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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

3K0F

Crystal structure of the phosphorylation-site double mutant T426A/T432A of the KaiC circadian clock protein

Functional Information from GO Data
ChainGOidnamespacecontents
A0000166molecular_functionnucleotide binding
A0000287molecular_functionmagnesium ion binding
A0003677molecular_functionDNA binding
A0004674molecular_functionprotein serine/threonine kinase activity
A0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0006355biological_processregulation of DNA-templated transcription
A0007623biological_processcircadian rhythm
A0009649biological_processentrainment of circadian clock
A0016301molecular_functionkinase activity
A0016740molecular_functiontransferase activity
A0016787molecular_functionhydrolase activity
A0016887molecular_functionATP hydrolysis activity
A0042752biological_processregulation of circadian rhythm
A0042754biological_processnegative regulation of circadian rhythm
A0042802molecular_functionidentical protein binding
A0046872molecular_functionmetal ion binding
A0048511biological_processrhythmic process
A0070297biological_processregulation of phosphorelay signal transduction system
A0106310molecular_functionprotein serine kinase activity
B0000166molecular_functionnucleotide binding
B0000287molecular_functionmagnesium ion binding
B0003677molecular_functionDNA binding
B0004674molecular_functionprotein serine/threonine kinase activity
B0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0006355biological_processregulation of DNA-templated transcription
B0007623biological_processcircadian rhythm
B0009649biological_processentrainment of circadian clock
B0016301molecular_functionkinase activity
B0016740molecular_functiontransferase activity
B0016787molecular_functionhydrolase activity
B0016887molecular_functionATP hydrolysis activity
B0042752biological_processregulation of circadian rhythm
B0042754biological_processnegative regulation of circadian rhythm
B0042802molecular_functionidentical protein binding
B0046872molecular_functionmetal ion binding
B0048511biological_processrhythmic process
B0070297biological_processregulation of phosphorelay signal transduction system
B0106310molecular_functionprotein serine kinase activity
C0000166molecular_functionnucleotide binding
C0000287molecular_functionmagnesium ion binding
C0003677molecular_functionDNA binding
C0004674molecular_functionprotein serine/threonine kinase activity
C0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
C0005515molecular_functionprotein binding
C0005524molecular_functionATP binding
C0006355biological_processregulation of DNA-templated transcription
C0007623biological_processcircadian rhythm
C0009649biological_processentrainment of circadian clock
C0016301molecular_functionkinase activity
C0016740molecular_functiontransferase activity
C0016787molecular_functionhydrolase activity
C0016887molecular_functionATP hydrolysis activity
C0042752biological_processregulation of circadian rhythm
C0042754biological_processnegative regulation of circadian rhythm
C0042802molecular_functionidentical protein binding
C0046872molecular_functionmetal ion binding
C0048511biological_processrhythmic process
C0070297biological_processregulation of phosphorelay signal transduction system
C0106310molecular_functionprotein serine kinase activity
D0000166molecular_functionnucleotide binding
D0000287molecular_functionmagnesium ion binding
D0003677molecular_functionDNA binding
D0004674molecular_functionprotein serine/threonine kinase activity
D0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
D0005515molecular_functionprotein binding
D0005524molecular_functionATP binding
D0006355biological_processregulation of DNA-templated transcription
D0007623biological_processcircadian rhythm
D0009649biological_processentrainment of circadian clock
D0016301molecular_functionkinase activity
D0016740molecular_functiontransferase activity
D0016787molecular_functionhydrolase activity
D0016887molecular_functionATP hydrolysis activity
D0042752biological_processregulation of circadian rhythm
D0042754biological_processnegative regulation of circadian rhythm
D0042802molecular_functionidentical protein binding
D0046872molecular_functionmetal ion binding
D0048511biological_processrhythmic process
D0070297biological_processregulation of phosphorelay signal transduction system
D0106310molecular_functionprotein serine kinase activity
E0000166molecular_functionnucleotide binding
E0000287molecular_functionmagnesium ion binding
E0003677molecular_functionDNA binding
E0004674molecular_functionprotein serine/threonine kinase activity
E0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
