symbiont-mediated suppression of host defense-related programmed cell death
L	0034069	molecular_function	aminoglycoside N-acetyltransferase activity
L	0042802	molecular_function	identical protein binding
L	0043655	cellular_component	host extracellular space
L	0044161	cellular_component	host cell cytoplasmic vesicle
L	0046677	biological_process	response to antibiotic
L	0051701	biological_process	biological process involved in interaction with host
L	0052032	biological_process	symbiont-mediated perturbation of host inflammatory response
L	0052040	biological_process	symbiont-mediated perturbation of host programmed cell death
L	0052167	biological_process	symbiont-mediated perturbation of host innate immune response
L	0061733	molecular_function	peptide-lysine-N-acetyltransferase activity
L	0097691	cellular_component	bacterial extracellular vesicle

Functional Information from PDB Data

site_id	AC1
Number of Residues	24
Details	BINDING SITE FOR RESIDUE ACO A 501

Chain	Residue
A	PHE30
A	LEU102
A	LEU103
A	ARG104
A	HIS125
A	SER127
A	GLU128
A	GLY130
A	ILE131
A	TYR132
A	ARG134
A	SER89
A	HOH622
A	HOH631
A	HOH641
F	ASP266
F	HOH605
A	PHE90
A	VAL91
A	ALA92
A	VAL93
A	ARG98
A	ARG99
A	GLY101

---

site_id	AC2
Number of Residues	22
Details	BINDING SITE FOR RESIDUE ACO B 501

Chain	Residue
B	PHE30
B	SER89
B	PHE90
B	VAL91
B	ALA92
B	VAL93
B	ARG98
B	ARG99
B	ARG100
B	GLY101
B	LEU102
B	LEU103
B	ARG104
B	HIS125
B	SER127
B	GLU128
B	GLY130
B	ILE131
B	TYR132
B	ARG134
B	HOH609
D	ASP266

---

site_id	AC3
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ACO C 501

Chain	Residue
A	ASP266
C	PHE90
C	VAL91
C	ALA92
C	VAL93
C	ARG98
C	ARG99
C	GLY101
C	LEU102
C	LEU103
C	ARG104
C	HIS125
C	SER127
C	GLU128
C	GLY130
C	ILE131
C	TYR132
C	ARG134
C	HOH630
C	HOH646

---

site_id	AC4
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ACO D 501

Chain	Residue
D	PHE90
D	VAL91
D	ALA92
D	VAL93
D	ARG98
D	ARG99
D	ARG100
D	GLY101
D	LEU102
D	LEU103
D	ARG104
D	HIS125
D	SER127
D	GLU128
D	GLY130
D	ILE131
D	TYR132
D	ARG134
E	ASP266

---

site_id	AC5
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ACO E 501

Chain	Residue
E	GLU128
E	GLY130
E	ILE131
E	TYR132
E	ARG134
E	HOH603
B	ASP266
E	SER29
E	PHE90
E	VAL91
E	ALA92
E	VAL93
E	ARG98
E	ARG99
E	GLY101
E	LEU102
E	LEU103
E	ARG104
E	HIS125
E	SER127

---

site_id	AC6
Number of Residues	21
Details	BINDING SITE FOR RESIDUE ACO F 501

Chain	Residue
C	ASP266
F	PHE30
F	SER89
F	PHE90
F	VAL91
F	ALA92
F	VAL93
F	ARG98
F	ARG99
F	GLY101
F	LEU102
F	LEU103
F	ARG104
F	HIS125
F	SER127
F	GLU128
F	GLY130
F	ILE131
F	ARG134
F	HOH620
F	HOH645

---

site_id	AC7
Number of Residues	22
Details	BINDING SITE FOR RESIDUE ACO G 501

Chain	Residue
G	PHE30
G	SER89
G	PHE90
G	VAL91
G	ALA92
G	VAL93
G	ARG98
G	ARG99
G	ARG100
G	GLY101
G	LEU102
G	LEU103
G	ARG104
G	HIS125
G	SER127
G	GLU128
G	GLY130
G	ILE131
G	TYR132
G	ARG134
G	HOH602
L	ASP266

