3N23

Crystal structure of the high affinity complex between ouabain and the E2P form of the sodium-potassium pump

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0002028	biological_process	regulation of sodium ion transport
A	0005215	molecular_function	transporter activity
A	0005391	molecular_function	P-type sodium:potassium-exchanging transporter activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005886	cellular_component	plasma membrane
A	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
A	0006813	biological_process	potassium ion transport
A	0006814	biological_process	sodium ion transport
A	0006883	biological_process	intracellular sodium ion homeostasis
A	0008556	molecular_function	P-type potassium transmembrane transporter activity
A	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
A	0016020	cellular_component	membrane
A	0016323	cellular_component	basolateral plasma membrane
A	0016887	molecular_function	ATP hydrolysis activity
A	0030007	biological_process	intracellular potassium ion homeostasis
A	0030424	cellular_component	axon
A	0030955	molecular_function	potassium ion binding
A	0031402	molecular_function	sodium ion binding
A	0036376	biological_process	sodium ion export across plasma membrane
A	0042383	cellular_component	sarcolemma
A	0042470	cellular_component	melanosome
A	0042995	cellular_component	cell projection
A	0046872	molecular_function	metal ion binding
A	0051117	molecular_function	ATPase binding
A	0055085	biological_process	transmembrane transport
A	0086009	biological_process	membrane repolarization
A	1902600	biological_process	proton transmembrane transport
A	1990573	biological_process	potassium ion import across plasma membrane
B	0001671	molecular_function	ATPase activator activity
B	0005391	molecular_function	P-type sodium:potassium-exchanging transporter activity
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
B	0006813	biological_process	potassium ion transport
B	0006814	biological_process	sodium ion transport
B	0006883	biological_process	intracellular sodium ion homeostasis
B	0007155	biological_process	cell adhesion
B	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
B	0016324	cellular_component	apical plasma membrane
B	0030007	biological_process	intracellular potassium ion homeostasis
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0036376	biological_process	sodium ion export across plasma membrane
B	0042383	cellular_component	sarcolemma
B	0045087	biological_process	innate immune response
B	0051117	molecular_function	ATPase binding
B	0055085	biological_process	transmembrane transport
B	0086009	biological_process	membrane repolarization
B	1902600	biological_process	proton transmembrane transport
B	1903278	biological_process	positive regulation of sodium ion export across plasma membrane
B	1903288	biological_process	positive regulation of potassium ion import across plasma membrane
B	1903408	biological_process	positive regulation of P-type sodium:potassium-exchanging transporter activity
B	1990573	biological_process	potassium ion import across plasma membrane
C	0000166	molecular_function	nucleotide binding
C	0002028	biological_process	regulation of sodium ion transport
C	0005215	molecular_function	transporter activity
C	0005391	molecular_function	P-type sodium:potassium-exchanging transporter activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005886	cellular_component	plasma membrane
C	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
C	0006813	biological_process	potassium ion transport
C	0006814	biological_process	sodium ion transport
C	0006883	biological_process	intracellular sodium ion homeostasis
C	0008556	molecular_function	P-type potassium transmembrane transporter activity
C	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
C	0016020	cellular_component	membrane
C	0016323	cellular_component	basolateral plasma membrane
C	0016887	molecular_function	ATP hydrolysis activity
C	0030007	biological_process	intracellular potassium ion homeostasis
C	0030424	cellular_component	axon
C	0030955	molecular_function	potassium ion binding
C	0031402	molecular_function	sodium ion binding
C	0036376	biological_process	sodium ion export across plasma membrane
C	0042383	cellular_component	sarcolemma
C	0042470	cellular_component	melanosome
C	0042995	cellular_component	cell projection
C	0046872	molecular_function	metal ion binding
C	0051117	molecular_function	ATPase binding
C	0055085	biological_process	transmembrane transport
C	0086009	biological_process	membrane repolarization
C	1902600	biological_process	proton transmembrane transport
C	1990573	biological_process	potassium ion import across plasma membrane
D	0001671	molecular_function	ATPase activator activity
D	0005391	molecular_function	P-type sodium:potassium-exchanging transporter activity
D	0005515	molecular_function	protein binding
D	0005886	cellular_component	plasma membrane
D	0005890	cellular_component	sodium:potassium-exchanging ATPase complex
D	0006813	biological_process	potassium ion transport
D	0006814	biological_process	sodium ion transport
D	0006883	biological_process	intracellular sodium ion homeostasis
D	0007155	biological_process	cell adhesion
D	0010248	biological_process	establishment or maintenance of transmembrane electrochemical gradient
D	0016324	cellular_component	apical plasma membrane
D	0030007	biological_process	intracellular potassium ion homeostasis
D	0030674	molecular_function	protein-macromolecule adaptor activity
D	0036376	biological_process	sodium ion export across plasma membrane
D	0042383	cellular_component	sarcolemma
D	0045087	biological_process	innate immune response
D	0051117	molecular_function	ATPase binding
D	0055085	biological_process	transmembrane transport
D	0086009	biological_process	membrane repolarization
D	1902600	biological_process	proton transmembrane transport
D	1903278	biological_process	positive regulation of sodium ion export across plasma membrane
D	1903288	biological_process	positive regulation of potassium ion import across plasma membrane
D	1903408	biological_process	positive regulation of P-type sodium:potassium-exchanging transporter activity
D	1990573	biological_process	potassium ion import across plasma membrane
E	0016020	cellular_component	membrane
E	0043269	biological_process	regulation of monoatomic ion transport
E	0099106	molecular_function	ion channel regulator activity
G	0016020	cellular_component	membrane
G	0043269	biological_process	regulation of monoatomic ion transport
G	0099106	molecular_function	ion channel regulator activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE OBN A 1

