3PIA

Site-specific Glycosylation of Hemoglobin Utilizing Oxime Ligation Chemistry as a Viable Alternative to PEGylation

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004601	molecular_function	peroxidase activity
A	0005344	molecular_function	oxygen carrier activity
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005833	cellular_component	hemoglobin complex
A	0015671	biological_process	oxygen transport
A	0019825	molecular_function	oxygen binding
A	0020037	molecular_function	heme binding
A	0031720	molecular_function	haptoglobin binding
A	0031838	cellular_component	haptoglobin-hemoglobin complex
A	0042744	biological_process	hydrogen peroxide catabolic process
A	0043177	molecular_function	organic acid binding
A	0046872	molecular_function	metal ion binding
A	0098869	biological_process	cellular oxidant detoxification
B	0004601	molecular_function	peroxidase activity
B	0005344	molecular_function	oxygen carrier activity
B	0005833	cellular_component	hemoglobin complex
B	0015671	biological_process	oxygen transport
B	0019825	molecular_function	oxygen binding
B	0020037	molecular_function	heme binding
B	0031720	molecular_function	haptoglobin binding
B	0031721	molecular_function	hemoglobin alpha binding
B	0031838	cellular_component	haptoglobin-hemoglobin complex
B	0042744	biological_process	hydrogen peroxide catabolic process
B	0043177	molecular_function	organic acid binding
B	0046872	molecular_function	metal ion binding
B	0098869	biological_process	cellular oxidant detoxification
C	0004601	molecular_function	peroxidase activity
C	0005344	molecular_function	oxygen carrier activity
C	0005506	molecular_function	iron ion binding
C	0005515	molecular_function	protein binding
C	0005833	cellular_component	hemoglobin complex
C	0015671	biological_process	oxygen transport
C	0019825	molecular_function	oxygen binding
C	0020037	molecular_function	heme binding
C	0031720	molecular_function	haptoglobin binding
C	0031838	cellular_component	haptoglobin-hemoglobin complex
C	0042744	biological_process	hydrogen peroxide catabolic process
C	0043177	molecular_function	organic acid binding
C	0046872	molecular_function	metal ion binding
C	0098869	biological_process	cellular oxidant detoxification
D	0004601	molecular_function	peroxidase activity
D	0005344	molecular_function	oxygen carrier activity
D	0005833	cellular_component	hemoglobin complex
D	0015671	biological_process	oxygen transport
D	0019825	molecular_function	oxygen binding
D	0020037	molecular_function	heme binding
D	0031720	molecular_function	haptoglobin binding
D	0031721	molecular_function	hemoglobin alpha binding
D	0031838	cellular_component	haptoglobin-hemoglobin complex
D	0042744	biological_process	hydrogen peroxide catabolic process
D	0043177	molecular_function	organic acid binding
D	0046872	molecular_function	metal ion binding
D	0098869	biological_process	cellular oxidant detoxification

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE HEM A 142

Chain	Residue
A	TYR42
A	ASN97
A	PHE98
A	LEU101
A	VAL132
A	LEU136
A	CMO143
A	PHE43
A	HIS45
A	HIS58
A	LYS61
A	LEU83
A	LEU86
A	HIS87
A	LEU91

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CMO A 143

Chain	Residue
A	HIS58
A	VAL62
A	HEM142

---

site_id	AC3
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM B 147

Chain	Residue
B	THR38
B	PHE41
B	PHE42
B	HIS63
B	SER70
B	PHE71
B	LEU91
B	HIS92
B	LEU96
B	ASN102
B	LEU106
B	VAL137
B	LEU141
B	CMO148

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CMO B 148

Chain	Residue
B	HIS63
B	VAL67
B	HEM147

---

site_id	AC5
Number of Residues	18
Details	BINDING SITE FOR RESIDUE HEM C 142

Chain	Residue
A	ALA12
A	LYS16
C	TYR42
C	PHE43
C	HIS45
C	HIS58
C	LYS61
C	LEU83
C	LEU86
C	HIS87
C	LEU91
C	VAL93
C	ASN97
C	PHE98
C	LEU101
C	CMO143
C	HOH202
C	HOH210

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CMO C 143

Chain	Residue
C	HIS58
C	VAL62
C	HEM142

---

site_id	AC7
Number of Residues	13
Details	BINDING SITE FOR RESIDUE HEM D 147

Chain	Residue
D	THR38
D	PHE41
D	PHE42
D	PHE45
D	HIS63
D	SER70
D	PHE71
D	HIS92
D	LEU96
D	ASN102
D	PHE103
D	LEU141
D	CMO148

---

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CMO D 148

Chain	Residue
D	HIS63
D	VAL67
D	HEM147

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00238

Chain	Residue	Details
A	HIS58
C	HIS58

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: proximal binding residue => ECO:0000255|PROSITE-ProRule:PRU00238

Chain	Residue	Details
A	HIS87
C	HIS87

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P69905

Chain	Residue	Details
A	SER3
A	SER35
A	SER49
C	SER3
C	SER35
C	SER49

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:P01942

Chain	Residue	Details
A	LYS7
A	LYS11
A	LYS40
C	LYS7
C	LYS11
C	LYS40

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P01942

Chain	Residue	Details
A	LYS16
C	LYS16
D	LYS59
D	LYS82

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P69905

Chain	Residue	Details
A	TYR24
C	TYR24

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P01942

Chain	Residue	Details
A	SER102
A	SER124
A	SER138
C	SER102
C	SER124
C	SER138

---

site_id	SWS_FT_FI8
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P01942

Chain	Residue	Details
A	THR108
A	THR134
A	THR137
C	THR108
C	THR134
C	THR137

---