3O4R

Crystal Structure of Human Dehydrogenase/Reductase (SDR family) member 4 (DHRS4)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000253	molecular_function	3-keto sterol reductase activity
A	0001758	molecular_function	retinal dehydrogenase activity
A	0004090	molecular_function	carbonyl reductase (NADPH) activity
A	0005634	cellular_component	nucleus
A	0005739	cellular_component	mitochondrion
A	0005777	cellular_component	peroxisome
A	0005778	cellular_component	peroxisomal membrane
A	0005782	cellular_component	peroxisomal matrix
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005829	cellular_component	cytosol
A	0006066	biological_process	alcohol metabolic process
A	0008202	biological_process	steroid metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016655	molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
A	0018455	molecular_function	alcohol dehydrogenase [NAD(P)+] activity
A	0033703	molecular_function	3beta-hydroxy-5beta-steroid dehydrogenase activity
A	0042180	biological_process	cellular ketone metabolic process
A	0042572	biological_process	retinol metabolic process
A	0042574	biological_process	retinal metabolic process
A	0042802	molecular_function	identical protein binding
A	0052650	molecular_function	all-trans-retinol dehydrogenase (NADP+) activity
A	2000379	biological_process	positive regulation of reactive oxygen species metabolic process
B	0000253	molecular_function	3-keto sterol reductase activity
B	0001758	molecular_function	retinal dehydrogenase activity
B	0004090	molecular_function	carbonyl reductase (NADPH) activity
B	0005634	cellular_component	nucleus
B	0005739	cellular_component	mitochondrion
B	0005777	cellular_component	peroxisome
B	0005778	cellular_component	peroxisomal membrane
B	0005782	cellular_component	peroxisomal matrix
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005829	cellular_component	cytosol
B	0006066	biological_process	alcohol metabolic process
B	0008202	biological_process	steroid metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016655	molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
B	0018455	molecular_function	alcohol dehydrogenase [NAD(P)+] activity
B	0033703	molecular_function	3beta-hydroxy-5beta-steroid dehydrogenase activity
B	0042180	biological_process	cellular ketone metabolic process
B	0042572	biological_process	retinol metabolic process
B	0042574	biological_process	retinal metabolic process
B	0042802	molecular_function	identical protein binding
B	0052650	molecular_function	all-trans-retinol dehydrogenase (NADP+) activity
B	2000379	biological_process	positive regulation of reactive oxygen species metabolic process
C	0000253	molecular_function	3-keto sterol reductase activity
C	0001758	molecular_function	retinal dehydrogenase activity
C	0004090	molecular_function	carbonyl reductase (NADPH) activity
C	0005634	cellular_component	nucleus
C	0005739	cellular_component	mitochondrion
C	0005777	cellular_component	peroxisome
C	0005778	cellular_component	peroxisomal membrane
C	0005782	cellular_component	peroxisomal matrix
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005829	cellular_component	cytosol
C	0006066	biological_process	alcohol metabolic process
C	0008202	biological_process	steroid metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016655	molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
C	0018455	molecular_function	alcohol dehydrogenase [NAD(P)+] activity
C	0033703	molecular_function	3beta-hydroxy-5beta-steroid dehydrogenase activity
C	0042180	biological_process	cellular ketone metabolic process
C	0042572	biological_process	retinol metabolic process
C	0042574	biological_process	retinal metabolic process
C	0042802	molecular_function	identical protein binding
C	0052650	molecular_function	all-trans-retinol dehydrogenase (NADP+) activity
C	2000379	biological_process	positive regulation of reactive oxygen species metabolic process
D	0000253	molecular_function	3-keto sterol reductase activity
D	0001758	molecular_function	retinal dehydrogenase activity
D	0004090	molecular_function	carbonyl reductase (NADPH) activity
D	0005634	cellular_component	nucleus
D	0005739	cellular_component	mitochondrion
D	0005777	cellular_component	peroxisome
D	0005778	cellular_component	peroxisomal membrane
D	0005782	cellular_component	peroxisomal matrix
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0005829	cellular_component	cytosol
D	0006066	biological_process	alcohol metabolic process
D	0008202	biological_process	steroid metabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0016655	molecular_function	oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
D	0018455	molecular_function	alcohol dehydrogenase [NAD(P)+] activity
D	0033703	molecular_function	3beta-hydroxy-5beta-steroid dehydrogenase activity
D	0042180	biological_process	cellular ketone metabolic process
D	0042572	biological_process	retinol metabolic process
D	0042574	biological_process	retinal metabolic process
D	0042802	molecular_function	identical protein binding
D	0052650	molecular_function	all-trans-retinol dehydrogenase (NADP+) activity
D	2000379	biological_process	positive regulation of reactive oxygen species metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	33
Details	BINDING SITE FOR RESIDUE NAP A 601

