3ISS
Crystal structure of enolpyruvyl-UDP-GlcNAc synthase (MurA):UDP-N-acetylmuramic acid:phosphite from Escherichia coli
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0008360 | biological_process | regulation of cell shape |
| A | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| A | 0009252 | biological_process | peptidoglycan biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| A | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| A | 0051301 | biological_process | cell division |
| A | 0071555 | biological_process | cell wall organization |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0008360 | biological_process | regulation of cell shape |
| B | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| B | 0009252 | biological_process | peptidoglycan biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| B | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| B | 0051301 | biological_process | cell division |
| B | 0071555 | biological_process | cell wall organization |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0008360 | biological_process | regulation of cell shape |
| C | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| C | 0009252 | biological_process | peptidoglycan biosynthetic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| C | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| C | 0051301 | biological_process | cell division |
| C | 0071555 | biological_process | cell wall organization |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0008360 | biological_process | regulation of cell shape |
| D | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| D | 0009252 | biological_process | peptidoglycan biosynthetic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| D | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| D | 0051301 | biological_process | cell division |
| D | 0071555 | biological_process | cell wall organization |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0008360 | biological_process | regulation of cell shape |
| E | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| E | 0009252 | biological_process | peptidoglycan biosynthetic process |
| E | 0016740 | molecular_function | transferase activity |
| E | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| E | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| E | 0051301 | biological_process | cell division |
| E | 0071555 | biological_process | cell wall organization |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0008360 | biological_process | regulation of cell shape |
| F | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| F | 0009252 | biological_process | peptidoglycan biosynthetic process |
| F | 0016740 | molecular_function | transferase activity |
| F | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| F | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| F | 0051301 | biological_process | cell division |
| F | 0071555 | biological_process | cell wall organization |
| G | 0003824 | molecular_function | catalytic activity |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005829 | cellular_component | cytosol |
| G | 0008360 | biological_process | regulation of cell shape |
| G | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| G | 0009252 | biological_process | peptidoglycan biosynthetic process |
| G | 0016740 | molecular_function | transferase activity |
| G | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| G | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| G | 0051301 | biological_process | cell division |
| G | 0071555 | biological_process | cell wall organization |
| H | 0003824 | molecular_function | catalytic activity |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0005829 | cellular_component | cytosol |
| H | 0008360 | biological_process | regulation of cell shape |
| H | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| H | 0009252 | biological_process | peptidoglycan biosynthetic process |
| H | 0016740 | molecular_function | transferase activity |
| H | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| H | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| H | 0051301 | biological_process | cell division |
| H | 0071555 | biological_process | cell wall organization |
| I | 0003824 | molecular_function | catalytic activity |
| I | 0005737 | cellular_component | cytoplasm |
| I | 0005829 | cellular_component | cytosol |
| I | 0008360 | biological_process | regulation of cell shape |
| I | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| I | 0009252 | biological_process | peptidoglycan biosynthetic process |
| I | 0016740 | molecular_function | transferase activity |
| I | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| I | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| I | 0051301 | biological_process | cell division |
| I | 0071555 | biological_process | cell wall organization |
| J | 0003824 | molecular_function | catalytic activity |
| J | 0005737 | cellular_component | cytoplasm |
| J | 0005829 | cellular_component | cytosol |
| J | 0008360 | biological_process | regulation of cell shape |
| J | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| J | 0009252 | biological_process | peptidoglycan biosynthetic process |
| J | 0016740 | molecular_function | transferase activity |
