3LQ0
Zymogen structure of crayfish astacin metallopeptidase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004222 | molecular_function | metalloendopeptidase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006508 | biological_process | proteolysis |
| A | 0007155 | biological_process | cell adhesion |
| A | 0008233 | molecular_function | peptidase activity |
| A | 0008237 | molecular_function | metallopeptidase activity |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0009566 | biological_process | fertilization |
| A | 0010954 | biological_process | positive regulation of protein processing |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0060468 | biological_process | prevention of polyspermy |
| A | 0060473 | cellular_component | cortical granule |
| A | 0070001 | molecular_function | aspartic-type peptidase activity |
| A | 0070002 | molecular_function | glutamic-type peptidase activity |
| A | 2000360 | biological_process | negative regulation of binding of sperm to zona pellucida |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ZN A 999 |
| Chain | Residue |
| A | ASP21 |
| A | HIS92 |
| A | HIS96 |
| A | HIS102 |
| A | TYR149 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL A 501 |
| Chain | Residue |
| A | HOH540 |
| A | HOH543 |
| A | HOH706 |
| A | HOH771 |
| A | ALA7 |
| A | TYR86 |
| A | TYR177 |
| A | HOH516 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 502 |
| Chain | Residue |
| A | LEU31 |
| A | GLU38 |
| A | PHE45 |
| A | HOH511 |
| A | HOH520 |
| A | HOH719 |
| A | HOH733 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 503 |
| Chain | Residue |
| A | TYR54 |
| A | GLU56 |
| A | ALA74 |
| A | ARG135 |
| A | HOH606 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 504 |
| Chain | Residue |
| A | SER23 |
| A | GLY24 |
| A | LYS151 |
| A | TYR152 |
| A | ASP172 |
| A | HOH695 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 33 |
| Details | Propeptide: {"description":"Activation peptide","featureId":"PRO_0000028872","evidences":[{"source":"PubMed","id":"3548817","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 198 |
| Details | Domain: {"description":"Peptidase M12A","evidences":[{"source":"PROSITE-ProRule","id":"PRU01211","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01211","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1319561","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01211","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1319561","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8756323","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 403 |
| Chain | Residue | Details |
| A | HIS92 | metal ligand |
| A | ALA93 | proton shuttle (general acid/base) |
| A | HIS96 | metal ligand |
| A | HIS102 | metal ligand |
| A | TYR149 | transition state stabiliser |