E0005515molecular_functionprotein binding
E0005524molecular_functionATP binding
E0006355biological_processregulation of DNA-templated transcription
E0007623biological_processcircadian rhythm
E0009649biological_processentrainment of circadian clock
E0016301molecular_functionkinase activity
E0016740molecular_functiontransferase activity
E0016787molecular_functionhydrolase activity
E0016887molecular_functionATP hydrolysis activity
E0042752biological_processregulation of circadian rhythm
E0042754biological_processnegative regulation of circadian rhythm
E0042802molecular_functionidentical protein binding
E0046872molecular_functionmetal ion binding
E0048511biological_processrhythmic process
E0070297biological_processregulation of phosphorelay signal transduction system
E0106310molecular_functionprotein serine kinase activity
F0000166molecular_functionnucleotide binding
F0000287molecular_functionmagnesium ion binding
F0003677molecular_functionDNA binding
F0004674molecular_functionprotein serine/threonine kinase activity
F0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
F0005515molecular_functionprotein binding
F0005524molecular_functionATP binding
F0006355biological_processregulation of DNA-templated transcription
F0007623biological_processcircadian rhythm
F0009649biological_processentrainment of circadian clock
F0016301molecular_functionkinase activity
F0016740molecular_functiontransferase activity
F0016787molecular_functionhydrolase activity
F0016887molecular_functionATP hydrolysis activity
F0042752biological_processregulation of circadian rhythm
F0042754biological_processnegative regulation of circadian rhythm
F0042802molecular_functionidentical protein binding
F0046872molecular_functionmetal ion binding
F0048511biological_processrhythmic process
F0070297biological_processregulation of phosphorelay signal transduction system
F0106310molecular_functionprotein serine kinase activity
Functional Information from PDB Data
site_idAC1
Number of Residues5
DetailsBINDING SITE FOR RESIDUE MG A 801
ChainResidue
ATHR295
AGLU318
AHOH523
AATP901
BARG459
site_idAC2
Number of Residues23
DetailsBINDING SITE FOR RESIDUE ATP A 901
ChainResidue
ALYS294
ATHR295
ALEU296
AGLU318
ATRP331
AARG451
AILE472
AHOH523
AHOH541
AMG801
BALA432
BLYS457
BMET458
BARG459
BGLY460
BSER461
BTRP462
BHIS463
BLYS465
ATHR290
AGLY291
ATHR292
AGLY293
site_idAC3
Number of Residues21
DetailsBINDING SITE FOR RESIDUE ATP A 903
ChainResidue
AGLY49
ATHR50
AGLY51
ALYS52
ATHR53
ALEU54
ASER89
APHE90
AARG218
AILE239
ATHR240
AASP241
AMG521
AHOH538
BLYS224
BLEU225
BARG226
BTHR228
BSER229
BHIS230
BLYS232
site_idAC4
Number of Residues3
DetailsBINDING SITE FOR RESIDUE MG A 521
ChainResidue
ATHR53
AASP145
AATP903
site_idAC5
Number of Residues3
DetailsBINDING SITE FOR RESIDUE MG B 802
ChainResidue
BTHR295
BGLU318
BATP901
site_idAC6
Number of Residues21
DetailsBINDING SITE FOR RESIDUE ATP B 901
ChainResidue
BTHR290
BGLY291
BTHR292
BGLY293
BLYS294
BTHR295
BLEU296
BGLU318
BSER330
BTRP331
BARG451
BILE472
BASP474
BMG802
CLYS457
CMET458
CARG459
CSER461
CTRP462
CHIS463
CLYS465
site_idAC7
Number of Residues18
DetailsBINDING SITE FOR RESIDUE ATP B 903
ChainResidue
BGLY49
BTHR50
BGLY51
BLYS52
BTHR53
BLEU54
BSER89
BPHE90
BILE239
BASP241
BMG521
CLYS224
CLEU225
CARG226
CTHR228
CSER229
CHIS230
CLYS232
site_idAC8
Number of Residues3
DetailsBINDING SITE FOR RESIDUE MG C 803
ChainResidue
CTHR295
CGLU318
CATP901
site_idAC9
Number of Residues3
DetailsBINDING SITE FOR RESIDUE MG B 521
ChainResidue
BATP903
BTHR53
BASP145
site_idBC1
Number of Residues23
DetailsBINDING SITE FOR RESIDUE ATP C 901
ChainResidue
CTHR290
CGLY291
CTHR292
CGLY293
CLYS294
CTHR295
CLEU296
CGLU318
CSER330
CTRP331
CARG451
CILE472
CSER473
CHOH541
CMG803
DLYS457
DMET458
DARG459
DSER461
DTRP462
DHIS463
DLYS465
DHOH536
site_idBC2
Number of Residues17
DetailsBINDING SITE FOR RESIDUE ATP C 903
ChainResidue
CGLY49
CTHR50
CGLY51
CLYS52
CTHR53
CLEU54
CGLU78
CSER89
CILE239
CMG522
CHOH530
DPHE199
DLYS224
DARG226
DGLY227
DTHR228
DHIS230
site_idBC3
Number of Residues4
DetailsBINDING SITE FOR RESIDUE MG D 804
ChainResidue
DTHR295
DGLU319
DHOH524
DATP901
site_idBC4
Number of Residues20
DetailsBINDING SITE FOR RESIDUE ATP D 901
ChainResidue
DTHR290
DGLY291
DTHR292
DGLY293
DLYS294
DTHR295
DLEU296
DGLU318
DSER330
DTRP331
DARG451
DILE472
DSER473
DHOH524
DMG804
ELYS457
EMET458
EARG459
ESER461
EHIS463
site_idBC5
Number of Residues3
DetailsBINDING SITE FOR RESIDUE MG C 522
ChainResidue
CTHR53
CASP145
CATP903
site_idBC6
Number of Residues19
DetailsBINDING SITE FOR RESIDUE ATP D 903
ChainResidue
DGLY49
DTHR50
DGLY51
DLYS52
DTHR53
DLEU54
DGLU78
DSER89
DPHE90
DARG218
DILE239
DASP241
DHOH521
ELYS224
ELEU225
EARG226
ETHR228
EHIS230
