3PB0

Characterisation of the first monomeric dihydrodipicolinate synthase variant reveals evolutionary insights

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
A	0009085	biological_process	lysine biosynthetic process
A	0009089	biological_process	lysine biosynthetic process via diaminopimelate
A	0016829	molecular_function	lyase activity
A	0019877	biological_process	diaminopimelate biosynthetic process
A	0044281	biological_process	small molecule metabolic process
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
B	0009085	biological_process	lysine biosynthetic process
B	0009089	biological_process	lysine biosynthetic process via diaminopimelate
B	0016829	molecular_function	lyase activity
B	0019877	biological_process	diaminopimelate biosynthetic process
B	0044281	biological_process	small molecule metabolic process
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
C	0009085	biological_process	lysine biosynthetic process
C	0009089	biological_process	lysine biosynthetic process via diaminopimelate
C	0016829	molecular_function	lyase activity
C	0019877	biological_process	diaminopimelate biosynthetic process
C	0044281	biological_process	small molecule metabolic process
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0008840	molecular_function	4-hydroxy-tetrahydrodipicolinate synthase activity
D	0009085	biological_process	lysine biosynthetic process
D	0009089	biological_process	lysine biosynthetic process via diaminopimelate
D	0016829	molecular_function	lyase activity
D	0019877	biological_process	diaminopimelate biosynthetic process
D	0044281	biological_process	small molecule metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE SO4 A 295

Chain	Residue
A	ALA166
C	ALA167
C	HOH340
A	ALA167
A	ARG193
A	HOH309
A	HOH353
A	HOH377
A	HOH556
A	HOH791
C	ALA166

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site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 C 295

Chain	Residue
C	THR78
C	ASN79
C	LYS83
C	TYR105
C	TYR106
C	HOH362
C	HOH385
C	HOH703

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 D 295

Chain	Residue
D	ALA166
D	ALA167
D	ARG193
D	HOH756
D	HOH812
D	HOH1001

---

Functional Information from PROSITE/UniProt

site_id	PS00665
Number of Residues	18
Details	DHDPS_1 Dihydrodipicolinate synthase signature 1. ALIvlGTTGESptvnedE

Chain	Residue	Details
A	ALA37-GLU54

---

site_id	PS00666
Number of Residues	32
Details	DHDPS_2 Dihydrodipicolinate synthase signature 2. YNVPgrTgvnVlpetaariaadlknVvGIKEA

Chain	Residue	Details
A	TYR132-ALA163

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton donor/acceptor => ECO:0000255|HAMAP-Rule:MF_00418

Chain	Residue	Details
A	TYR132
B	TYR132
C	TYR132
D	TYR132

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Schiff-base intermediate with substrate => ECO:0000255|HAMAP-Rule:MF_00418

Chain	Residue	Details
A	LYS161
B	LYS161
C	LYS161
D	LYS161

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00418

Chain	Residue	Details
A	THR44
A	ILE206
C	THR44
C	ILE206
D	THR44
D	ILE206
B	THR44
B	ILE206

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	SITE: Part of a proton relay during catalysis => ECO:0000255|HAMAP-Rule:MF_00418

Chain	Residue	Details
C	THR43
C	TYR106
D	THR43
D	TYR106
A	THR43
A	TYR106
B	THR43
B	TYR106

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