3PYM
Structure of GAPDH 3 from S.cerevisiae at 2.0 A resolution
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0003723 | molecular_function | RNA binding |
A | 0003729 | molecular_function | mRNA binding |
A | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
A | 0005634 | cellular_component | nucleus |
A | 0005737 | cellular_component | cytoplasm |
A | 0005739 | cellular_component | mitochondrion |
A | 0005811 | cellular_component | lipid droplet |
A | 0005829 | cellular_component | cytosol |
A | 0005886 | cellular_component | plasma membrane |
A | 0006006 | biological_process | glucose metabolic process |
A | 0006094 | biological_process | gluconeogenesis |
A | 0006096 | biological_process | glycolytic process |
A | 0006915 | biological_process | apoptotic process |
A | 0009277 | cellular_component | fungal-type cell wall |
A | 0015886 | biological_process | heme transport |
A | 0016491 | molecular_function | oxidoreductase activity |
A | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
A | 0020037 | molecular_function | heme binding |
A | 0030312 | cellular_component | external encapsulating structure |
A | 0050661 | molecular_function | NADP binding |
A | 0051287 | molecular_function | NAD binding |
A | 0072593 | biological_process | reactive oxygen species metabolic process |
A | 1904408 | molecular_function | melatonin binding |
B | 0003723 | molecular_function | RNA binding |
B | 0003729 | molecular_function | mRNA binding |
B | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
B | 0005634 | cellular_component | nucleus |
B | 0005737 | cellular_component | cytoplasm |
B | 0005739 | cellular_component | mitochondrion |
B | 0005811 | cellular_component | lipid droplet |
B | 0005829 | cellular_component | cytosol |
B | 0005886 | cellular_component | plasma membrane |
B | 0006006 | biological_process | glucose metabolic process |
B | 0006094 | biological_process | gluconeogenesis |
B | 0006096 | biological_process | glycolytic process |
B | 0006915 | biological_process | apoptotic process |
B | 0009277 | cellular_component | fungal-type cell wall |
B | 0015886 | biological_process | heme transport |
B | 0016491 | molecular_function | oxidoreductase activity |
B | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
B | 0020037 | molecular_function | heme binding |
B | 0030312 | cellular_component | external encapsulating structure |
B | 0050661 | molecular_function | NADP binding |
B | 0051287 | molecular_function | NAD binding |
B | 0072593 | biological_process | reactive oxygen species metabolic process |
B | 1904408 | molecular_function | melatonin binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 24 |
Details | BINDING SITE FOR RESIDUE NAD A 333 |
Chain | Residue |
A | ASN7 |
A | THR97 |
A | GLY98 |
A | THR120 |
A | ALA121 |
A | CYS150 |
A | ALA181 |
A | ASN314 |
A | TYR318 |
A | HOH360 |
A | HOH377 |
A | GLY8 |
A | HOH409 |
A | HOH447 |
A | HOH566 |
B | HOH380 |
B | HOH456 |
A | GLY10 |
A | ARG11 |
A | ILE12 |
A | ASP33 |
A | PRO34 |
A | ARG78 |
A | SER96 |
site_id | AC2 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE MRY A 334 |
Chain | Residue |
A | ALA42 |
A | TYR43 |
A | VAL58 |
A | HIS60 |
A | HOH385 |
B | ASP277 |
site_id | AC3 |
Number of Residues | 20 |
Details | BINDING SITE FOR RESIDUE NAD B 333 |
Chain | Residue |
A | HOH397 |
B | ASN7 |
B | GLY8 |
B | GLY10 |
B | ARG11 |
B | ILE12 |
B | ASP33 |
B | PRO34 |
B | SER96 |
B | THR97 |
B | GLY98 |
B | THR120 |
B | ALA121 |
B | CYS150 |
B | ALA181 |
B | ASN314 |
B | TYR318 |
B | HOH400 |
B | HOH411 |
B | HOH534 |
site_id | AC4 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE MRY B 334 |
Chain | Residue |
A | ASP277 |
B | ALA42 |
B | TYR43 |
B | VAL58 |
B | HOH379 |
B | HOH434 |
site_id | AC5 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE NA B 504 |
Chain | Residue |
B | ALA20 |
B | LEU21 |
B | ARG23 |
B | VAL26 |
B | HOH519 |
Functional Information from PROSITE/UniProt
site_id | PS00071 |
Number of Residues | 8 |
Details | GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL |
Chain | Residue | Details |
A | ALA148-LEU155 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 2 |
Details | ACT_SITE: Nucleophile => ECO:0000255|PROSITE-ProRule:PRU10009 |
Chain | Residue | Details |
A | CYS150 | |
B | CYS150 |
site_id | SWS_FT_FI2 |
Number of Residues | 12 |
Details | BINDING: BINDING => ECO:0007744|PDB:3PYM |
Chain | Residue | Details |
A | ARG11 | |
B | THR120 | |
B | ASN314 | |
B | TYR318 | |
A | ILE12 | |
A | ASP33 | |
A | THR120 | |
A | ASN314 | |
A | TYR318 | |
B | ARG11 | |
B | ILE12 | |
B | ASP33 |
site_id | SWS_FT_FI3 |
Number of Residues | 8 |
Details | BINDING: BINDING => ECO:0000250|UniProtKB:P22513 |
Chain | Residue | Details |
A | SER149 | |
A | THR180 | |
A | THR209 | |
A | ARG232 | |
B | SER149 | |
B | THR180 | |
B | THR209 | |
B | ARG232 |
site_id | SWS_FT_FI4 |
Number of Residues | 2 |
Details | SITE: Activates thiol group during catalysis => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
A | HIS177 | |
B | HIS177 |
site_id | SWS_FT_FI5 |
Number of Residues | 2 |
Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956 |
Chain | Residue | Details |
A | SER302 | |
B | SER302 |
site_id | SWS_FT_FI6 |
Number of Residues | 6 |
Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
Chain | Residue | Details |
A | LYS46 | |
A | LYS63 | |
B | LYS46 | |
B | LYS63 |