| J | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| J | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| J | 0051301 | biological_process | cell division |
| J | 0071555 | biological_process | cell wall organization |
| K | 0003824 | molecular_function | catalytic activity |
| K | 0005737 | cellular_component | cytoplasm |
| K | 0005829 | cellular_component | cytosol |
| K | 0008360 | biological_process | regulation of cell shape |
| K | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| K | 0009252 | biological_process | peptidoglycan biosynthetic process |
| K | 0016740 | molecular_function | transferase activity |
| K | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| K | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| K | 0051301 | biological_process | cell division |
| K | 0071555 | biological_process | cell wall organization |
| L | 0003824 | molecular_function | catalytic activity |
| L | 0005737 | cellular_component | cytoplasm |
| L | 0005829 | cellular_component | cytosol |
| L | 0008360 | biological_process | regulation of cell shape |
| L | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| L | 0009252 | biological_process | peptidoglycan biosynthetic process |
| L | 0016740 | molecular_function | transferase activity |
| L | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| L | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| L | 0051301 | biological_process | cell division |
| L | 0071555 | biological_process | cell wall organization |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PO3 A 501 |
| Chain | Residue |
| A | ARG91 |
| A | ARG397 |
| site_id | AC2 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE EPU A 601 |
| Chain | Residue |
| A | LEU124 |
| A | LYS160 |
| A | VAL161 |
| A | SER162 |
| A | VAL163 |
| A | GLY164 |
| A | ASP305 |
| A | VAL327 |
| A | PHE328 |
| A | LEU370 |
| A | HOH425 |
| A | HOH445 |
| A | HOH780 |
| A | LYS22 |
| A | ASN23 |
| A | ALA119 |
| A | ARG120 |
| A | PRO121 |
| A | VAL122 |
| A | ASP123 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO3 B 502 |
| Chain | Residue |
| B | ARG91 |
| B | CYS115 |
| B | ARG397 |
| site_id | AC4 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE EPU B 602 |
| Chain | Residue |
| B | LYS22 |
| B | ASN23 |
| B | ALA92 |
| B | TRP95 |
| B | ALA119 |
| B | ARG120 |
| B | PRO121 |
| B | VAL122 |
| B | ASP123 |
| B | LEU124 |
| B | HIS125 |
| B | LYS160 |
| B | SER162 |
| B | VAL163 |
| B | GLY164 |
| B | THR304 |
| B | ASP305 |
| B | VAL327 |
| B | PHE328 |
| B | LEU370 |
| B | ARG371 |
| B | HOH441 |
| B | HOH650 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PO3 C 502 |
| Chain | Residue |
| C | ARG91 |
| C | CYS115 |
| C | ARG120 |
| C | ARG397 |
| site_id | AC6 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE EPU C 603 |
| Chain | Residue |
| C | LYS22 |
| C | ASN23 |
| C | ALA119 |
| C | ARG120 |
| C | PRO121 |
| C | VAL122 |
| C | ASP123 |
| C | LEU124 |
| C | LYS160 |
| C | SER162 |
| C | VAL163 |
| C | GLY164 |
| C | ASP305 |
| C | VAL327 |
| C | PHE328 |
| C | LEU370 |
| C | HOH423 |
| C | HOH469 |
| C | HOH470 |
| C | HOH555 |
| C | HOH797 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PO3 D 503 |
| Chain | Residue |
| D | ARG91 |
| D | CYS115 |
| D | ARG120 |
| D | HOH663 |
| site_id | AC8 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE EPU D 604 |
| Chain | Residue |
| D | LYS22 |
| D | ASN23 |
| D | TRP95 |
| D | ALA119 |
| D | ARG120 |
| D | PRO121 |
| D | VAL122 |
| D | ASP123 |
| D | LEU124 |
| D | HIS125 |
| D | LYS160 |
| D | SER162 |
| D | VAL163 |
| D | GLY164 |
| D | ASP305 |
| D | VAL327 |
| D | PHE328 |
| D | LEU370 |
| D | HOH435 |
| D | HOH471 |
| site_id | AC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PO3 E 504 |
| Chain | Residue |
| E | CYS115 |
| E | ARG397 |
| site_id | BC1 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE EPU E 605 |
| Chain | Residue |
| E | LYS22 |
| E | ASN23 |
| E | TRP95 |
| E | ALA119 |
| E | ARG120 |
| E | PRO121 |
| E | VAL122 |
| E | ASP123 |
| E | LEU124 |
| E | LYS160 |
| E | SER162 |
| E | VAL163 |
| E | GLY164 |
| E | ASP305 |
| E | VAL327 |
| E | LEU370 |
| E | ARG371 |
| E | HOH442 |
| E | HOH456 |
| site_id | BC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PO3 F 506 |
| Chain | Residue |
| F | ARG91 |
| F | ARG397 |
| site_id | BC3 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE EPU F 606 |
| Chain | Residue |
| F | LYS22 |
| F | ASN23 |
| F | ALA119 |
| F | ARG120 |
| F | PRO121 |
| F | VAL122 |
| F | ASP123 |
| F | LEU124 |
| F | HIS125 |
| F | LYS160 |
| F | SER162 |
| F | VAL163 |
| F | GLY164 |
| F | ASP305 |
| F | VAL327 |
| F | PHE328 |
| F | LEU370 |
| F | HOH488 |
| F | HOH603 |
| site_id | BC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO3 G 507 |
| Chain | Residue |
| G | ARG91 |
| G | CYS115 |
| G | ARG397 |
| site_id | BC5 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE EPU G 607 |
| Chain | Residue |
| G | LYS22 |
| G | ASN23 |
| G | ALA92 |
| G | TRP95 |
| G | ALA119 |
| G | ARG120 |
| G | PRO121 |
| G | VAL122 |
| G | ASP123 |
| G | LEU124 |
| G | HIS125 |
| G | LYS160 |
| G | SER162 |
| G | VAL163 |
| G | GLY164 |
| G | ASP305 |
| G | VAL327 |
| G | ARG331 |
| G | LEU370 |
| site_id | BC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO3 H 508 |
| Chain | Residue |
| H | ARG91 |
| H | CYS115 |
| H | ARG397 |