Chain	Residue
A	ALA39
A	CYS88
A	HIS89
A	VAL90
A	ASN116
A	ALA118
A	SER168
A	SER169
A	TYR182
A	LYS186
A	PRO212
A	THR41
A	GLY213
A	LEU214
A	ILE215
A	THR217
A	PHE219
A	SER220
A	HOH280
A	HOH299
A	HOH330
A	HOH331
A	ASP42
A	HOH340
A	HOH341
A	HOH346
A	HOH368
A	GLY43
A	ILE44
A	SER63
A	ARG64
A	LYS65
A	ASN68

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 703

Chain	Residue
A	ASN204
A	ARG206
A	HOH305
A	HOH389
C	ARG240

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EPE A 705

Chain	Residue
A	ARG64
A	HIS89
A	HOH341

---

site_id	AC4
Number of Residues	34
Details	BINDING SITE FOR RESIDUE NAP B 602

Chain	Residue
B	ALA39
B	THR41
B	ASP42
B	GLY43
B	ILE44
B	SER63
B	ARG64
B	LYS65
B	ASN68
B	CYS88
B	HIS89
B	VAL90
B	ASN116
B	ALA118
B	SER168
B	SER169
B	TYR182
B	LYS186
B	PRO212
B	GLY213
B	ILE215
B	THR217
B	PHE219
B	SER220
B	HOH280
B	HOH285
B	HOH314
B	HOH316
B	HOH321
B	HOH334
B	HOH383
B	HOH384
B	HOH486
B	GOL707

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO B 702

Chain	Residue
B	PHE123
B	SER125
D	ASP128

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO B 704

Chain	Residue
B	ARG240
B	HOH301
B	HOH407
D	ALA201
D	ASN204
D	ARG206

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL B 707

Chain	Residue
B	SER169
B	TYR182
B	NAP602

---

site_id	AC8
Number of Residues	32
Details	BINDING SITE FOR RESIDUE NAP C 604

Chain	Residue
C	ASN116
C	ALA118
C	SER168
C	SER169
C	TYR182
C	LYS186
C	PRO212
C	GLY213
C	ILE215
C	THR217
C	PHE219
C	SER220
C	HOH279
C	HOH300
C	HOH302
C	HOH308
C	HOH318
C	HOH501
C	HOH607
C	GOL706
C	ALA39
C	THR41
C	ASP42
C	GLY43
C	ILE44
C	SER63
C	ARG64
C	LYS65
C	ASN68
C	CYS88
C	HIS89
C	VAL90

---

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL C 706

Chain	Residue
C	SER169
C	TYR182
C	NAP604

---

site_id	BC1
Number of Residues	32
Details	BINDING SITE FOR RESIDUE NAP D 603

Chain	Residue
D	HOH14
D	ALA39
D	THR41
D	ASP42
D	GLY43
D	ILE44
D	SER63
D	ARG64
D	LYS65
D	ASN68
D	CYS88
D	HIS89
D	VAL90
D	ASN116
D	ALA118
D	SER168
D	SER169
D	TYR182
D	LYS186
D	PRO212
D	GLY213
D	ILE215
D	THR217
D	PHE219
D	SER220
D	HOH291
D	HOH306
D	HOH313
D	HOH315
D	HOH338
D	HOH436
D	HOH438

---

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO D 701

Chain	Residue
B	ASN204
D	ARG240
D	HOH320
D	HOH397

---

Functional Information from PROSITE/UniProt

site_id	PS00061
Number of Residues	29
Details	ADH_SHORT Short-chain dehydrogenases/reductases family signature. SiaafspspgFspYNVSKTALlGLTkTLA

Chain	Residue	Details
A	SER169-ALA197

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU10001

Chain	Residue	Details
A	LEU200
B	LEU200
C	LEU200
D	LEU200

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q8WNV7

Chain	Residue	Details
A	GLN54
A	ASN204
B	GLN54
B	ASN204
C	GLN54
C	ASN204
D	GLN54
D	ASN204

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q99714

Chain	Residue	Details
A	THR187
B	THR187
C	THR187
D	THR187

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	SITE: Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin => ECO:0000269|PubMed:19056333

Chain	Residue	Details
A	LYS194
A	ALA197
B	LYS194
B	ALA197
C	LYS194
C	ALA197
D	LYS194
D	ALA197

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:Q99LB2

Chain	Residue	Details
A	ILE110
A	LYS234
B	ILE110
B	LYS234
C	ILE110
C	LYS234
D	ILE110
D	LYS234

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q99LB2

Chain	Residue	Details
A	PHE123
B	PHE123
C	PHE123
D	PHE123

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q99LB2

Chain	Residue	Details
A	ARG238
B	ARG238
C	ARG238
D	ARG238

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q99LB2

Chain	Residue	Details
A	PRO245
A	VAL252
B	PRO245
B	VAL252
C	PRO245
C	VAL252
D	PRO245
D	VAL252

---