ELYS232
site_idBC7
Number of Residues4
DetailsBINDING SITE FOR RESIDUE MG E 805
ChainResidue
ETHR295
EGLU319
EATP901
FHOH526
site_idBC8
Number of Residues23
DetailsBINDING SITE FOR RESIDUE ATP E 901
ChainResidue
ETHR290
EGLY291
ETHR292
EGLY293
ELYS294
ETHR295
ELEU296
ESER330
ETRP331
EARG451
EILE472
ESER473
EHOH553
EMG805
FLYS457
FMET458
FARG459
FGLY460
FSER461
FTRP462
FHIS463
FLYS465
FHOH526
site_idBC9
Number of Residues19
DetailsBINDING SITE FOR RESIDUE ATP E 903
ChainResidue
EGLY49
ETHR50
EGLY51
ELYS52
ETHR53
ELEU54
EGLU78
ESER89
EILE239
ETHR240
EASP241
EHOH538
FLYS224
FLEU225
FARG226
FTHR228
FSER229
FHIS230
FLYS232
site_idCC1
Number of Residues2
DetailsBINDING SITE FOR RESIDUE MG F 806
ChainResidue
FTHR295
FATP901
site_idCC2
Number of Residues22
DetailsBINDING SITE FOR RESIDUE ATP F 901
ChainResidue
ALYS457
AMET458
AARG459
AGLY460
ASER461
ATRP462
AHIS463
ALYS465
FTHR290
FGLY291
FTHR292
FGLY293
FLYS294
FTHR295
FLEU296
FGLU318
FSER330
FTRP331
FARG451
FILE472
FHOH523
FMG806
site_idCC3
Number of Residues17
DetailsBINDING SITE FOR RESIDUE ATP F 903
ChainResidue
ALYS224
ALEU225
AARG226
ATHR228
AHIS230
FGLY49
FTHR50
FGLY51
FLYS52
FTHR53
FLEU54
FGLU78
FSER89
FPHE90
FILE239
FTHR240
FASP241
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues258
DetailsDomain: {"description":"KaiC 2","evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15347809","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15347812","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI2
Number of Residues42
DetailsRegion: {"description":"B-loop, required to bind KaiB and SasA","evidences":[{"source":"UniProtKB","id":"Q79V60","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI3
Number of Residues72
DetailsRegion: {"description":"Linker","evidences":[{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI4
Number of Residues45
DetailsRegion: {"description":"A-loop, interacts with KaiA","evidences":[{"source":"PubMed","id":"18728181","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI5
Number of Residues6
DetailsActive site: {"description":"Proton acceptor in CI (KaiC 1)","evidences":[{"source":"PubMed","id":"22304631","evidenceCode":"ECO:0000305"}]}
ChainResidueDetails
site_idSWS_FT_FI6
Number of Residues6
DetailsActive site: {"description":"Proton acceptor in CII (KaiC 2)","evidences":[{"source":"PubMed","id":"22304631","evidenceCode":"ECO:0000305"}]}
ChainResidueDetails
site_idSWS_FT_FI7
Number of Residues150
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16628225","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22304631","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1TF7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DUG","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI8
Number of Residues6
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PDB","id":"4DUG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4TL6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7DXQ","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI9
Number of Residues12
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16628225","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1TF7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI10
Number of Residues6
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16628225","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1U9I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DUG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7DXQ","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI11
Number of Residues6
DetailsBinding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16628225","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI12
Number of Residues6
DetailsModified residue: {"description":"Phosphoserine; by autocatalysis","evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15347809","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15347812","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17717528","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17916691","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1U9I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI13
Number of Residues6
DetailsModified residue: {"description":"Phosphothreonine; by autocatalysis","evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15347809","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15347812","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17717528","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17916691","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1U9I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails

242842

PDB entries from 2025-10-08

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