3K0F
Crystal structure of the phosphorylation-site double mutant T426A/T432A of the KaiC circadian clock protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 113.15 |
| Detector technology | CCD |
| Collection date | 2008-11-18 |
| Detector | MAR 300MM CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 132.927, 135.415, 205.618 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbl |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.061 | 0.427 |
| Number of reflections | 75064 | |
| <I/σ(I)> | 23.4 | 2.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 291 | SODIUM FORMATE, GLYCEROL, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
