3RYO
Crystal Structure of Enhanced Intracellular Survival (Eis) Protein from Mycobacterium tuberculosis with Acetyl CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 108.129, 150.190, 184.383 |
Unit cell angles | 90.00, 103.05, 90.00 |
Refinement procedure
Resolution | 29.937 - 2.800 |
R-factor | 0.1946 |
Rwork | 0.192 |
R-free | 0.24770 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.230 |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 269936 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 12%(w/v) PEG 3350, 300mM ammonium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |