3JA6
Cryo-electron Tomography and All-atom Molecular Dynamics Simulations Reveal a Novel Kinase Conformational Switch in Bacterial Chemotaxis Signaling
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | Single-sided blotting to avoid disruption of the monolayer |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 60 |
3D Reconstruction
| Method | TOMOGRAPHY |
| Resolution (Å) | 12.7 |
| Symmetry | C2 |
