3OOJ
C1A mutant of E. coli GlmS in complex with glucose-6P and glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | ADSC |
| Wavelength(s) | 0.933 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 247.599, 247.599, 630.862 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.993 - 2.500 |
| R-factor | 0.1805 |
| Rwork | 0.178 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.178 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.648 | |
| Number of reflections | 250723 | |
| <I/σ(I)> | 5.3 | 1.1 |
| Completeness [%] | 98.6 | 91.3 |
| Redundancy | 7.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 22% PEG3350, 0.2 ammonium acetate, 0.1M HEPES pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