---

site_id	AC8
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ACO H 501

Chain	Residue
H	SER89
H	PHE90
H	VAL91
H	VAL93
H	ARG98
H	ARG99
H	GLY101
H	LEU102
H	LEU103
H	ARG104
H	HIS125
H	SER127
H	GLU128
H	GLY130
H	ILE131
H	TYR132
H	ARG134
H	HOH601
H	HOH623
J	ASP266

---

site_id	AC9
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ACO I 501

Chain	Residue
G	ASP266
I	SER89
I	PHE90
I	VAL91
I	VAL93
I	ARG98
I	ARG99
I	GLY101
I	LEU102
I	LEU103
I	ARG104
I	HIS125
I	SER127
I	GLU128
I	ILE131
I	TYR132
I	ARG134

---

site_id	BC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ACO J 501

Chain	Residue
J	PHE90
J	VAL91
J	VAL93
J	ARG98
J	ARG99
J	ARG100
J	GLY101
J	LEU102
J	LEU103
J	ARG104
J	HIS125
J	SER127
J	GLU128
J	GLY130
J	ILE131
J	TYR132
J	ARG134
K	ASP266

---

site_id	BC2
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ACO K 501

Chain	Residue
H	ASP266
K	PHE90
K	VAL91
K	ALA92
K	VAL93
K	ARG98
K	ARG99
K	ARG100
K	GLY101
K	LEU102
K	LEU103
K	ARG104
K	HIS125
K	SER127
K	GLU128
K	GLY130
K	ILE131
K	TYR132
K	ARG134
K	HOH622

---

site_id	BC3
Number of Residues	21
Details	BINDING SITE FOR RESIDUE ACO L 501

Chain	Residue
I	ASP266
L	PHE30
L	SER89
L	PHE90
L	VAL91
L	ALA92
L	VAL93
L	ARG98
L	ARG99
L	GLY101
L	LEU102
L	LEU103
L	ARG104
L	HIS125
L	SER127
L	GLU128
L	GLY130
L	ILE131
L	TYR132
L	ARG134
L	HOH628

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:21628583

Chain	Residue	Details
A	ALA126
J	ALA126
K	ALA126
L	ALA126
B	ALA126
C	ALA126
D	ALA126
E	ALA126
F	ALA126
G	ALA126
H	ALA126
I	ALA126

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	ACT_SITE: Proton acceptor; via carboxylate => ECO:0000305|PubMed:21628583

Chain	Residue	Details
A	VAL402
J	VAL402
K	VAL402
L	VAL402
B	VAL402
C	VAL402
D	VAL402
E	VAL402
F	VAL402
G	VAL402
H	VAL402
I	VAL402

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269|PubMed:22547814, ECO:0000305|PubMed:21628583, ECO:0007744|PDB:3RYO

Chain	Residue	Details
A	THR85
E	VAL121
F	THR85
F	VAL121
G	THR85
G	VAL121
H	THR85
H	VAL121
I	THR85
I	VAL121
J	THR85
A	VAL121
J	VAL121
K	THR85
K	VAL121
L	THR85
L	VAL121
B	THR85
B	VAL121
C	THR85
C	VAL121
D	THR85
D	VAL121
E	THR85

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:22547814, ECO:0000305|PubMed:21628583, ECO:0000305|PubMed:24106131, ECO:0000305|PubMed:27010218, ECO:0007744|PDB:3RYO

Chain	Residue	Details
A	VAL93
J	VAL93
K	VAL93
L	VAL93
B	VAL93
C	VAL93
D	VAL93
E	VAL93
F	VAL93
G	VAL93
H	VAL93
I	VAL93

---