Chain	Residue
A	GLN111
A	PRO118
A	ASP121
A	ASN122
A	VAL322
A	ALA323
A	PHE783
A	THR797
A	ARG880

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE OBN C 1

Chain	Residue
C	GLN111
C	PRO118
C	ASP121
C	ASN122
C	GLY319
C	VAL322
C	ALA323
C	PHE783
C	THR797
C	ARG880

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 1017

Chain	Residue
A	PHD369
A	THR371
A	ASP710
A	GLY711

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG C 1017

Chain	Residue
C	PHD369
C	THR371
C	ASP710

---

Functional Information from PROSITE/UniProt

site_id	PS00154
Number of Residues	7
Details	ATPASE_E1_E2 E1-E2 ATPases phosphorylation site. DKTGTLT

Chain	Residue	Details
A	PHD369-THR375

---

site_id	PS00391
Number of Residues	16
Details	ATPASE_NA_K_BETA_2 Sodium and potassium ATPases beta subunits signature 2. RkvCRfrlewLgnCSG

Chain	Residue	Details
B	ARG146-GLY161

---

site_id	PS01310
Number of Residues	14
Details	FXYD FXYD family signature. DpFyYDyeTVRngG

Chain	Residue	Details
G	ASP17-GLY30

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	54
Details	TRANSMEM: Helical; Signal-anchor for type II membrane protein => ECO:0000255

Chain	Residue	Details
B	ILE35-SER62
A	THR979-LYS999
C	GLU81-ALA101
C	LEU125-ALA145
C	GLU282-ILE301
C	VAL314-ALA331
C	LYS766-ILE785
C	GLY796-ALA816
C	LEU837-PHE859
C	HIS912-LYS931
C	LYS945-TYR963
D	ILE35-SER62
C	THR979-LYS999
A	GLU282-ILE301
A	VAL314-ALA331
A	LYS766-ILE785
A	GLY796-ALA816
A	LEU837-PHE859
A	HIS912-LYS931
A	LYS945-TYR963

---

site_id	SWS_FT_FI2
Number of Residues	480
Details	TOPO_DOM: Extracellular => ECO:0000255

Chain	Residue	Details
B	GLU63-SER303
C	CYS964-PRO978
D	GLU63-SER303
A	PHE786-LEU795
A	PHE860-CYS911
A	CYS964-PRO978
C	ILE102-TYR124
C	LEU302-ALA313
C	PHE786-LEU795
C	PHE860-CYS911

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P14094

Chain	Residue	Details
B	TYR101
C	LEU1000-TYR1016
D	TYR101
A	TYR817-LYS836
A	THR932-ASN944
A	LEU1000-TYR1016
C	LYS146-ILE281
C	THR332-LEU765
C	TYR817-LYS836
C	THR932-ASN944

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000250

Chain	Residue	Details
B	ASN158
B	ASN193
B	ASN265
D	ASN158
D	ASN193
D	ASN265

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	LYS480
A	ASP710
A	ASP714
C	LYS480
C	ASP710
C	ASP714

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P06685

Chain	Residue	Details
A	SER33
A	SER40
A	SER445
A	SER477
C	SER33
C	SER40
C	SER445
C	SER477

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q8VDN2

Chain	Residue	Details
A	SER221
A	SER661
A	SER668
C	SER221
C	SER661
C	SER668

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:Q8VDN2

Chain	Residue	Details
A	TYR253
C	TYR253

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P05023

Chain	Residue	Details
A	TYR535
C	TYR535

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q8VDN2

Chain	Residue	Details
A	LYS654
C	LYS654

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PKA => ECO:0000250|UniProtKB:P06685

Chain	Residue	Details
A	SER936
C	SER936

---