Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3OOJ

C1A mutant of E. coli GlmS in complex with glucose-6P and glutamate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005975	biological_process	carbohydrate metabolic process
A	0006002	biological_process	fructose 6-phosphate metabolic process
A	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
A	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
A	0006487	biological_process	protein N-linked glycosylation
A	0006541	biological_process	glutamine metabolic process
A	0008483	molecular_function	transaminase activity
A	0097367	molecular_function	carbohydrate derivative binding
A	1901135	biological_process	carbohydrate derivative metabolic process
A	1901137	biological_process	carbohydrate derivative biosynthetic process
B	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005975	biological_process	carbohydrate metabolic process
B	0006002	biological_process	fructose 6-phosphate metabolic process
B	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
B	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
B	0006487	biological_process	protein N-linked glycosylation
B	0006541	biological_process	glutamine metabolic process
B	0008483	molecular_function	transaminase activity
B	0097367	molecular_function	carbohydrate derivative binding
B	1901135	biological_process	carbohydrate derivative metabolic process
B	1901137	biological_process	carbohydrate derivative biosynthetic process
C	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005975	biological_process	carbohydrate metabolic process
C	0006002	biological_process	fructose 6-phosphate metabolic process
C	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
C	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
C	0006487	biological_process	protein N-linked glycosylation
C	0006541	biological_process	glutamine metabolic process
C	0008483	molecular_function	transaminase activity
C	0097367	molecular_function	carbohydrate derivative binding
C	1901135	biological_process	carbohydrate derivative metabolic process
C	1901137	biological_process	carbohydrate derivative biosynthetic process
D	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005975	biological_process	carbohydrate metabolic process
D	0006002	biological_process	fructose 6-phosphate metabolic process
D	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
D	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
D	0006487	biological_process	protein N-linked glycosylation
D	0006541	biological_process	glutamine metabolic process
D	0008483	molecular_function	transaminase activity
D	0097367	molecular_function	carbohydrate derivative binding
D	1901135	biological_process	carbohydrate derivative metabolic process
D	1901137	biological_process	carbohydrate derivative biosynthetic process
E	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005975	biological_process	carbohydrate metabolic process
E	0006002	biological_process	fructose 6-phosphate metabolic process
E	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
E	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
E	0006487	biological_process	protein N-linked glycosylation
E	0006541	biological_process	glutamine metabolic process
E	0008483	molecular_function	transaminase activity
E	0097367	molecular_function	carbohydrate derivative binding
E	1901135	biological_process	carbohydrate derivative metabolic process
E	1901137	biological_process	carbohydrate derivative biosynthetic process
F	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005975	biological_process	carbohydrate metabolic process
F	0006002	biological_process	fructose 6-phosphate metabolic process
F	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
F	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
F	0006487	biological_process	protein N-linked glycosylation
F	0006541	biological_process	glutamine metabolic process
F	0008483	molecular_function	transaminase activity
F	0097367	molecular_function	carbohydrate derivative binding
F	1901135	biological_process	carbohydrate derivative metabolic process
F	1901137	biological_process	carbohydrate derivative biosynthetic process
G	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005975	biological_process	carbohydrate metabolic process
G	0006002	biological_process	fructose 6-phosphate metabolic process
G	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
G	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
G	0006487	biological_process	protein N-linked glycosylation
G	0006541	biological_process	glutamine metabolic process
G	0008483	molecular_function	transaminase activity
G	0097367	molecular_function	carbohydrate derivative binding
G	1901135	biological_process	carbohydrate derivative metabolic process
G	1901137	biological_process	carbohydrate derivative biosynthetic process
H	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005975	biological_process	carbohydrate metabolic process
H	0006002	biological_process	fructose 6-phosphate metabolic process
H	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
H	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
H	0006487	biological_process	protein N-linked glycosylation
H	0006541	biological_process	glutamine metabolic process
H	0008483	molecular_function	transaminase activity
H	0097367	molecular_function	carbohydrate derivative binding
H	1901135	biological_process	carbohydrate derivative metabolic process
H	1901137	biological_process	carbohydrate derivative biosynthetic process

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Nucleophile; for GATase activity => ECO:0000250

Chain	Residue	Details
A	ALA1
B	ALA1
C	ALA1
D	ALA1
E	ALA1
F	ALA1
G	ALA1
H	ALA1

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: For Fru-6P isomerization activity

Chain	Residue	Details
A	LYS603
B	LYS603
C	LYS603
D	LYS603
E	LYS603
F	LYS603
G	LYS603
H	LYS603

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
A	ALA1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
A	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
A	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
A	TRP74	electrostatic stabiliser, hydrogen bond donor
A	ASN98	electrostatic stabiliser, hydrogen bond donor
A	GLY99	electrostatic stabiliser, hydrogen bond donor
A	GLU481	electrostatic stabiliser, hydrogen bond acceptor
A	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA2
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
B	ALA1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
B	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
B	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
B	TRP74	electrostatic stabiliser, hydrogen bond donor
B	ASN98	electrostatic stabiliser, hydrogen bond donor
B	GLY99	electrostatic stabiliser, hydrogen bond donor
B	GLU481	electrostatic stabiliser, hydrogen bond acceptor
B	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA3
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
C	ALA1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
C	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
C	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
C	TRP74	electrostatic stabiliser, hydrogen bond donor
C	ASN98	electrostatic stabiliser, hydrogen bond donor
C	GLY99	electrostatic stabiliser, hydrogen bond donor
C	GLU481	electrostatic stabiliser, hydrogen bond acceptor
C	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
C	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
C	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA4
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
D	ALA1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
D	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
D	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
D	TRP74	electrostatic stabiliser, hydrogen bond donor
D	ASN98	electrostatic stabiliser, hydrogen bond donor
D	GLY99	electrostatic stabiliser, hydrogen bond donor
D	GLU481	electrostatic stabiliser, hydrogen bond acceptor
D	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
D	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
D	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA5
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
E	ALA1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
E	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
E	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
E	TRP74	electrostatic stabiliser, hydrogen bond donor
E	ASN98	electrostatic stabiliser, hydrogen bond donor
E	GLY99	electrostatic stabiliser, hydrogen bond donor
E	GLU481	electrostatic stabiliser, hydrogen bond acceptor
E	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
E	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
E	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA6
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
F	ALA1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
F	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
F	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
F	TRP74	electrostatic stabiliser, hydrogen bond donor
F	ASN98	electrostatic stabiliser, hydrogen bond donor
F	GLY99	electrostatic stabiliser, hydrogen bond donor
F	GLU481	electrostatic stabiliser, hydrogen bond acceptor
F	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
F	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
F	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA7
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
G	ALA1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
G	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
G	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
G	TRP74	electrostatic stabiliser, hydrogen bond donor
G	ASN98	electrostatic stabiliser, hydrogen bond donor
G	GLY99	electrostatic stabiliser, hydrogen bond donor
G	GLU481	electrostatic stabiliser, hydrogen bond acceptor
G	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
G	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
G	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA8
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
H	ALA1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
H	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
H	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
H	TRP74	electrostatic stabiliser, hydrogen bond donor
H	ASN98	electrostatic stabiliser, hydrogen bond donor
H	GLY99	electrostatic stabiliser, hydrogen bond donor
H	GLU481	electrostatic stabiliser, hydrogen bond acceptor
H	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
H	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
H	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)