3PIA
Site-specific Glycosylation of Hemoglobin Utilizing Oxime Ligation Chemistry as a Viable Alternative to PEGylation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2010-10-29 |
Detector | RIGAKU |
Wavelength(s) | 1.5 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.730, 73.210, 128.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.060 - 2.100 |
R-factor | 0.19349 |
Rwork | 0.191 |
R-free | 0.23806 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g0a |
RMSD bond length | 0.021 |
RMSD bond angle | 1.685 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.460 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 35179 | |
Completeness [%] | 99.4 | 95.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under Oil | 9.5 | 293 | PEG MME 2250, pH 9.5, Microbatch under Oil, temperature 293K |