| site_id | BC7 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE EPU H 608 |
| Chain | Residue |
| H | ASN23 |
| H | ALA119 |
| H | ARG120 |
| H | PRO121 |
| H | VAL122 |
| H | ASP123 |
| H | LEU124 |
| H | HIS125 |
| H | LYS160 |
| H | SER162 |
| H | VAL163 |
| H | GLY164 |
| H | ASP305 |
| H | VAL327 |
| H | PHE328 |
| H | ARG371 |
| H | HOH426 |
| H | HOH469 |
| site_id | BC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO3 I 509 |
| Chain | Residue |
| I | ARG91 |
| I | CYS115 |
| I | ARG397 |
| site_id | BC9 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE EPU I 609 |
| Chain | Residue |
| I | LYS22 |
| I | ASN23 |
| I | ALA119 |
| I | ARG120 |
| I | PRO121 |
| I | VAL122 |
| I | ASP123 |
| I | LEU124 |
| I | LYS160 |
| I | SER162 |
| I | VAL163 |
| I | GLY164 |
| I | ASP305 |
| I | VAL327 |
| I | PHE328 |
| I | LEU370 |
| I | HOH438 |
| site_id | CC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO3 J 510 |
| Chain | Residue |
| J | MET90 |
| J | ARG91 |
| J | CYS115 |
| J | ARG397 |
| J | HOH764 |
| site_id | CC2 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE EPU J 610 |
| Chain | Residue |
| J | LYS22 |
| J | ASN23 |
| J | TRP95 |
| J | ALA119 |
| J | ARG120 |
| J | PRO121 |
| J | VAL122 |
| J | ASP123 |
| J | LEU124 |
| J | HIS125 |
| J | LYS160 |
| J | SER162 |
| J | VAL163 |
| J | GLY164 |
| J | ASP305 |
| J | VAL327 |
| J | PHE328 |
| J | LEU370 |
| J | ARG371 |
| J | HOH443 |
| J | HOH673 |
| site_id | CC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO3 K 511 |
| Chain | Residue |
| K | ARG91 |
| K | CYS115 |
| K | ARG397 |
| site_id | CC4 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE EPU K 611 |
| Chain | Residue |
| K | LYS22 |
| K | ASN23 |
| K | ALA119 |
| K | ARG120 |
| K | PRO121 |
| K | VAL122 |
| K | ASP123 |
| K | LEU124 |
| K | HIS125 |
| K | LYS160 |
| K | SER162 |
| K | VAL163 |
| K | GLY164 |
| K | ASP305 |
| K | VAL327 |
| K | PHE328 |
| K | LEU370 |
| K | HOH424 |
| K | HOH425 |
| K | HOH427 |
| K | HOH444 |
| K | HOH457 |
| K | HOH477 |
| site_id | CC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PO3 L 512 |
| Chain | Residue |
| L | ARG91 |
| L | ARG397 |
| site_id | CC6 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE EPU L 612 |
| Chain | Residue |
| L | LYS22 |
| L | ASN23 |
| L | ALA119 |
| L | ARG120 |
| L | PRO121 |
| L | VAL122 |
| L | ASP123 |
| L | LEU124 |
| L | LYS160 |
| L | VAL161 |
| L | SER162 |
| L | VAL163 |
| L | GLY164 |
| L | ASP305 |
| L | VAL327 |
| L | PHE328 |
| L | LEU370 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 12 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"20392080","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8994972","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22378791","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1UAE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3KR6","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"20392080","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"22378791","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"3KR6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3SWD","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 24 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"20392080","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3KR6","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 96 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"20392080","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22378791","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3KR6","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | Modified residue: {"description":"2-(S-cysteinyl)pyruvic acid O-phosphothioketal","evidences":[{"source":"PubMed","id":"22378791","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| A | ASP305 | |
| A | ARG397 | |
| A | CYS115 | |
| A | ASN23 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| B | ASP305 | |
| B | ARG397 | |
| B | CYS115 | |
| B | ASN23 |
| site_id | CSA3 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| C | ASP305 | |
| C | ARG397 | |
| C | CYS115 | |
| C | ASN23 |
| site_id | CSA4 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| D | ASP305 | |
| D | ARG397 | |
| D | CYS115 | |
| D | ASN23 |
| site_id | CSA5 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| E | ASP305 | |
| E | ARG397 | |
| E | CYS115 | |
| E | ASN23 |
| site_id | CSA6 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| F | ASP305 | |
| F | ARG397 | |
| F | CYS115 | |
| F | ASN23 |
| site_id | CSA7 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| G | ASP305 | |
| G | ARG397 | |
| G | CYS115 | |
| G | ASN23 |
| site_id | CSA8 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| H | ASP305 | |
| H | ARG397 | |
| H | CYS115 | |
| H | ASN23 |
| site_id | CSA9 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| I | ASP305 | |
| I | ARG397 | |
| I | CYS115 | |
| I | ASN23 |
| site_id | CSA10 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| J | ASP305 | |
| J | ARG397 | |
| J | CYS115 | |
| J | ASN23 |
| site_id | CSA11 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| K | ASP305 | |
| K | ARG397 | |
| K | CYS115 | |
| K | ASN23 |
| site_id | CSA12 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1uae |
| Chain | Residue | Details |
| L | ASP305 | |
| L | ARG397 | |
| L | CYS115 | |
| L | ASN23 |
