Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0009257 | biological_process | 10-formyltetrahydrofolate biosynthetic process |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0031427 | biological_process | response to methotrexate |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0046656 | biological_process | folic acid biosynthetic process |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0050661 | molecular_function | NADP binding |
| A | 0051870 | molecular_function | methotrexate binding |
| A | 0051871 | molecular_function | dihydrofolic acid binding |
| A | 0070401 | molecular_function | NADP+ binding |
| A | 0070402 | molecular_function | NADPH binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE 6ME A 161 |
| Chain | Residue |
| A | ILE5 |
| A | ALA6 |
| A | ASP27 |
| A | PHE31 |
| A | ILE94 |
| A | TYR100 |
| A | THR113 |
| A | HOH214 |
| site_id | AC2 |
| Number of Residues | 34 |
| Details | BINDING SITE FOR RESIDUE NDP A 1160 |
| Chain | Residue |
| A | ALA7 |
| A | ILE14 |
| A | GLY15 |
| A | ASN18 |
| A | ALA19 |
| A | MET20 |
| A | TRP22 |
| A | GLY43 |
| A | ARG44 |
| A | HIS45 |
| A | THR46 |
| A | LEU62 |
| A | SER63 |
| A | SER64 |
| A | LYS76 |
| A | ILE94 |
| A | GLY96 |
| A | GLY97 |
| A | ARG98 |
| A | VAL99 |
| A | TYR100 |
| A | GLN102 |
| A | THR123 |
| A | ASP131 |
| A | CA163 |
| A | CA165 |
| A | HOH212 |
| A | HOH228 |
| A | HOH241 |
| A | HOH252 |
| A | HOH257 |
| A | HOH286 |
| A | HOH311 |
| A | ALA6 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA A 160 |
| Chain | Residue |
| A | VAL10 |
| A | ASP116 |
| A | HOH307 |
| A | HOH356 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE CL A 162 |
| Chain | Residue |
| A | ASP116 |
| A | HIS149 |
| A | SER150 |
| A | CA168 |
| A | CL177 |
| A | HOH181 |
| A | HOH204 |
| A | HOH247 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA A 163 |
| Chain | Residue |
| A | ARG44 |
| A | GLN65 |
| A | NDP1160 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA A 164 |
| Chain | Residue |
| A | ARG52 |
| A | PRO53 |
| A | GLU80 |
| A | HOH305 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA A 165 |
| Chain | Residue |
| A | MET20 |
| A | SER49 |
| A | NDP1160 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA A 166 |
| Chain | Residue |
| A | GLU134 |
| A | GLU157 |
| A | HOH247 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA A 167 |
| Chain | Residue |
| A | PRO105 |
| A | LYS106 |
| A | HOH278 |
| A | HOH326 |
| site_id | BC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CA A 168 |
| Chain | Residue |
| A | ASP116 |
| A | HIS149 |
| A | ARG159 |
| A | CL162 |
| A | CL177 |
| A | HOH247 |
| A | HOH258 |
| site_id | BC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA A 169 |
| Chain | Residue |
| A | ILE82 |
| A | GLY86 |
| A | HOH226 |
| site_id | BC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 170 |
| Chain | Residue |
| A | GLU101 |
| A | ARG159 |
| A | CL177 |
| A | HOH225 |
| A | HOH258 |
| site_id | BC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CA A 171 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 172 |
| Chain | Residue |
| A | GLY121 |
| A | HIS124 |
| A | PHE140 |
| A | CYS152 |
| A | HOH249 |
| site_id | BC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CA A 173 |
| Chain | Residue |
| A | ASP11 |
| A | HOH253 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA A 174 |
| Chain | Residue |
| A | VAL78 |
| A | ASP79 |
| A | ASP131 |
| A | HOH327 |
| site_id | BC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA A 175 |
| Chain | Residue |
| A | ARG12 |
| A | ASP127 |
| A | TYR128 |
| site_id | BC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL A 176 |
| Chain | Residue |
| A | LYS32 |
| A | PRO55 |
| A | ARG57 |
| site_id | CC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE CL A 177 |
| Chain | Residue |
| A | GLU101 |
| A | ASP116 |
| A | ARG159 |
| A | CL162 |
| A | CA168 |
| A | CA170 |
| A | HOH258 |
| A | HOH307 |
| site_id | CC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 178 |
| Chain | Residue |
| A | ASP116 |
| A | ASP122 |
| A | HOH259 |
| A | HOH301 |
| A | HOH318 |
| site_id | CC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CA A 179 |
| site_id | CC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA A 180 |
| Chain | Residue |
| A | ARG52 |
| A | HOH296 |
| A | HOH346 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DlawFkrnT |
| Chain | Residue | Details |
| A | VAL13-THR35 | |
Functional Information from SwissProt/UniProt
| Chain | Residue | Details |
| A | ILE5 | |
| A | ASP27 | |
| A | ARG52 | |
| A | ARG57 | |
| A | THR113 | |
| Chain | Residue | Details |
| A | ALA7 | |
| A | VAL13 | |
| A | HIS45 | |
| A | SER63 | |
| A | LYS76 | |
| A | GLY95 | |
