3LKZ
Structural and functional analyses of a conserved hydrophobic pocket of flavivirus methyltransferase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003968 | molecular_function | RNA-dependent RNA polymerase activity |
| A | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| A | 0004483 | molecular_function | mRNA (nucleoside-2'-O-)-methyltransferase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0032259 | biological_process | methylation |
| B | 0003968 | molecular_function | RNA-dependent RNA polymerase activity |
| B | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| B | 0004483 | molecular_function | mRNA (nucleoside-2'-O-)-methyltransferase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0032259 | biological_process | methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE SFG A 301 |
| Chain | Residue |
| A | SER56 |
| A | GLU111 |
| A | VAL130 |
| A | ASP131 |
| A | VAL132 |
| A | PHE133 |
| A | ASP146 |
| A | ILE147 |
| A | GLU149 |
| A | HOH376 |
| A | HOH396 |
| A | GLY58 |
| A | HOH425 |
| B | ARG200 |
| A | GLY81 |
| A | CYS82 |
| A | GLY83 |
| A | GLY86 |
| A | TRP87 |
| A | LYS105 |
| A | HIS110 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 302 |
| Chain | Residue |
| A | HIS159 |
| A | LYS246 |
| A | ARG247 |
| A | THR248 |
| A | HOH463 |
| A | HOH496 |
| B | ARG177 |
| site_id | AC3 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE SFG B 301 |
| Chain | Residue |
| A | ARG200 |
| B | SER56 |
| B | GLY58 |
| B | GLY81 |
| B | CYS82 |
| B | GLY83 |
| B | GLY86 |
| B | TRP87 |
| B | THR104 |
| B | LYS105 |
| B | HIS110 |
| B | GLU111 |
| B | VAL130 |
| B | ASP131 |
| B | VAL132 |
| B | PHE133 |
| B | ASP146 |
| B | GLU149 |
| B | HOH386 |
| B | HOH397 |
| B | HOH512 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL B 302 |
| Chain | Residue |
| A | ARG177 |
| B | HIS159 |
| B | LYS246 |
| B | ARG247 |
| B | THR248 |
| B | HOH483 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | ACT_SITE: For 2'-O-MTase activity => ECO:0000269|PubMed:17267492 |
| Chain | Residue | Details |
| A | LYS61 | |
| A | ASP146 | |
| A | LYS182 | |
| A | GLU218 | |
| B | LYS61 | |
| B | ASP146 | |
| B | LYS182 | |
| B | GLU218 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:17267492 |
| Chain | Residue | Details |
| A | SER56 | |
| B | SER56 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0007744|PDB:2OY0 |
| Chain | Residue | Details |
| A | GLY86 | |
| A | TRP87 | |
| A | ASP131 | |
| A | VAL132 | |
| B | GLY86 | |
| B | TRP87 | |
| B | ASP131 | |
| B | VAL132 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924 |
| Chain | Residue | Details |
| A | THR104 | |
| A | LYS105 | |
| A | ILE147 | |
| A | TYR220 | |
| B | THR104 | |
| B | LYS105 | |
| B | ILE147 | |
| B | TYR220 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17267492 |
| Chain | Residue | Details |
| A | GLU111 | |
| A | ASP146 | |
| B | GLU111 | |
| B | ASP146 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 12 |
| Details | SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924 |
| Chain | Residue | Details |
| A | LYS13 | |
| B | SER150 | |
| B | ARG213 | |
| B | SER215 | |
| A | ASN17 | |
| A | ARG28 | |
| A | SER150 | |
| A | ARG213 | |
| A | SER215 | |
| B | LYS13 | |
| B | ASN17 | |
| B | ARG28 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | SITE: mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924 |
| Chain | Residue | Details |
| A | LEU16 | |
| A | MET19 | |
| B | LEU16 | |
| B | MET19 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:17267492 |
| Chain | Residue | Details |
| A | PHE24 | |
| B | PHE24 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 6 |
| Details | SITE: Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 |
| Chain | Residue | Details |
| A | LYS61 | |
| A | LYS182 | |
| A | GLU218 | |
| B | LYS61 | |
| B | LYS182 | |
| B | GLU218 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | SITE: Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924 |
| Chain | Residue | Details |
| A | ASP146 | |
| B | ASP146 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P03314 |
| Chain | Residue | Details |
| A | SER56 | |
| B | SER56 |
