3JA6

Cryo-electron Tomography and All-atom Molecular Dynamics Simulations Reveal a Novel Kinase Conformational Switch in Bacterial Chemotaxis Signaling

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006935	biological_process	chemotaxis
A	0007165	biological_process	signal transduction
A	0019904	molecular_function	protein domain specific binding
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006935	biological_process	chemotaxis
B	0007165	biological_process	signal transduction
B	0019904	molecular_function	protein domain specific binding
C	0000155	molecular_function	phosphorelay sensor kinase activity
C	0000160	biological_process	phosphorelay signal transduction system
C	0004673	molecular_function	protein histidine kinase activity
C	0005737	cellular_component	cytoplasm
C	0006935	biological_process	chemotaxis
C	0007165	biological_process	signal transduction
C	0016310	biological_process	phosphorylation
C	0016772	molecular_function	transferase activity, transferring phosphorus-containing groups
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006935	biological_process	chemotaxis
D	0007165	biological_process	signal transduction
D	0019904	molecular_function	protein domain specific binding
E	0000155	molecular_function	phosphorelay sensor kinase activity
E	0000160	biological_process	phosphorelay signal transduction system
E	0004673	molecular_function	protein histidine kinase activity
E	0005737	cellular_component	cytoplasm
E	0006935	biological_process	chemotaxis
E	0007165	biological_process	signal transduction
E	0016310	biological_process	phosphorylation
E	0016772	molecular_function	transferase activity, transferring phosphorus-containing groups
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006935	biological_process	chemotaxis
F	0007165	biological_process	signal transduction
F	0019904	molecular_function	protein domain specific binding
G	0004888	molecular_function	transmembrane signaling receptor activity
G	0006935	biological_process	chemotaxis
G	0007165	biological_process	signal transduction
G	0016020	cellular_component	membrane
H	0004888	molecular_function	transmembrane signaling receptor activity
H	0006935	biological_process	chemotaxis
H	0007165	biological_process	signal transduction
H	0016020	cellular_component	membrane
I	0004888	molecular_function	transmembrane signaling receptor activity
I	0006935	biological_process	chemotaxis
I	0007165	biological_process	signal transduction
I	0016020	cellular_component	membrane
J	0004888	molecular_function	transmembrane signaling receptor activity
J	0006935	biological_process	chemotaxis
J	0007165	biological_process	signal transduction
J	0016020	cellular_component	membrane
K	0004888	molecular_function	transmembrane signaling receptor activity
K	0006935	biological_process	chemotaxis
K	0007165	biological_process	signal transduction
K	0016020	cellular_component	membrane
L	0004888	molecular_function	transmembrane signaling receptor activity
L	0006935	biological_process	chemotaxis
L	0007165	biological_process	signal transduction
L	0016020	cellular_component	membrane
M	0004888	molecular_function	transmembrane signaling receptor activity
M	0006935	biological_process	chemotaxis
M	0007165	biological_process	signal transduction
M	0016020	cellular_component	membrane
N	0004888	molecular_function	transmembrane signaling receptor activity
N	0006935	biological_process	chemotaxis
N	0007165	biological_process	signal transduction
N	0016020	cellular_component	membrane
O	0004888	molecular_function	transmembrane signaling receptor activity
O	0006935	biological_process	chemotaxis
O	0007165	biological_process	signal transduction
O	0016020	cellular_component	membrane
P	0004888	molecular_function	transmembrane signaling receptor activity
P	0006935	biological_process	chemotaxis
P	0007165	biological_process	signal transduction
P	0016020	cellular_component	membrane
Q	0004888	molecular_function	transmembrane signaling receptor activity
Q	0006935	biological_process	chemotaxis
Q	0007165	biological_process	signal transduction
Q	0016020	cellular_component	membrane
R	0004888	molecular_function	transmembrane signaling receptor activity
R	0006935	biological_process	chemotaxis
R	0007165	biological_process	signal transduction
R	0016020	cellular_component	membrane

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	804
Details	Domain: {"description":"CheW-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00052","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2832
Details	Domain: {"description":"Methyl-accepting transducer","evidences":[{"source":"PROSITE-ProRule","id":"PRU00284","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	Modified residue: {"description":"Glutamate methyl ester (Gln)","evidences":[{"source":"PubMed","id":"16707700","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	24
Details	Modified residue: {"description":"Glutamate methyl ester (Glu)","evidences":[{"source":"PubMed","id":"16707700","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	Modified residue: {"description":"Deamidated glutamine","evidences":[{"source":"PubMed","id":"16707700","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 327

Chain	Residue	Details
C	ASN409	electrostatic stabiliser, hydrogen bond donor, metal ligand, steric role

---

site_id	MCSA2
Number of Residues	1
Details	M-CSA 327

Chain	Residue	Details
E	ASN409	electrostatic stabiliser, hydrogen bond donor, metal ligand, steric role

---