3PYM
Structure of GAPDH 3 from S.cerevisiae at 2.0 A resolution
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Detector technology | CCD |
| Collection date | 2004-01-04 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9340 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 121.834, 131.052, 96.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.615 - 2.000 |
| R-factor | 0.1516 |
| Rwork | 0.147 |
| R-free | 0.18880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gd1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER ((CCP4 supported)) |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.340 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.094 |
| Number of reflections | 52575 |
| Completeness [%] | 99.9 |
| Redundancy | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 10.5-15.5% PEG, 50mM NaAc, 1mm DTT, 1mm NAD, pH 7.0, VAPOR DIFFUSION, temperature 293K |
